Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	2,348,251	0	A	C	25.4%	142.3 / 12.7	72	V266G (GTG→GGG)	oprJ_1	Outer membrane protein OprJ
Reads supporting (aligned to +/- strand):  ref base A (9/44);  new base C (18/0);  total (28/44)
Fisher's exact test for biased strand distribution p-value = 1.51e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTCTCTAGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAGTGGCCA  >  ACB143/2348116‑2348380                                                                                                                                         |                                                                                                                                  gTCTCTAGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCAc                                                                                                                                  <  8:73006/137‑1 (MQ=255)    tctAGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGGCG‑CGGCcccccc                                                                                                                               >  5:210737/1‑135 (MQ=255)    tctAGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCG‑CGGCCAcccc                                                                                                                               >  2:76414/1‑135 (MQ=255)      tAGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAgg                                                                                                                             <  5:224023/137‑1 (MQ=255)      tAGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAgg                                                                                                                             <  5:346801/137‑1 (MQ=255)      tAGGGTGGCCGCCTGCAGTTCCCGTTCGGACTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAgg                                                                                                                             <  5:85756/137‑1 (MQ=255)       aGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGGCG‑CGGCCAccccggg                                                                                                                            >  1:246334/1‑132 (MQ=255)         ggTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCTCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCA‑CCGCCACCGCgggcg                                                                                                                          >  4:319685/1‑134 (MQ=255)         ggTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGGCG‑CGGCCCCCCCGGccg                                                                                                                          >  1:302869/1‑136 (MQ=255)         ggTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCCCCCCGGccg                                                                                                                          >  4:191389/1‑136 (MQ=255)            ggCCGCCTGCAGTTCCCGTTCGGCCTGTCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGc                                                                                                                       <  1:269161/137‑1 (MQ=255)            ggCCGCCTGCAGTTCCCGTTCGGCCTGGCGCCCAGCTGGCCGGCGGTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGAGGGCTACGCAGGTCGGGGCCT‑CGGCCACCGAGGCCTGGc                                                                                                                       <  8:161778/137‑1 (MQ=255)            ggCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGc                                                                                                                       <  5:215496/137‑1 (MQ=255)            ggCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAACGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGc                                                                                                                       <  8:208066/137‑1 (MQ=255)               cgccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGGCG‑CGGCCACCGCGGCCGGGCCAc                                                                                                                    >  3:169493/1‑137 (MQ=255)               cgccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCGG‑CGGCCCCCGCGGGCGGGcccc                                                                                                                    >  2:338115/1‑135 (MQ=255)               cgccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCG‑AGGCCCCCCCGGCCTGGGCAc                                                                                                                    >  5:148104/1‑137 (MQ=255)                gccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCCCCGAGGCCTGGCCAcc                                                                                                                   >  5:163879/1‑136 (MQ=255)                gccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCAca                                                                                                                   >  8:172460/1‑136 (MQ=255)                gccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CCGCCCCCGCGGCCGGGcccca                                                                                                                   >  3:260094/1‑134 (MQ=255)                gccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCG‑AGGCCCCCCCGGCCGGGcccca                                                                                                                   >  7:21617/1‑134 (MQ=255)                gccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCA‑CGGGCCCCGCGGGCTGGccccc                                                                                                                   >  4:51406/1‑134 (MQ=255)                gccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCA‑CGGCCCCCCCGGCCAGGccccc                                                                                                                   >  6:78490/1‑134 (MQ=255)                gccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCA‑CGCCCCCCGACGCCGGGCCAca                                                                                                                   >  5:158254/1‑136 (MQ=255)                 ccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGGCG‑ACGCCACCGCGGGCAGGcccatg                                                                                                                  >  6:120737/1‑133 (MQ=255)                 ccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCAc                                                                                                                                  <  4:131659/121‑1 (MQ=255)                 ccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTg                                                                                                                  <  1:143312/137‑1 (MQ=255)                 ccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCG‑CCGCCCCCCCGGCCTGGccccag                                                                                                                  >  1:28651/1‑133 (MQ=255)                 ccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCG‑CCGCCCCCCCGGCCGGGccccgg                                                                                                                  >  7:194959/1‑133 (MQ=255)                 ccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGCCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTCGCCTACGGCCGCC‑AGGCCGGGGCaagg                                                                                                                  >  4:256893/1‑134 (MQ=255)                   tGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCCCCCCGGCCGGGccccatcg                                                                                                                >  7:200553/1‑131 (MQ=255)                       gTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATg                                                                                                            <  5:119532/137‑1 (MQ=255)                             gTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCCCCGAGGCCTGGCCCCAGAGCCTGCTCAGt                                                                                                      >  5:116554/1‑137 (MQ=255)                             gTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGt                                                                                                      >  2:17263/1‑137 (MQ=255)                                   ccTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCCAGGCCGGGCCACAGAGCAAGGTCAGTTAGTaa                                                                                                >  8:86184/1‑137 (MQ=255)                                     tGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCA‑CGGCCCCCGAGGCCTGGCCCCTGAGCATGGTCACTTAGTaaaa                                                                                              >  8:78339/1‑136 (MQ=255)                                              tCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTg                                                                                     <  4:277556/137‑1 (MQ=255)                                                tGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTgcc                                                                                   <  1:12542/137‑1 (MQ=255)                                                tGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTgcc                                                                                   <  4:171757/137‑1 (MQ=255)                                                      gCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCggg                                                                             <  8:194948/137‑1 (MQ=255)                                                          tCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCCCCGAGGCCTGGCCACGGAGCATGCTCAGTTCGTAAACCGCCATATGCCGCCGGGCGga                                                                         >  6:147276/1‑137 (MQ=255)                                                                aaaTGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCAt                                                                   <  6:161003/137‑1 (MQ=255)                                                                aaaTGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCA‑CGGCCACCGCCGCCGGGCCACTGCGGCTTCTCAAGTTGGAAAGCGCCCTGTGCCCCCGGGCGGAGTGcca                                                                   >  8:333459/1‑135 (MQ=255)                                                                     ccagccaTCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCa                                                              <  2:37493/137‑1 (MQ=255)                                                                          caTCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTgc                                                         <  1:83310/137‑1 (MQ=255)                                                                                 aGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCCCCCCGGCCTGGCCCCGGAGCCTGCTCAGGTCGGAAAGCGCCATCTGCCGCCGGGCGGGGAGCCTCGGCAGTTGggcccgcc                                                  >  6:96750/1‑136 (MQ=255)                                                                                          cGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTccc                                         <  1:81225/137‑1 (MQ=255)                                                                                               ccGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAg                                    <  5:96750/137‑1 (MQ=255)                                                                                                      cGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCcagca                             <  4:130560/137‑1 (MQ=255)                                                                                                      cGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCAACTTTTACAGCAAGCCcagca                             <  3:317329/137‑1 (MQ=255)                                                                                                         ggggATGGCTACGCAGGTCGTGGGCG‑CGGCCCCCCCGGCCGGGCCCCAGGCGCTGCGCAGTTGGTTAAGCGCCCAAAGACGCCGGGGGGTGAGCATAGGCAGTTGGGCCCCCACCGGTTCACGCCAACCCAGCAggg                          >  4:115978/1‑136 (MQ=255)                                                                                                           ggATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGgcgc                        <  7:86184/137‑1 (MQ=255)                                                                                                           ggATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGgcgc                        <  7:172460/137‑1 (MQ=255)                                                                                                           ggATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGgcgc                        <  5:177717/137‑1 (MQ=255)                                                                                                           ggATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGgcgc                        <  7:30361/137‑1 (MQ=255)                                                                                                           ggATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGgcgc                        <  5:78490/137‑1 (MQ=255)                                                                                                           ggATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGgcgc                        <  4:33277/137‑1 (MQ=255)                                                                                                                gCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGcgctcgct                   <  5:147276/137‑1 (MQ=255)                                                                                                                  tACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGt                 <  8:33956/137‑1 (MQ=255)                                                                                                                      cAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAg             <  1:268973/137‑1 (MQ=255)                                                                                                                       aGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAgg            <  2:145919/137‑1 (MQ=255)                                                                                                                       aGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAgg            <  3:81731/137‑1 (MQ=255)                                                                                                                       aGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAgg            <  4:288714/137‑1 (MQ=255)                                                                                                                       aGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAgg            <  5:35419/137‑1 (MQ=255)                                                                                                                       aGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAgg            <  5:342977/137‑1 (MQ=255)                                                                                                                                ccT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAGTGGcc   <  7:28475/137‑1 (MQ=255)                                                                                                                                 cT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAGTGGCca  <  1:76414/137‑1 (MQ=255)                                                                                                                                 cT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAGTGGCca  <  1:310938/137‑1 (MQ=255)                                                                                                                                 cT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAGTGGCca  <  3:173673/137‑1 (MQ=255)                                                                                                                                 cT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAGTGGCca  <  4:276216/137‑1 (MQ=255)                                                                                                                                 cT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAGTGGCca  <  3:191389/137‑1 (MQ=255)                                                                                                                                 cT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAGTGGCca  <  1:209414/137‑1 (MQ=255)                                                                                                                                 cT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAGTGGCca  <  5:331911/137‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  GTCTCTAGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCT‑CGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAGTGGCCA  >  ACB143/2348116‑2348380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑