Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,365,2380GC28.6% 91.0 / 13.6 57intergenic (‑109/‑10)csoR/CJ019_03165Copper‑sensing transcriptional repressor CsoR/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/12);  new base C (0/16);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.46e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCGAGTCAGCCAGTCGGGAGTGGCAACAA  >  ACB122/3365103‑3365372
                                                                                                                                        |                                                                                                                                      
cTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACTACCCCTTCAGAACAACCCTCCGCTTAGGCATCAAAAgc                                                                                                                                       >  8:302866/1‑136 (MQ=255)
   ttGGCGGTGTTCGTGATCGCTAATGCCTTGAGTCTCATGCATCGATTAATTACGCTCGCATCCTATCCCCCTGGGGGGT‑ATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGaacc                                                                                                                                    <  6:353236/136‑3 (MQ=255)
   ttGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTAATGCATCGATTAATTACGCTCGCATCCTATCCCCCTGGGGGGT‑ATATGTTAAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATAAAAAGAAac                                                                                                                                    <  6:174152/136‑1 (MQ=255)
      gCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACg                                                                                                                                 >  5:16871/1‑137 (MQ=255)
        gttgtTCGTGAGCGAGCATGCCTTGAGTCTCATGCATCTATGAATTACGCTCGCATCCAATCCCCCTGGGGGGG‑ATATGTACAAACCAACTCCCCCTCCAGAACAACCATCCGCTTAGGAATCAAAACAAACACGAc                                                                                                                               <  8:238423/135‑1 (MQ=255)
             tCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTGGCATCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACTACCACTTCCGCACAACCATCCGCTTAGGCATCAAAAGAAACACGTCCTGCt                                                                                                                          >  2:347659/1‑137 (MQ=255)
                                    ctcATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAgg                                                                                                   >  5:204732/1‑137 (MQ=255)
                                    ctcATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAgg                                                                                                   >  4:248674/1‑137 (MQ=255)
                                    ctcATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAgg                                                                                                   >  2:203278/1‑137 (MQ=255)
                                       aTGCATCGATGAATTACGCTCGCAGCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACTACCCCTTCATAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGa                                                                                                <  7:363717/137‑1 (MQ=255)
                                        tGCTTCTATTAATTACGCTGGCTTCCTAACCCGCTGGGGGGT‑ATTTGGTCAAACCAACTACCCCTTCATAACAACCATCCGCTTGGGCATCAAAATAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGAt                                                                                               <  2:124330/137‑1 (MQ=255)
                                          cATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGAttt                                                                                             >  1:133876/1‑137 (MQ=255)
                                             cGATAAATTACGCGCCCACCCTATCCCCGTGGGGGGT‑ATTTGGTCAACCCAACTCCCCCTACAGAACACCCATCCGATTAGGCAAAAAAACAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTg                                                                                          <  5:377006/137‑1 (MQ=255)
                                                tGAATTACGCTCGCATCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGacc                                                                                       >  5:328066/1‑137 (MQ=255)
                                                  aaTTACGCTCGCATCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCtt                                                                                     >  2:40448/1‑137 (MQ=255)
                                                  aaTTACGCTCGCATCCTATCCCCCT‑GGGGGG‑ATATTGTAAAACCAACTACCCCTTCAGAACAACCATCCGCTTAAGCATCAAAAGAAAACCGACATGCTCAGCAACCCCATCAGCACTAATGTTATTTTTgacattt                                                                                    >  6:270775/1‑131 (MQ=255)
                                                     tACGCTCGCATGCTAACGCCGGGGGGGGT‑ATATGGTCAACCAAACTCCCCCTTCAGAACCACCCCCCGGTTTGGCATAAAAACAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGct                                                                                  <  5:373538/137‑1 (MQ=255)
                                                       cgcccccATCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACACCCCATTCAGAACAACCATCCGCTTAGGCATCAAAACACACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGctct                                                                                <  4:119530/131‑1 (MQ=255)
                                                       cGCTCGCAACCTATCCCCCTGGGGGGT‑ATATGGTCAAACAAACTACCCCTTCACAACAACCATCCGCTTAGGCATCAAAACAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGctct                                                                                <  3:111002/137‑1 (MQ=255)
                                                            gCATCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCa                                                                           >  1:34342/1‑137 (MQ=255)
                                                              aTCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACTCCCCCTTCAGAACAACCATCCGCTTAGGCATCAAAACAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATc                                                                         <  2:61405/137‑1 (MQ=255)
                                                               aacatacccccGGGGGGGGTATATGTACAACCCAACTCCCCCTACACACCCACCCTCCCCTACGCCATCAAAACACCCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATc                                                                         <  3:264234/131‑1 (MQ=255)
                                                                   aTCCCCCGGGGGGGT‑ATATGGACAAACCACATACCCCTGCAGAACAACTATCCGCTTAGCCAACAAAACAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGAt                                                                    <  7:35837/137‑1 (MQ=255)
                                                                        cgtGGGGGGT‑TTATGGTAAAACCAACTACCCCTTCACAACAACCAACCGCTTAGCCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCt                                                               <  5:160882/135‑1 (MQ=255)
                                                                        cgtGGGGGGT‑ATATGGGCAAACCAACTACCCCTTCAGAACAACCTTCCTCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCt                                                               <  2:125415/135‑1 (MQ=255)
                                                                           ggggggg‑ATATGGTCAAACCAACTACCCCTTCAGAACACCCATCCGCTTAGGCATCAAAAGACACACGACATGCTCAGCACCCCCATCAGCAGTAAGGTGATTTTTGCCCTTGCTCTGGCAATCTTGATAGCCTggg                                                            >  6:229606/1‑137 (MQ=255)
                                                                             ggggg‑ATATGGTCAAACCAACAACCCCTACACACCACCCATCCGCTTCGGCATCAAAACAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGcc                                                          <  2:155363/137‑1 (MQ=255)
                                                                               ggg‑ATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCgg                                                        >  3:316588/1‑137 (MQ=255)
                                                                               ggg‑ATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCgg                                                        >  7:222903/1‑137 (MQ=255)
                                                                                     ttGGTCAAACCAACTCCCCCTTCATAACAACCATCCGCTAAGCCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGcaca                                                   <  3:333167/136‑1 (MQ=255)
                                                                                     atGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACACAATCAGAACTAAGGTGATTTTTGACCTTGCTCTTGCCATCTTGATAGCCTGTGCCTGGcaca                                                   >  4:37848/1‑137 (MQ=255)
                                                                                      tGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGCCATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCacac                                                  >  2:258816/1‑137 (MQ=255)
                                                                                      tGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCacac                                                  >  5:347631/1‑137 (MQ=255)
                                                                                       ggTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACAcc                                                 >  1:125415/1‑137 (MQ=255)
                                                                                        ctaaaaCCAACAACCCCTACAGAACAACCATCGGCTTCCCAAACAAAAGACACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACct                                                <  1:116772/134‑1 (MQ=255)
                                                                                          aaacccAACTACCCCTTCAAAACAACCATCCCCTTAGCCATCAAAACAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                                              <  7:39899/133‑1 (MQ=255)
                                                                                          aaaacaaacACCCCCTTCACAACAACCATCCGCTTCGGCATCAAAACACCCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                                              <  6:16871/136‑1 (MQ=255)
                                                                                          aaaaCCAACTACCCCTACAGAACAACCAACCGCTTAGTCATCAAAACAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                                              <  5:46038/136‑1 (MQ=255)
                                                                                          aaaaCCAACTACCCATTCACAACAACCATCAGATTAGCAAAAAAAACAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                                              <  1:345355/136‑1 (MQ=255)
                                                                                          aaaaCCAAATACCCCTAAACAACAACCAACCGATTCCGCAACAAAACAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                                              <  3:19474/136‑1 (MQ=255)
                                                                                          aaaaCAATCTACCCCTTCATAAAAACCATCCGTTTAGGAACCAAAAGAAACACGACATGCTCATCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCGATCTTGATAGCCTGGTCCGGGCACACctct                                              <  7:13595/136‑1 (MQ=255)
                                                                                           aaaCCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCACCCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTg                                             >  2:158308/1‑137 (MQ=255)
                                                                                           aaaCCAACAACCCCTTCACAACAACCATCCGATTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTg                                             <  5:170320/137‑1 (MQ=255)
                                                                                               cAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGct                                         >  8:163623/1‑137 (MQ=255)
                                                                                                    aCCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTa                                    >  1:284813/1‑137 (MQ=255)
                                                                                                      cccTTCACAACAACCATCCGCTTAGGCATCAAAACAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATg                                  <  4:168795/137‑1 (MQ=255)
                                                                                                      cccATCACAACAACCATCCGCTTAGCAATAAAAACAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATg                                  <  3:38899/137‑1 (MQ=255)
                                                                                                             gAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCt                                                               >  3:312252/1‑101 (MQ=255)
                                                                                                             gAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCt                                                               <  4:312252/101‑1 (MQ=255)
                                                                                                                         gCTTAGTAATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCGAGTCAGCCAGTCg               <  8:1267/137‑1 (MQ=255)
                                                                                                                           ttAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCGAGTCAGCCAGTCggg             >  7:31096/1‑137 (MQ=255)
                                                                                                                             aGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTCGCCATCTTGATAGCCTCGGCCGGGCACACCTCTGCGCTCTATATGGGTTCGAGTCAGCAAGTCGGGAg           >  2:141432/1‑137 (MQ=255)
                                                                                                                                cATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCGAGTCAGCCAg                  >  8:348000/1‑127 (MQ=255)
                                                                                                                                cATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCGAGTCAGCCAg                  <  7:348000/127‑1 (MQ=255)
                                                                                                                                cATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCGAGTCAGCCAGTCGGGAGTgg        >  8:15724/1‑137 (MQ=255)
                                                                                                                                cATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCGAGTCAGCCAGTCGGGAGTgg        >  8:42658/1‑137 (MQ=255)
                                                                                                                                      aaGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCGAGTCAGCCAGTCGGGAGTGGcaacaa  >  8:242841/1‑137 (MQ=255)
                                                                                                                                      aaGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCGAGTCAGCCAGTCGGGAGTGGcaacaa  >  3:334971/1‑137 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGG‑ATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCGAGTCAGCCAGTCGGGAGTGGCAACAA  >  ACB122/3365103‑3365372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑