Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,425,0700GC37.5% 37.0 / 20.1 32intergenic (‑29/‑89)CJ019_04200/CJ019_04201hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (15/5);  new base C (0/12);  total (15/17)
Fisher's exact test for biased strand distribution p-value = 2.94e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCC  >  ACB143/4424935‑4425185
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tgCGAGTCGAGCTGCGCAGTCGGCTTGCCGACACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCTTCAGCCCCGCAGCCACCGGCAGAATCGCCCGCAGGTACCCTCCTCTGGGTCGCAAAAaagaag                                                                                                                     >  4:227053/1‑136 (MQ=255)
       cGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGCAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGActc                                                                                                              >  4:384150/1‑136 (MQ=255)
        gAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGAct                                                                                                               >  8:67464/1‑134 (MQ=255)
        gAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGAct                                                                                                               >  8:231441/1‑134 (MQ=255)
                 aGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCCCAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCAGGTTCCATCCTCGGGGTGGCATAAAAGAAGGCGACTCTAGCACTg                                                                                                      >  1:305219/1‑134 (MQ=255)
                      gCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAATAAGGCGACTCTAGCACTggcaggc                                                                                                >  6:340584/1‑135 (MQ=255)
                      gCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTggcaggc                                                                                                >  7:68245/1‑135 (MQ=255)
                             gccACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGa                                                                                         >  6:193823/1‑135 (MQ=255)
                               accgcgCTGTTCCCTGGGTGCCAGACCCACACCGGGCCACCATCCCCACCGCCGCCAACGCCATAAACGCAGGCATGGTCCAGCCTCGGGGTCCAAAAAACCACCGCGACTCTAGCACTGGCAGGCATCCCGAGt                                                                                       <  2:58541/133‑1 (MQ=255)
                                aCGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGt                                                                                       >  6:88438/1‑134 (MQ=255)
                                aCGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGt                                                                                       >  6:274839/1‑134 (MQ=255)
                                                                      gcaaccgcaccgcaccCAACGCCATAATCGCACGCATGTTCCATCCGCGGGGCCCAAAAAAACACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATg                                                <  1:98118/129‑1 (MQ=255)
                                                                       cATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATg                                                >  8:346716/1‑134 (MQ=255)
                                                                                 ccAGCCAACGCCAGAATCCCAGGATTGTCCCATCCGGGGGGTCGAAAAAAACACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGa                                    <  3:56537/135‑1 (MQ=255)
                                                                                  cAGCCACCGTCAGAATCGCACTCATGTACCATCCTGGGGGTCGAAAAAAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGa                                    <  8:94861/135‑1 (MQ=255)
                                                                                  cAGCCAACGCAAGAATCCCATGCATGTACAATCCTCGGGGCTCAAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGa                                    <  1:359260/135‑1 (MQ=255)
                                                                                     ccAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAAcc                                 >  3:279590/1‑135 (MQ=255)
                                                                                     caaaCGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTATAGCACTGGCAGGAAACCCGAGTGAATTGCAGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAAc                                  >  5:185352/3‑134 (MQ=255)
                                                                                           gCAGAATCGACCCCATGTAGCATCCACGTGGTGGAAAAAAAGAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATg                          <  4:234199/136‑1 (MQ=255)
                                                                                            cataaTCCCACGCATGTTCCATCCTCGGGGTCCAAAAAAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATg                          <  1:60312/132‑1 (MQ=255)
                                                                                            cataaTCCCACGCATGTTCCATCCTCGGGGTCCAAAAAAACACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATg                          <  2:341446/132‑1 (MQ=255)
                                                                                             aGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATg                          >  2:125978/1‑134 (MQ=255)
                                                                                                  cgcacgaaTGTCCAATCCTCGGGGTCAAAAAAAACACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTg                    <  5:92858/135‑1 (MQ=255)
                                                                                                   gcacgcaTGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGc                   <  7:231441/135‑1 (MQ=255)
                                                                                                     tggattGTCCCATCCTGGGGGGCTAAAAAAATCTCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCAc                 <  8:68245/130‑1 (MQ=255)
                                                                                                      cgcaTGTACCATCCTCGGGGTCTAAAAAAACACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGc                   <  7:67464/132‑1 (MQ=255)
                                                                                                       gcaTGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTAt              >  4:253566/1‑136 (MQ=255)
                                                                                                                  cgtCGGTGCCGCAAAAAACACGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcg    <  4:244292/133‑1 (MQ=255)
                                                                                                                    tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgcc  >  8:220126/1‑135 (MQ=255)
                                                                                                                    tCGGGGTCCAAAAAAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgcc  <  5:280621/135‑1 (MQ=255)
                                                                                                                    tCGGGGGCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgcc  <  5:376322/135‑1 (MQ=255)
                                                                                                                    tCGGGGGCGCAAAAAAGAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgcc  <  5:275060/135‑1 (MQ=255)
                                                                                                                    tCGGGGCTGAAAAAACCAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgcc  <  8:185239/135‑1 (MQ=255)
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TGCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCC  >  ACB143/4424935‑4425185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑