Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,721,8750GC28.9% 74.1 / 12.0 45G96G (GGC→GGG) CJ019_01617hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/5);  new base C (1/12);  total (28/17)
Fisher's exact test for biased strand distribution p-value = 2.41e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAA  >  ACB143/1721741‑1722010
                                                                                                                                      |                                                                                                                                       
tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGCCCCAccc                                                                                                                                       >  6:15790/1‑137 (MQ=255)
tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGCCCCAGcc                                                                                                                                       >  5:211073/1‑137 (MQ=255)
tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCCGcc                                                                                                                                       >  1:109999/1‑137 (MQ=255)
tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCCGcc                                                                                                                                       >  1:114471/1‑137 (MQ=255)
tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGcc                                                                                                                                       >  7:266638/1‑137 (MQ=255)
tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGcc                                                                                                                                       >  8:271962/1‑137 (MQ=255)
tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGcc                                                                                                                                       >  3:265878/1‑137 (MQ=255)
tCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTACTCGACCCAGcc                                                                                                                                       >  4:242915/1‑137 (MQ=255)
      gTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCgg                                                                                                                                 >  7:226747/1‑137 (MQ=255)
      gTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACCCCAGGTGATTTCCTCTTCACCGTCGGCGCTTATGCGGACCCAGCGGTCAGCAAATTCTTCCCCGTATTACGAGTACCACCCACCCgg                                                                                                                                 >  1:103870/1‑137 (MQ=255)
          aCCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTACTCGACCCAGCCACCCGgcgcg                                                                                                                             >  5:335078/1‑137 (MQ=255)
              ataccTACTGAAACTACCACGCCTGCCTTTTCATTATGTACGCCTGGTTATTTCCTCTTCCCGGGCGGCGCTGATGCGGACCCCGCGGGACGCTTTTTCTCCCCCGTATTCCTCGCCCCAGCCCCCCGGCgcgcagc                                                                                                                         <  6:244841/134‑1 (MQ=255)
                   tACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCCCCCGGCGCGCAGCGCAca                                                                                                                    >  7:17127/1‑136 (MQ=255)
                                aaGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAgcc                                                                                                       >  3:129119/1‑136 (MQ=255)
                                  gCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCCGCCACCCGGCGCGCAGCGCACTTCCACACCCAGcgcg                                                                                                     >  8:278440/1‑137 (MQ=255)
                                     tGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCCCACCCAGCCCGGcc                                                                                                  >  5:246188/1‑136 (MQ=255)
                                                gatgTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGcgcg                                                                                       >  7:193802/1‑137 (MQ=255)
                                                    tACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGgcg                                                                                   >  6:252468/1‑137 (MQ=255)
                                                              gATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTGAGCATCTTCTTCCCCGTCTTCCTAGACCAAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGAGGCGAGTGCGCAGGCAACGt                                                                         >  3:166419/1‑137 (MQ=255)
                                                              gATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCCCCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGt                                                                         >  2:31304/1‑137 (MQ=255)
                                                               aTTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCCGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTc                                                                        >  5:122565/1‑137 (MQ=255)
                                                               aTTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTc                                                                        >  1:184572/1‑137 (MQ=255)
                                                                       ttCACCGTCGGCGCTTATGCGGCCCCAGCGGTAAGAATCTTCTCCCCCGTCTACCTCCACCCCCCCCCCCGGCGCGCCGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGccc                                                                <  4:304444/137‑1 (MQ=255)
                                                                               cGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCCGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCggc                                                        >  4:226814/1‑135 (MQ=255)
                                                                                   gcTGTTGCGCCCCCCGCGGTCAGAATGTTCTTCCCCGTATCCCCCCACCCCCCCCCCGGGCGCGCCGCCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGt                                                    <  2:114471/137‑1 (MQ=255)
                                                                                   gcTGATGCGGACCCAGGGGTCAGCATCTTATTCCCCGTCTCCCGCGACCCCCCCCCCCGGCGCGCCGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGt                                                    <  4:265878/137‑1 (MQ=255)
                                                                                   gcTGATGCGCACCCCGCGGTCAGAATCTTCTTCCCCGTCTTCCTCCCCCCACCCCCCCGGCGCGCCGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGt                                                    <  8:271456/137‑1 (MQ=255)
                                                                                       ttGCGGACCCTGCGGTCAGCATGTTCTACCCCGTCTTCCTCCACCCCGCCACCCGGCGCGCCGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCt                                                <  6:358465/136‑1 (MQ=255)
                                                                                       aggcgcACCCAGCGGTCATCATCTTCTGCCCCGTCTTCCTCCCCCCACCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCt                                                <  6:69111/135‑1 (MQ=255)
                                                                                        ggcgcACCCCGCGGTCAGCATCTTCTGCCCGGTCTTCCTCGCCCCCGCCCCCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCtt                                               <  5:151759/136‑1 (MQ=255)
                                                                                                 cccGGTCACCATATTATCCCCCGTCTCCCTCGCCCCCCCCCCCCGGCGCCCCGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATAc                                      <  8:107704/135‑1 (MQ=255)
                                                                                                    ggTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAgg                                   >  4:242147/1‑137 (MQ=255)
                                                                                                         gCATGTTCTTCCCCGTCTTCCCCCACCCCCCCCCCCGGCGCGCCGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGtt                              <  4:338734/137‑1 (MQ=255)
                                                                                                          aaTCTTCTTCCCCGTCTACCCCGACCCACCCCCCCGGCGCCCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTc                             <  3:72173/136‑1 (MQ=255)
                                                                                                          aaTCTTCTACCCCGTCTGCCGCGCCCCCCCCCCCCGGCGCGCAGCCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTc                             <  4:156871/136‑1 (MQ=255)
                                                                                                            tcttctTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCa                           >  8:257331/1‑137 (MQ=255)
                                                                                                            tcttatTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTACACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACCTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTcaa                           >  6:328523/1‑135 (MQ=255)
                                                                                                                 ttcCCCGTCTACCGCCACCCACCCCCCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTcc                      <  5:52614/137‑1 (MQ=255)
                                                                                                                 tgccccGGCTTCCCCGACCCCCCCCCCGGGCGCGCACCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTcc                      <  6:224536/135‑1 (MQ=255)
                                                                                                                       gTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTACCTACaa                >  5:224536/1‑136 (MQ=255)
                                                                                                                        tCTTCCTCGCCCCAGCCCCCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGc               <  8:285856/137‑1 (MQ=255)
                                                                                                                          ttCCTCCCCCCAGCCCCCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCt             <  1:31304/137‑1 (MQ=255)
                                                                                                                           tCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCtt            >  2:16784/1‑137 (MQ=255)
                                                                                                                             cTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTgg          >  1:8002/1‑137 (MQ=255)
                                                                                                                                   cccgCCCCCCGGCGCGCCGCCCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAAcc    <  6:184758/134‑1 (MQ=255)
                                                                                                                                   ccACCCCCCCGGCGCGCCGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAAcc    <  7:271962/137‑1 (MQ=255)
                                                                                                                                     aGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCaa  >  8:347871/1‑137 (MQ=255)
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TCGCGGGTAAACCCATTCCTACAGAAACGAGCAAGCCTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGCGGGCGCAGGCAACGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAA  >  ACB143/1721741‑1722010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑