Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB122 | 5,688,637 | 0 | A | C | 36.1% | 44.6 / 12.4 | 61 | S236R (AGC→CGC) | pyrC' | Dihydroorotase‑like protein |
Reads supporting (aligned to +/- strand): ref base A (15/24); new base C (6/16); total (21/40) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.16e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
TGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGC > ACB122/5688503‑5688769 | tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCCACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGCTCACCacc > 4:291155/1‑135 (MQ=255) tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGGCCGTCAGCCAGATCACCCgc > 4:36676/1‑137 (MQ=255) tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAgc > 8:301761/1‑137 (MQ=255) tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCGCTTAACCCCGATCCCCacc > 2:365787/1‑135 (MQ=255) ggCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCACCCTGCTGCTGGTGGAACAGAGCGGCGTGCGGTCGCACTTCAGCCAGATAACCCgcc > 4:54002/1‑136 (MQ=255) gCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGTAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAgcgc < 6:151326/137‑1 (MQ=255) ccATGGCCAGCTTCCTCGGCCTGCCGGGCTGCCCGGAGAGCGCCCAAACCGGGGCCCTGGCGCGCAACCTGCTGCTGGTGCAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCgg < 4:127413/137‑1 (MQ=255) ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGCGCGCGTCAGCCAGATCACCCGCGCCCGTGGCGCGCgg > 1:302984/1‑137 (MQ=255) cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGc > 2:21824/1‑137 (MQ=255) cATGCCCAGGTTCCTCGGCCGGCCGGGCAACCCGCACAGCGCGGAAACGGTGGCCCTGCCGCGCAACCTCCTGGTGGTGGAACAGAGCGGCGTGGGGGCGCAGTTCCGCCACATCCCCCGCGCCCGTGGCGCGCGGc < 4:311417/137‑1 (MQ=255) aTGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGGGGCCCGGGCGCGCAACCTGCTGCGGGGGCAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCt < 6:276205/137‑1 (MQ=255) ccAGCTTCCTCGGCCTGCCGGGCAGCCCGGAGACCGCCGAAACCGGGGCCCTGGCGCGCAACCTGCTGCTGGGGGACCAGAGCGGCGTGCGTGCGCACTTCAGCCCGATCCCCAGCGCCCGGGGCGCGCGGCTGATc < 7:77401/137‑1 (MQ=255) caaGCTCCCTCGGCGTGCGTGGCATCCGGGATAGCGCCCAAACGGTGCCCCTGGCGCGAAACGTGGTGCTGGGGTACCAGAGGGGCGTGCGTGCCCACTGCACCCAGACCACCCGCGCCCGTGGCGCGCGGCTGATc < 8:126685/135‑1 (MQ=255) aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGGCCTTGAGCCAGATCACCCCCGCCCGTGGCGCGCGGCGGCTCgc > 3:172900/1‑137 (MQ=255) cTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGCGCCCTTCAGCCCGATAACCAGCGCCCGTGGCGCGCGGCTGATCgccc > 5:306499/1‑137 (MQ=255) gccTCGGCGTGCCGGGAATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccag < 2:67550/136‑1 (MQ=255) cccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCGGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccag < 3:289693/136‑1 (MQ=255) ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg < 7:301761/137‑1 (MQ=255) gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGCGCACGTGCGCCCGATAACCAGCGCCCCGGGCGCGCGGCTGCTcgcccacgcccac > 1:20409/1‑136 (MQ=255) gggCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGCTAACCCGCCGCCGTGGCGCGCGGCTGGTGGCCCCGGCCCCGGCCGTgg > 8:200888/1‑137 (MQ=255) agagCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGCGCGGCGTGCGGGGGCACTTGCGCCAGCTCCCCAGCGCCCGGGGGGCGCGGCGGGTAGCCCCGGCCCCGGCCGGGGGCCGGGCGGtg > 3:169330/1‑136 (MQ=255) cgcgcCTAACCCGTGGCCCGGGCGCGCAACCTGCGGCTGGTGCAACAGAGCGGCGGGCGTGCGCACTGCACCCATATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 3:94851/136‑1 (MQ=255) cgcgcCGAAACGGGGGCCCTGGCGCGCACCCTGCGGCTGGTGCAACAGAGGGGGGTGCGTGCGCAGTTCAGCCACATCACCCGCGCCCTTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 1:210002/136‑1 (MQ=255) cgcgcCGAAACCGTGGCCGGGGCGCGCAACCTGCGGCGGGTGGAACAGAGCGGCGGGCGTGCGCACTTCCCCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 7:1584/136‑1 (MQ=255) agCGCCTATCCCGGGGCCCTGGCGGGCAACGTGGTGGTGGTGGAACAGCGCGGGGGGCGTGCGCACTTCAGCAAGAGCCCCCGGGCCCGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 1:52920/137‑1 (MQ=255) cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCt < 3:87887/96‑1 (MQ=255) cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCt > 4:87887/1‑96 (MQ=255) cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGCAACAGAGGGGCGGGCGGGCGCACTTCAGCCAGATCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg < 7:129519/137‑1 (MQ=255) aaaCCGTGGCCCTGGCGCGCACCCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCTCTTCAGCCAGATCACCAGCGCCCCTGGCGCGCGGCTGATCGCCCAGGCCCCGGCCTTGGGCCTGCCGGTTACCGCCGAt > 5:292868/1‑137 (MQ=255) ccggTGGCCCGGGCGCGCAACCTGCTGCTGGGGGAACAGCGCGGCGGGGGGGCGCACTGCAGCCCCATCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGt < 1:253011/134‑1 (MQ=255) cgggggCCCTGGCGCGCAACCTGCGGCTGGTGCAACAGCGCGGCGTGCGGGCGCACTTCCGCCAGACCCCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 1:196892/133‑1 (MQ=255) cgggggCCCTGGCGCCAAACGTGCGGCTGGGTCACCCGAGGGGGGGGGGTGCGAACTTCACCCACAGCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 3:6031/133‑1 (MQ=255) ccGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGACCAGAGCGGCGGGCGGGCGCAATTCCGCCAGATCCCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 4:73816/137‑1 (MQ=255) ccGTGGCCCTGGCGCGCAACCTGCTGCGGGGGCAACAGCGCGGGGGGCGTGCGCACTGCCGCCACATCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 4:128714/137‑1 (MQ=255) ccGTGGCCCGGGCGCGCACCCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCCCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 2:76517/137‑1 (MQ=255) ggcgcAACCTGCTGCTGGTGCAACAGAGGGGCGGGCGTGCGCACTTCACCCATATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg < 3:291155/136‑1 (MQ=255) cgcgcAACCTGCTGCGGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGAGCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg < 5:359116/137‑1 (MQ=255) cgcAACGTGCTGCTGGTGTAACCGAGGGGCGGGCGGGCGCACTTCCGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCt < 8:313147/137‑1 (MQ=255) cttgctgctgGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCCCGCCCAGGCCGTGGGCCGGCCGGTTACCGCCGCTGTAGCGCGGTACCAGCTGATcc > 2:16297/3‑137 (MQ=255) ccTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACATCAGCCAGAACACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCCGGCCTTGGGCCTGCCGGGAACCGCCGGTGTAGAGCGGTCCCAGCTGATcc > 4:279880/1‑137 (MQ=255) gAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTc > 7:235348/1‑137 (MQ=255) gAACAGAGCGGCGTGCGTGCGCACATCAGCCAGCTCACCCGCCCCCGTGGCGCGCGGCTGATCGCCCAGGCCCCGGCCTTGGGCCTGCCCGGAACCGCCGATGTAGCGCTGTACCAGCTGATCCGCACGGACGAggc > 8:256984/1‑135 (MQ=255) cgcggcgTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 4:316486/136‑1 (MQ=255) cgcggcgTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 2:20409/136‑1 (MQ=255) cgcggcgTGCGGGCGCACTTCAGCCCGACCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 5:113279/136‑1 (MQ=255) cgcggcgTGCGGGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 7:290529/136‑1 (MQ=255) cgcggcgGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 4:151720/136‑1 (MQ=255) gcggcgTGGGTGCGCACTTCAGCCAGATCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc < 1:16297/137‑1 (MQ=255) gcggcgTGCGTGCGCACTTCAGCCAGATCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc < 4:158768/137‑1 (MQ=255) ggtgcgtgcGCACTTCAGCCAGATCCCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 5:94864/136‑1 (MQ=255) ggtgcgtgcGCACTGCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 7:82961/136‑1 (MQ=255) cgtgcgtgCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCACGCCCAGGCCTTGGGCCTGCCGGTAAACGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt > 2:98758/1‑137 (MQ=255) cgggcgtgcgCACTTCACCAAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 6:105693/134‑1 (MQ=255) gcgtgcgCCCATAAGCCACATCACCACCCCCCCTGGCGCGCGGCTGATCGCCCCCGCCCACGCCTTGGGCCAGCCCGTAAACGCCGATGTAGCGCGGTAACACCAGATACAAACCGACGAGTCGCTGCGCGAGttat > 4:47393/1‑135 (MQ=255) gtgcgCACTTCCGCCAGATCCCCAGGGCCCGTGGCTCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc < 6:372899/137‑1 (MQ=255) gtgcgCACTTCAGCCAGAGCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc < 3:22752/137‑1 (MQ=255) gggcgcACTTCAGCCAGAGCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc < 3:36676/135‑1 (MQ=255) ggcccaaCTCCACCCAGCACACGACCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTATCCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc < 2:165585/131‑1 (MQ=255) aGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCa < 5:251476/137‑1 (MQ=255) cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCATCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCatc > 7:521/1‑135 (MQ=255) cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTCATCCTCACGGACGAGTCGCTGCGCGAGTTATCCAGCCTCTACCACGagcagc > 1:135393/1‑137 (MQ=255) ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTg > 7:362351/1‑104 (MQ=255) ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTg < 8:362351/104‑1 (MQ=255) | TGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGC > ACB122/5688503‑5688769 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |