Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,135,5900AC40.4% 22.0 / 10.9 52Q32P (CAG→CCG) tgnC(Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (15/16);  new base C (18/3);  total (33/19)
Fisher's exact test for biased strand distribution p-value = 8.27e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTCTG  >  ACB143/4135455‑4135723
                                                                                                                                       |                                                                                                                                     
cAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCa                                                                                                                                       <  3:355058/136‑1 (MQ=255)
 aaGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCa                                                                                                                                       <  5:20090/135‑1 (MQ=255)
      cGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACCGCCTCAACCCGGCCACCCCCCCGGcca                                                                                                                                  >  8:7602/1‑134 (MQ=255)
           ccTCACCGAATCCGCCAACGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCACGCCTGGgcg                                                                                                                             >  2:318301/1‑135 (MQ=255)
           ccTCACCGAAGCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCCACAGCCTCAACCCGGCCACCGCCCAGGCCTGGgcg                                                                                                                             <  7:156244/135‑1 (MQ=255)
                ccGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGTCAAGACCTTCGACAGCCTCAACCCGGCCCCCGCCCAGGCCCGGGCGCAAca                                                                                                                        >  8:326585/1‑134 (MQ=255)
                 cGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGTCAGCCTCAACCCCGCCCCCCCCCCGCCCACGGCGCCACTg                                                                                                                       >  6:249524/1‑135 (MQ=255)
                 cGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCAcag                                                                                                                       >  1:137661/1‑133 (MQ=255)
                 cGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCAcag                                                                                                                       >  1:371443/1‑133 (MQ=255)
                  gAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGCCACAGc                                                                                                                      >  1:217315/1‑135 (MQ=255)
                    aTCCGCCAAGGGGTTTGTTGCATGACCCTGGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCg                                                                                                                   <  4:306384/136‑1 (MQ=255)
                        gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCACCGGCCCGAcc                                                                                                                >  1:403227/1‑134 (MQ=255)
                          cAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGCAACTGCCCgccgccc                                                                                                             >  3:230509/1‑135 (MQ=255)
                            aGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCCACCCGGGCACCGCCCCCGCCGGGGCGCCACAGCCCgccgccca                                                                                                            >  7:214351/1‑133 (MQ=255)
                            aGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCAGGGCCCCCCAGCCCGACGccc                                                                                                             >  3:141272/1‑133 (MQ=255)
                            aGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCCACTGCccgccgccgc                                                                                                            >  8:111233/1‑134 (MQ=255)
                              gggATTGTTGCAGGACCCTCGTTCGTTTCCAGATGTGCAGCGACGGCAAATGGCGCGATGCCCAGAGCGCCAACACCTTCGACAGCCTCAACCCGGCCCCCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGa                                                                                                         <  3:316789/136‑1 (MQ=255)
                               ggATTGTTGCATGACCCTCGTTCGTTTCCAGATGTCCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTGCGACAGCCTCAACCCGCCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGa                                                                                                         <  1:41047/135‑1 (MQ=255)
                                             cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCAGGGCGCCACAGCCCGACCCCCATGAAGCCGATGTGGAGCt                                                                                          >  4:367485/1‑136 (MQ=255)
                                             cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCACGCCAGGGCGCCACGGCCCGACGCCGATGAAGCCGATGTGGAGCt                                                                                          >  4:3912/1‑136 (MQ=255)
                                                   ttcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCg                                                                                     >  2:12438/1‑135 (MQ=255)
                                                       tttCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCa                                                                                 <  6:269192/135‑1 (MQ=255)
                                                        ttCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGttgcg                                                                                >  2:384459/1‑132 (MQ=255)
                                                           cAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCAGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCAGGCCCTTAAGgcc                                                                             >  1:222618/1‑135 (MQ=255)
                                                           cAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGGGCAACTGCCCGACCCCCAAGAAGCCGCTGTGGGGCTGGGCGGTAAGgcc                                                                             >  6:155508/1‑135 (MQ=255)
                                                               tgtgCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACAGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGccc                                                                         >  2:150552/1‑135 (MQ=255)
                                                               tgtgCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCAGGGCGCCACAGCCCGCCCCCCCGGAAGCCGCTGTGGAGCTGGGCGTTCAAGCCGcccc                                                                        >  4:190329/1‑135 (MQ=255)
                                                                 tgCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCAACTGCCCGCTGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCCGc                                                                      >  3:91217/1‑136 (MQ=255)
                                                                   cagccccGGCAAATGCCGCGATGCCCAGAGCGGAAACACCTTCGACCCCCTCACCCCGGCCACCCCCCAGGCCTGTGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGc                                                                      <  5:9746/128‑1 (MQ=255)
                                                                    aTCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACAGCCCGCCGCCGCTGAAGCCGATGTGGGGCTGGCCGGGGAGGCCGCCCcgcggg                                                                   >  3:372711/1‑134 (MQ=255)
                                                                      cGACGGCCAATGGCGCGATGCCCCGAGCGGCAAGACCTTCGCCAGCCTCAACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGc                                                                  <  2:103090/135‑1 (MQ=255)
                                                                      cGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGGGCAACTGCCCGACGCCGCTGAAGCCGTTGTGGAGCTGGGCGTTGAGGCCGCCCCCCGTGc                                                                  >  1:388755/1‑135 (MQ=255)
                                                                         cGGCCAAGGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCtt                                                               <  1:322795/135‑1 (MQ=255)
                                                                           gCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCTGGGCGCCACTGCCCGACGCCGCTGAAGCCGATGTGGCGCTGGCCGGTCAGGCCGCCCCGCGGGccggcg                                                             >  1:274913/1‑131 (MQ=255)
                                                                              aagggCGCGAGGCCCAGAGCGGCAAGACCTTCGACACCCTCACCCCGCCCCCCCCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACa                                                          <  5:272433/132‑1 (MQ=255)
                                                                                  gggctaTGCACAGAGCGGCACGACCTTCGACAGCCACAACCCGCCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAg                                                     <  4:31589/131‑1 (MQ=255)
                                                                                       aTGCCCAGAGCGGCAAGACCTTCCACAGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg                                                <  3:139593/136‑1 (MQ=255)
                                                                                         gCCCAGAGCGGTAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTgg                                               >  7:112302/1‑135 (MQ=255)
                                                                                         gCCCAGAGCGGCAACCCCAGCCACAGCCTCACCCCCCCCACCGCCCAGGCCTGGCCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg                                                <  3:315287/134‑1 (MQ=255)
                                                                                             agagCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcag                                          <  4:230509/136‑1 (MQ=255)
                                                                                                 cGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCCAGGCGTGGCgcagca                                        >  7:310789/1‑134 (MQ=255)
                                                                                                       gACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCCGCGTGCCGGAGACAGCAAGGCGTGGCGCAGCCTCAccc                                  >  6:291843/1‑133 (MQ=255)
                                                                                                       gACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACCGCAAGGCGTGGCGCAGCATAAccc                                  >  6:297523/1‑133 (MQ=255)
                                                                                                       gACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCAGCCCAGCGTGCCGTAGCCATCCAGGCGTTGCGCCGCATCCCCg                                  >  2:38670/1‑134 (MQ=255)
                                                                                                       gACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTAAGGCCGCCCAGCGTGCCTTACACAGCAAGGCGTGGCGCCGCAACAccc                                  >  7:230688/1‑133 (MQ=255)
                                                                                                               aCAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACAGCCCCACGCCGCTGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTGGACAGCGAGGCCGGGCGCACCCAAACCGCTAccccccg                        >  4:348848/1‑136 (MQ=255)
                                                                                                                  gCCTCACCCCGGCCCCCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg                      <  1:191249/135‑1 (MQ=255)
                                                                                                                  gCCTCAACCCGCCCACCGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg                      <  1:234040/135‑1 (MQ=255)
                                                                                                                        aCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCaa                  <  6:92720/133‑1 (MQ=255)
                                                                                                                         cccGCCCACCCCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctg              <  4:170100/136‑1 (MQ=255)
                                                                                                                                aCCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGcgt        <  6:1467/135‑1 (MQ=255)
                                                                                                                                aCCGCCCAGGCCAGGGCGCAACTGCCCGACGCCGATGAAGCCCATGTGGAGCTGGCCGTTCAGGCCGCCCACCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCAACCGCCCCCGGCCCGCGGCAGcgtc       >  3:243874/1‑136 (MQ=255)
                                                                                                                                 ccGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCCGCATCACCGCTACCGCCCGCGGCAAGCTGCGGcgtc       >  2:134137/1‑135 (MQ=255)
                                                                                                                                      cAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTCTg  <  5:271282/135‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
CAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTCTG  >  ACB143/4135455‑4135723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑