Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,693,1750AC45.1% 13.1 / 15.3 54S236R (AGC→CGC) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base A (13/15);  new base C (9/14);  total (22/32)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑TGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAG  >  ACB143/5693056‑5693306
                                                                                                                        |                                                                                                                                   
ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGG‑GGGGCCCGTCAGCCACATCACCCCCGCCCCGGgcgccc                                                                                                                      >  1:180726/1‑133 (MQ=255)
ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑TGCGCCCTTCAGCCAGAAAACCAGCGCCCGTGgcgcgc                                                                                                                      >  8:18594/1‑135 (MQ=255)
ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑GGCGCACTTAAGCCCGATCACCAGCGCCCCTGgcgcgc                                                                                                                      >  1:308446/1‑135 (MQ=255)
   gggCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGCCCCGGGCGCGCAACCTGCTGCTGGTGGACCAGAGCGGGGGGCG‑GGCGCACTTCAGCCAGATCCCCCGCGCCCGTGGCGCGCGGc                                                                                                                   <  7:9938/134‑1 (MQ=255)
        aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCTAAACCGGGGCCCGGGCGCGCAACCTGCTGCTGGGGCAACAGAGCGGCGTGCG‑GGCGCACTTCCGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                              <  5:87805/135‑1 (MQ=255)
        aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGG‑TGGGCCCGTGCGCCCGATCACCCGCGCCCGTGGCGCGCGGCTGCTc                                                                                                              >  1:77676/1‑135 (MQ=255)
        aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑TGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                              >  5:166201/1‑135 (MQ=255)
        aGCTTCCGCGCCCGGCCGGGCATCCCGGAGCGCGCCGAAACCGTGGCCCTGGCGCGCACCCTGCTGCTGGTGGAACAGAGCGGGGTGGG‑GGCGCACTGCAGCCAGACCACCCCCGCCCGTGGCGCGCGGCTGATc                                                                                                              <  5:330722/135‑1 (MQ=255)
         gCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑TGCGCACTTCAGCCAGATCACCAGCGCTCGTGGCGCGCGGCTGATCg                                                                                                             <  2:381766/135‑1 (MQ=255)
             ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑TGGGCCCTTCAGCCCGATCCCCCGCGCCCGTGGCGCGCGGCTGATCgcccc                                                                                                         >  6:358550/1‑134 (MQ=255)
              cTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑TGCGCACTTCAGCCAGATCACCAGCGCCCGTAGCGCGCGGCTGATCgcccagg                                                                                                       <  4:267555/136‑1 (MQ=255)
               tCGGCCTGCGGGGCATCCCGGAGAGCGCCGAAAGCGGGGCCCTGGCGCGCAACCTGCTGCTGTTGGAACAGTGGGGCGTGGG‑TGCGATTTTCAGCCAGATCACCCGCGCCGGTGGCGCGCGGCTGATCgcccagg                                                                                                       <  2:39593/135‑1 (MQ=255)
               tCGGCCTGCCGGGCATCCCGGAGAGCGCGGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGCGCGGCGTGCG‑TGCGCACTTCAGCCAGATCCCCCGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                       <  6:177672/135‑1 (MQ=255)
               gcGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGGGCAACAGAGCGGCGTGCG‑TGCGCACTTCCGCCACATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                       <  8:302051/134‑1 (MQ=255)
                cggccggcGGGGCATCCCGCTGACCGCCCAAACGGGGGCCCTGGCGCGCAACCTGCTGCTGGGGAACCAGGGGGGGGGGCG‑TGCGCACTTCACCCAGAACCCCCGGGCCCGTGCCGCGCGGCTGATCgcccaggc                                                                                                      <  2:60531/135‑1 (MQ=255)
                  gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑GGCGCACTTAAGCCACATCACCACCGCCCGTGGCCCGCGGCTGATCgcccaggccc                                                                                                    >  7:120980/1‑135 (MQ=255)
                                  ggAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGG‑GGGGGCCTTGCGCCAGCTCCCCCGCGCCCGGGGCGCGCGGGGGGTCGCCCCGGCCCCCGGCGTGGGccgggc                                                                                    >  5:297003/1‑131 (MQ=255)
                                  ggAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑TGGGCCCTTCCGCCCGATCCCCCGCGCCCGTGGCGCGCGGCTGCTCGCCCCGGCCCAGGCCGTGGGCCTGcc                                                                                    >  2:30839/1‑135 (MQ=255)
                                  ggAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑GGCGCACTTAAGCCAGCACACCAGCGCCCGGGGCGCGCGGCTGAACGCCCAGGCCCAGGCCTGGGGCCTGcc                                                                                    >  2:252232/1‑135 (MQ=255)
                                       gcgcCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCG‑TGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                              <  4:357185/136‑1 (MQ=255)
                                        cgcCGAAACCGGGCCCCGGGCGCGCACCCTGCTGCGGGTGCAACAGAGCGGCGTGCG‑GGCGCACTTCAGCCACACCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                              <  5:90240/135‑1 (MQ=255)
                                           cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGG‑TGGGCCCTTGAGCCCGATAACCAGCCCCCGTGGCGCGCGGGGGGTAGCCCCCGCCCCCGCCGGGGGCCGGCCGGTAAccgcc                                                                          >  3:119282/1‑136 (MQ=255)
                                           cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGG‑GGGGCCCTTAAGCCCGATCACCCCCGCCCGGGGCGCGCCGCGGCTAGCCCACGCCCCGGCCGTGGGCCAGGCCGTAAccgc                                                                           >  6:262812/1‑135 (MQ=255)
                                               acggTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACATAGCGGCGGGCG‑TGCGCACTTCAGCCAGATCACCTGCGCGGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATg                                                                      <  4:38793/133‑1 (MQ=255)
                                                 cggggCCCTGGCGCGCAACCTGCTGCGGGGGGAACAGAGCGGCGGGCG‑GGCGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                    <  4:318418/133‑1 (MQ=255)
                                                  ggggCCCGGGCGCGAAACGTGGTGCTGGTGGACCAGAGCGG‑GTGGGTTGCGCAGTCCAGCATGACCCCCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                    <  2:174989/133‑1 (MQ=255)
                                                  gTGGCCGTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑TGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                    <  8:303485/135‑1 (MQ=255)
                                                  gTGGCCCTGGCGCGCAACCTGGTGGGGGGGCAACAGCGCGGGGTGGG‑GGCGCAGTTCCGCCACATCACCCGCGCCCGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                    <  8:87990/135‑1 (MQ=255)
                                                  gTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑TGCGCACTTCCGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                    <  1:29385/135‑1 (MQ=255)
                                                  gTGGCCCTGGCGCGCAACCTGCGGCTGGTGGAACAGAGCGGGGTGCG‑TGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                    <  7:204818/135‑1 (MQ=255)
                                                     gCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑TGGGCACATCAGCCCGAACACCAGCGCCCGTGGCGCGCGGCGGATCGCCCCGGCCCCGGCCGTGGGCCTGCCGGGAACCGCCGATGTAgcg                                                                 >  6:337167/1‑135 (MQ=255)
                                                      cccTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGG‑TGGGCCCTTCAGCCCGATAACCCGCGCCCGGGGCGCGCGGCGGATAGCCCACGCCCAGGCCTTGGGGCGGCCGGTTACCGCCGATGTAgcgc                                                                >  6:131167/1‑135 (MQ=255)
                                                       ccTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGG‑TGCGCACATCACCCACATAACCACCGCCCCTGGCCCGCCGCTGATAGCCCCCGCCCACGCCGTGGGCCGGCCCGTAAACGCCGATGTAGCCCt                                                               >  2:125028/1‑135 (MQ=255)
                                                       ccTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑TGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCt                                                               <  8:114464/135‑1 (MQ=255)
                                                            cgcgcAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑TGCGGACTTCAGCCCGATCCCCCGCGCCCGTGGCGCGCCGCTGCTCGCCCCGGCCCCCGCCGTGGGCCTGCCGGTAACCGCCGCTGTTGCGCTgga                                                            >  1:226151/1‑131 (MQ=255)
                                                             gcgcAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑TGCGCACTTCAGCCCGATAACCCGCGCCCGTGGCGCGCGGCTGATCGCCCCGGCCCCCGCCGTGGGCCTGCCGGTAACCGCCGAGGTAGCGCTGTAccc                                                         >  8:228253/1‑134 (MQ=255)
                                                              cgcAACCTGCTGCTGGGGGAACAGAGCGGCGTGCG‑TGCGCACTTCAGCCATATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg                                                        <  6:295132/135‑1 (MQ=255)
                                                                  aCCTGCTGCGGGTGGAACACAGCGGGGGGCG‑TGCGCACTTCAGCCTCATCCCCAGCGCCCGTGGGGCGCGGCGGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGAt                                                   <  4:290830/136‑1 (MQ=255)
                                                                                  aCAGAGCGGCGTGCG‑TGCGCACTTCAGCCAGATCCCCAGCGCCCGTGGCGCGCGGCGGATCGCCCCGGCCCCGGCCGTGGGCCTGCCGGTAAACGCCGATGTAGCGCTGTACCAGCTGATCCGCCCGGAAGACTCg                                   >  3:280764/1‑136 (MQ=255)
                                                                                        cggcggGCG‑TGCGCACTCCGGCGACATCACCTCCGCCCGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                              <  2:118786/135‑1 (MQ=255)
                                                                                           cgtgcg‑tgCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCGTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGa                           >  7:104332/1‑135 (MQ=255)
                                                                                            gggcg‑ggcgCACTTCAGCCAGCCCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                         <  4:392447/134‑1 (MQ=255)
                                                                                             tgcg‑tgcgCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                         <  5:166611/135‑1 (MQ=255)
                                                                                             tgcg‑tgcgCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCTAGt                         <  8:108844/135‑1 (MQ=255)
                                                                                             tgcg‑ggcgCATTACAGCCATATCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                         <  8:328253/135‑1 (MQ=255)
                                                                                             tgcg‑ggccCACTTCCCCCAGAGCCCCCGCGCCCGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                         <  5:340562/135‑1 (MQ=255)
                                                                                             gggg‑tgccCAGTTCCCCCCGACCACCTGCGCCCGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                         <  2:85149/132‑1 (MQ=255)
                                                                                              ggggggcgcACTGCACCCAGAGCACCACCGCCCGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                         <  8:304008/130‑1 (MQ=255)
                                                                                              gcg‑tgcgCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt                        <  1:51643/135‑1 (MQ=255)
                                                                                              gcg‑tgcgCACATAAGCCAGATAACCACCGCCCCTGGCGCGCGGCTGATCGCCCCGGCCCAGGCCTTGGGCCAGCCGGTAACCGCCGATGTAGCGCGGTTCCAGCTGAACCTCACGGACGAGTCGCTGCGCGAGtt                        >  8:275598/1‑135 (MQ=255)
                                                                                                   gcgcACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                    <  7:228253/135‑1 (MQ=255)
                                                                                                    cgcACTTCAGCCAGATAACCAGCGCCCGTGGCGCGCGGCTGATAGCCCAGGCCCACGCCTTTGGCCTGCCGGTAACCGCCGATGTAGCGCGGTACCAGCTGATCCTCCCGGACCAGTCGCTGCGCCAGTTCTCCa                   >  8:180425/1‑135 (MQ=255)
                                                                                                                    cACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATATAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg  <  4:75618/136‑1 (MQ=255)
                                                                                                                     aCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg  >  6:348239/1‑135 (MQ=255)
                                                                                                                        |                                                                                                                                   
CCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCG‑TGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAG  >  ACB143/5693056‑5693306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑