Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,737,4430TC36.6% 49.3 / 19.6 41I229I (ATT→ATC) paoBAldehyde oxidoreductase FAD‑binding subunitPaoB
Reads supporting (aligned to +/- strand):  ref base T (21/5);  new base C (0/15);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 2.44e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCGGCATTCCATCGCCTCCCCGAGGACACCCCGGAGCGAGACAATCAGCTGGCAATCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCGAGACCCGGT  >  ACB143/3737317‑3737571
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tGCGGCATTCCATCGCCTCCCCGAGGACACCCCGGAGCGAGACAATCAGCTGGCAATCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACcg                                                                                                                          >  2:223675/1‑135 (MQ=255)
  cGGCATTCCATCGCCTCCCCGAGGACACCCCGGAGCGAGACAATCAGCTGGCAATCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACcgcg                                                                                                                        >  7:156182/1‑135 (MQ=255)
  cGGCATTCCATCGCCTCCCCGAGGACACCCCGGAGCGAGACAATCAGCTGGCAATCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACcgcg                                                                                                                        >  6:347195/1‑135 (MQ=255)
      aTTCCATCGCCACCCCGATCACACCCCGCCGCGACACATTCCGCGGGCACTCGAGGACGTGGTCCGCCCCATCGCGCTGCCCCCCCCGGTGTTCTAAAAAAATAACCGTTATCTCAAAATTCGCGACCGCGcctc                                                                                                                    <  6:340061/135‑1 (MQ=255)
          cATCGCCCCCCCGAGGACACCCCGGAGCGAGAAAATCCGCTGGCAATCGATGAACTGGTGGGCGCCATCGAGCTGCCGCCCCCGGTGTTCTAAAAACATTACCGTTATCTTAAAATTCGCGACCGCGCCTCCTAcg                                                                                                               <  3:111982/136‑1 (MQ=255)
                ccccccGAGCACCCCCCGGCCCCAGAAAAGCCGCTGCCATCCGCTGACGGGGTGGCCCCAAGCGCGCGGCCGCCCCGGGTGTCCAAAAAAAATACCCTTTATCTAAAAATCCGCGACCGCGCCTCCTACGCGttt                                                                                                          <  6:112920/133‑1 (MQ=255)
                  ccccGAGGACACCCCGGAGCGAGACAATCAGCTGGCAATCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTgc                                                                                                        >  8:48433/1‑135 (MQ=255)
                                cgccgCGAGACAATCAGCTGCAAAGCCAGCACGTGGTGGGCGCCACCGAGCGCCCGCCCCCGGTGTCCGAAAAAAATTACCGTTATCTCAAAATCCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTcg                                                                                          <  7:79748/131‑1 (MQ=255)
                                cGGAGCGAGACAATCAGCTGGCAATCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTcg                                                                                          >  5:155338/1‑135 (MQ=255)
                                 ggAGCGAGACAATCAGCTGGCAATCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCTCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTcgc                                                                                         >  6:115245/1‑135 (MQ=255)
                                     cGAGACAATCAGCTGGCAATCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAg                                                                                    >  4:78895/1‑136 (MQ=255)
                                        gaCAATCAGCTGGCAATCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCt                                                                                 >  3:373143/1‑136 (MQ=255)
                                           aaTCAGCTGGCAATCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCt                                                                                 >  5:311528/1‑133 (MQ=255)
                                           aaTCAGCTGGCAATCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCt                                                                                 >  5:299058/1‑133 (MQ=255)
                                           aaTCAGCTGGCAATCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCGCCTACGCGTTTGCGCTGGTATCGGTCGCAGCAGCCt                                                                                 >  8:137974/1‑133 (MQ=255)
                                              cAGCTGCAAATCTATGATGTGGTGGCCGCGTTCGCGCGGCCGCCCCGGGTGTTCAAAAAAAATAAGTTTTATCATAAAAACCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCt                                                                           <  4:199999/136‑1 (MQ=255)
                                                gCTGGCAATCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCtt                                                                          >  1:89967/1‑135 (MQ=255)
                                                    gCAATCGATGAACTGGTGGTCGCCATCGAGATGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCTCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGAt                                                                      >  7:350065/1‑135 (MQ=255)
                                                       aTCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATgg                                                                    >  7:277265/1‑134 (MQ=255)
                                                                         gCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTc                                                >  3:351537/1‑136 (MQ=255)
                                                                           cATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCg                                               >  5:202270/1‑135 (MQ=255)
                                                                              cGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCt                                            >  2:352865/1‑135 (MQ=255)
                                                                                        cccccGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGCCGCTGggc                                  >  5:330252/1‑135 (MQ=255)
                                                                                        cccccGGTGTTCCAAAAACATAACCGTTATCTCAAAATCCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGggcg                                 <  4:373143/136‑1 (MQ=255)
                                                                                          ccgggTTTTCAAAAAAATTTCCCTTTACCTAAAAACCCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGggcgg                                <  5:49716/132‑1 (MQ=255)
                                                                                                  ccaaaaaaaATAACCGTTATCTCAAAATCCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCt                         <  6:303742/131‑1 (MQ=255)
                                                                                                          gaTTACTGTTATATCAAAATCCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCg                <  2:144957/134‑1 (MQ=255)
                                                                                                            ttccctttATCTTAAAATCCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTg              <  5:186674/129‑1 (MQ=255)
                                                                                                               cctttATCTAAAAATCCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCg           <  6:297373/132‑1 (MQ=255)
                                                                                                               ccgtattCTTAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCg           <  1:29251/129‑1 (MQ=255)
                                                                                                               ccGTTATCTCAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCg           <  6:392458/135‑1 (MQ=255)
                                                                                                                 tttATCTAAAACTCCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCgag         <  5:156283/134‑1 (MQ=255)
                                                                                                                 ttaaTCTGAAAATCCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCgag         <  1:328319/132‑1 (MQ=255)
                                                                                                                 gTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCgag         <  8:57379/135‑1 (MQ=255)
                                                                                                                 gTTATCTCAAAATCCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCgag         <  2:217297/135‑1 (MQ=255)
                                                                                                                       tGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCGAGACCCgg   >  8:374383/1‑135 (MQ=255)
                                                                                                                       tGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCGAGACCCGGt  >  4:141238/1‑136 (MQ=255)
                                                                                                                        gAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCGAGACCCGGt  >  8:144868/1‑135 (MQ=255)
                                                                                                                         aaaaTCCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCGAGACCCgg   <  4:330559/133‑1 (MQ=255)
                                                                                                                         aaaaTCCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCGAGACCCGGt  <  4:322518/134‑1 (MQ=255)
                                                                                                                         aaaaTCCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCGAGACCCGGt  <  8:277265/134‑1 (MQ=255)
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TGCGGCATTCCATCGCCTCCCCGAGGACACCCCGGAGCGAGACAATCAGCTGGCAATCGATGAACTGGTGGTCGCCATCGAGCTGCCGCCCCCGGTGTTCGAAAAACATTACCGTTATCTGAAAATTCGCGACCGCGCCTCCTACGCGTTTGCGCTGGTATCGGTCGCCGCAGCCTTGAGCTTCGATGGCCAGGTGATCCGGCAAGCTCGCCTGGCGCTGGGCGGCGTTGCTCACAAGCCGTGGCGAGACCCGGT  >  ACB143/3737317‑3737571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑