Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,273,8780CA30.9% 60.4 / 11.1 42D40E (GAC→GAA) fabG_53‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG
Reads supporting (aligned to +/- strand):  ref base C (16/13);  new base A (0/13);  total (16/26)
Fisher's exact test for biased strand distribution p-value = 4.65e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACCAATGGAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAG  >  ACB143/3273743‑3273999
                                                                                                                                        |                                                                                                                         
aCCAATGGAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCTTAACCCGTCGCCAGGCCGACa                                                                                                                          >  5:184385/1‑137 (MQ=255)
               caTGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCgctgc                                                                                                           >  6:273034/1‑137 (MQ=255)
               caTGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCgctgc                                                                                                           <  2:61967/137‑1 (MQ=255)
                          aacaaAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATa                                                                                                >  8:8288/1‑137 (MQ=255)
                          aacaaAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATa                                                                                                >  6:136685/1‑137 (MQ=255)
                          aacaaAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATa                                                                                                >  3:44091/1‑137 (MQ=255)
                                       gCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGGCGCCCGCCCGAAAAACTCCCCCCGCGGCTGGAGTAAATACACGCCGCTggcg                                                                                   <  2:210928/137‑1 (MQ=255)
                                         tGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTggcggc                                                                                 >  7:352214/1‑137 (MQ=255)
                                         tGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTggcggc                                                                                 >  6:237459/1‑137 (MQ=255)
                                         tGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTggcggc                                                                                 >  5:100655/1‑137 (MQ=255)
                                               gcGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGGCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGcc                                                                           <  1:226645/136‑1 (MQ=255)
                                                  gggcgggtGACGCCACTGTCGGCTATATCGGCAAGGTTTTTGCCGCG‑GAGGGCTATATTCCGTGCGTAACCCGTAGCAACGCCGAAAAACTGCAGCCGCTGCGGGAGGAAATACACGCCGCTGGCGGCCAGGCCTAc                                                                        <  8:343283/137‑1 (MQ=255)
                                                    gggggcgACGCCAGTGGCGGCTATAGCGCCAAGGGTTTTG‑CGGGTGAGGGCTATATGGCCTGCTTACCCCGGCGCCAGGCCGAAAAACTGCAGCCGCTGCTGGAGTAAAACCACGCCGCTGGCGGCCAGGCCTACgg                                                                      <  4:121205/135‑1 (MQ=255)
                                                                aCTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAATCTGCAGCCGCTGGTGGAGGAAATACACGCCGCTGGCGTCCAGGCCTACGGTTTTGGCTCTgc                                                          >  7:189143/1‑136 (MQ=255)
                                                                aCTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCCTCCGCTGCTGGAGGAAATACACTCCGCTGGCGGCAAGGCCTACGGTTTTGGCTATGa                                                          >  8:5438/1‑137 (MQ=255)
                                                                 gggggggcgAGACCGCCAAGGGTTTTG‑CGCGTGAGGGATATATTGCCTGGTAAACCCGTCGCCCGCCCGAAAAACTGCCGCCGCTGCTGTAGTAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGAt                                                         <  6:187588/132‑1 (MQ=255)
                                                                   ggcggcGAGATCGCCAAGGGTTTTG‑CGCGTGCGGGCAATATGGCCTGCGTAACCCGGCGCCAGGCCGACAACCTCCAGCCGCTGCTGGAGGAAAACCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATgc                                                       <  4:95524/137‑1 (MQ=255)
                                                                      ggcgATAGCGCATAGCTTTTGG‑CGGGTTTGGGCTATATTGCGTGGTTAACCCGGCGCCTGGCCGACAAACTGCCGCCGCTGCGGGCGAAAATCCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATgcgcg                                                    <  6:71809/137‑1 (MQ=255)
                                                                      ggcgAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATgcgcg                                                    <  5:273034/137‑1 (MQ=255)
                                                                         gagaTCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCaa                                                 >  4:58979/1‑137 (MQ=255)
                                                                          agaTCGCCAAGCGTTTTG‑CGGGTGAGGGCTATATTGCGTGCGTACCCCGGCGCCAGGCCTACAAACTCCAGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAg                                                <  5:252848/137‑1 (MQ=255)
                                                                           taTCGCAAAGCTTTTTG‑CGGGTGAGGCCTATATTGCGTGGGTAACCCGGCGCCAGGCCGACAAACTGCAGCCGCTGCTGGTGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAgg                                               <  8:336018/136‑1 (MQ=255)
                                                                                         tgg‑cgcTTGTGGGATATATTGCCTGCTAAACCGGTCGCACGGCAGAAAAACGCCCGCGGGGGCGGCACTAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                 <  5:9785/135‑1 (MQ=255)
                                                                                         tgg‑cgcGTGAGGGATATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCCGCCGCGGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                 <  8:196285/135‑1 (MQ=255)
                                                                                          tG‑CGCGTGAGGGATATATTGCCTGCGTAACCCGTCGCCAGGCCCAAAAACTGCAGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCg                                <  1:31118/137‑1 (MQ=255)
                                                                                               ggggAGGTCTATATTGCCTGCTTAACCCGCCGCCAGGCAGAAAAACTCCAGCCGCTGCGGCAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGGTTTGTCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACt                            <  2:23981/134‑1 (MQ=255)
                                                                                               ggTGCGGGCTATATTCCCGGCGTAACCCGGCGCCAGCCCTAAAAACTGCACCCGCGGCTGGAGTAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACt                            <  6:92500/136‑1 (MQ=255)
                                                                                               ggTGAGGGATATATTGCCGGGGAACCCCGTCGCCAGGCCTAAAAACTGCAGCCGCTGCTGGACTAAAACCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACt                            <  3:345138/136‑1 (MQ=255)
                                                                                                         atatTGCGTGCGAAACCGGGCCCCCGGCCGAAAAACTGCCGCCGCTGTTGTAGTAAAACCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACt                  <  5:55687/137‑1 (MQ=255)
                                                                                                            ttGCCTGCGTAACCCGGCGCCAGGCCTACAAACTGCAGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATc               <  6:132245/137‑1 (MQ=255)
                                                                                                            ttGCCTGCGTAACCCGGCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATc               <  2:16235/137‑1 (MQ=255)
                                                                                                            ttGCCTGCGAAACCCGTCGCCCGGCAGAAAAACGCCCGCCGCGGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGACGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATc               <  1:191646/137‑1 (MQ=255)
                                                                                                              gCCTGCTTAACCCGTGGCCAGGCCGACAAACTGCAGCCGCTGCTTGTGTAAATCCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGa             <  6:184385/137‑1 (MQ=255)
                                                                                                              gCCTGCGTAACCCGTCGCCAGGCCTAAAAACTGCAGCCGCTGCTGTAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGa             <  7:8288/137‑1 (MQ=255)
                                                                                                              gCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGa             <  5:136685/137‑1 (MQ=255)
                                                                                                                  gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGGGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGt         >  3:337400/1‑137 (MQ=255)
                                                                                                                  gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGt         >  7:5161/1‑137 (MQ=255)
                                                                                                                    gTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGa       >  3:90638/1‑137 (MQ=255)
                                                                                                                     tAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGat      >  1:57742/1‑137 (MQ=255)
                                                                                                                     tAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGat      >  8:299473/1‑137 (MQ=255)
                                                                                                                         ccGTCGCAAGGCCGAAAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAg  <  6:65053/137‑1 (MQ=255)
                                                                                                                         ccGGCGCCCGGCCGACAAAGTGACGCCGCTGCGGGCGCAAAAACACGCCGCGGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAg  <  8:147324/137‑1 (MQ=255)
                                                                                                                                        |                                                                                                                         
ACCAATGGAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTG‑CGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAG  >  ACB143/3273743‑3273999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑