| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 2,043,578 | 0 | A | C | 55.6% | 3.0 / 13.8 | 19 | intergenic (‑14/+161) | smrA/CJ019_01939 | putative DNA endonuclease SmrA/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (2/6); new base C (0/10); total (2/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGCCGGCCAGCTTCTGGCGGTCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGGATGTCACAGAGCCCGAAGACGGC > ACB143/2043448‑2043679 | cAGGCCGGCCAGCTTCTGGCGGTCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAggcg < 1:309240/135‑1 (MQ=255)cAGGCCGGCCAGATTCTGGCGGTCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAggcg < 6:385226/135‑1 (MQ=255) ctggttCTGGGGGTGGGCGTTGGGCTTGCCGACTTCGGCACGGGGGGGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGTAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGGGGCTTAGttt < 2:230408/131‑1 (MQ=255) cAGCTTGTGGCGGGCGGCGTTGGGCTTGCCGACTTCGGCACGGTGGGGGGGGATCGGCTTACCGCCGCGCACTTCGGCCTTCAACAGGTAAAAATCGGCGTCTTGCATGAGGCCCCCCCGTCGGCGGCGTAGtta < 2:275133/135‑2 (MQ=255) ctggtGGGGGTCGGCCTTGGGCTGGCCTACTTCGGCAGGGTGGTGGCGGATCGGCTTTCCGCCGCGCACTGCGGCCTTGAACAGGGAAAAATCGTCTTCTTGCATGTTGCCTCCACGTCGGCGGCGTTGTTTAcg < 8:81434/131‑1 (MQ=255) cTTCTGGCGGGCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTCAACCGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTCGGCGGCGTAGTTTAcg < 6:336478/135‑1 (MQ=255) cGGGCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGTAAAAATCGGCGTCTTGCATGATGCCTCCACGTCGGCGGCGTAGTTTCCGCGACtgtg < 4:96535/136‑1 (MQ=255) ggcGGCTTTGGGCTGGCGGACTTCGGCAGGGGGGGGGGGGTGGGGCTTTACGCCGCGCACTTCGGCCTTTAACCGGTAAAACTCGTGGTCTTGCATGTTGCCTCCCGTTCGGGGGGTTTGTTAACGCGACTGTgg < 8:155894/133‑1 (MQ=255) gCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTACGCGACTGTGGGGCg > 5:80688/1‑135 (MQ=255) gCTTGCCGACTTCGGCACGGTCGGGGCGGAGCGGCTTTACGCCGCGCACTTCGGCCTGGAACAGGCAAAAATCGTCGTCTTGCATGATGCCCCCACGTAGGCGGCGTAGTTTACGCGACTGTGGGGCGGTGTGc < 2:70277/134‑1 (MQ=255) aggggggTGGCGGTGGGGGTTTACGCCGCGCACTTGGGCATTGAACTGGTAAAAATGGTCGTCTTGCATGATGCCCCCCCGTCGGGGGCTTAGTTTCCGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCctct < 8:155320/129‑1 (MQ=255) aggggcGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGCAAAAATCGTCGTCTTGCATGATGCCTCCACGTCGGCGGCGTTGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCctct < 8:226246/130‑1 (MQ=255) aCGGTCGGGGCGTAGCGGCTTTACGCGGGGCAGTTGGGCCTTGAAGTGGTAAAATTTGGCGTCTTGCATGATCCCGCCACGTGGGGGGGTTTTTTTCGGCGACTGTGGGGCGGTGTGCTGCCTGTGCGGGCctct < 2:1944/135‑1 (MQ=255) gTCGTGGCGGTTCGGCTTAACGCCGCGAACTGCGGCCTTGAACAGGTAAAAATCGTCTTCTTGCATGATGCCTCCACGTAGGCGGCTTAGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTcgc < 4:345170/136‑1 (MQ=255) gtcgcGGTTTTCCGCCGCGCACTGCGCCCTTCAACAGGAAAAACTCGGCGGCTGGCAGGAGGCCCCCACGTCGGGGGGGTTTTTACCGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAAc < 4:221897/132‑1 (MQ=255) gATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAggcg < 5:129475/75‑1 (MQ=255) gATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAggcg > 6:129475/1‑75 (MQ=255) aCTTCGGCCTTCAACAGGAAAAAATCGTCGTCTTGCAGGATGCCTCCACGTCGGCGGCGTAGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGGATGTc < 1:259262/135‑1 (MQ=255) aaaaaaTCGGCGTCTTCCATGATCCCCCCCCGTCGGGGGGGTTTTTACCGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGGATGTCACAGAGCCCGAAGAcggc < 6:80688/134‑1 (MQ=25) | CAGGCCGGCCAGCTTCTGGCGGTCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGGATGTCACAGAGCCCGAAGACGGC > ACB143/2043448‑2043679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |