Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,028,4510TA30.9% 63.3 / 14.4 42intergenic (‑120/‑127)CJ019_03802/mcpU_3hypothetical protein/Methyl‑accepting chemotaxis protein McpU
Reads supporting (aligned to +/- strand):  ref base T (22/7);  new base A (0/13);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 3.04e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCATGGTTGGCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGATCCGCCCCCATG  >  ACB143/4028328‑4028580
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tCATGGTTGGCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACgg                                                                                                                      >  1:337303/1‑137 (MQ=255)
     gTTGGCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAACTTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAggg                                                                                                                 >  5:292369/1‑137 (MQ=255)
     gTTGGCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAggg                                                                                                                 >  3:24083/1‑137 (MQ=255)
        ggCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGcc                                                                                                              >  3:10147/1‑137 (MQ=255)
             aCCTCATAACCACTCATAAAAGCTACAAGTCTGATGCAGTGGGCGGCTTATTTCAAAGGCTCTTTCTGGGGCCGCTTTAACGGCGTAAATATTAAATTTTATTTTTTTAAAAAAAAAAA‑AACGGCAGGGGCCGatat                                                                                                         <  4:17972/137‑1 (MQ=255)
                 cATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATtgtg                                                                                                     >  3:111016/1‑137 (MQ=255)
                   ttACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTggg                                                                                                   >  8:31212/1‑137 (MQ=255)
                     aCGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGTGGCCGATATTGTGGGAg                                                                                                 >  1:255113/1‑137 (MQ=255)
                     aCGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAg                                                                                                 >  7:62291/1‑137 (MQ=255)
                       gACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGc                                                                                               >  5:220028/1‑137 (MQ=255)
                       gACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGc                                                                                               >  3:271632/1‑137 (MQ=255)
                            aaaaaaGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGa                                                                                          >  1:145963/1‑137 (MQ=255)
                            aaaaaaGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGa                                                                                          >  5:181462/1‑137 (MQ=255)
                               aaaGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGt                                                                                       >  3:132986/1‑137 (MQ=255)
                                        aCTCTGACCCAGTGGGCGGCATATGTCAAACCCGCCTTGATGCGGCGCTTTAACGGCGCACAAATTTAAATTGAATTTTTCAAAAAAGAAAA‑CACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCg                                                                              <  7:183473/137‑1 (MQ=255)
                                        aCTCTGAAGCAGTGGGGGGCATATGCAAAAACCCCCTTGAGGCGGCGCTTTAACGGCGCAAAAATTAAAATTAAATGTTTCAAAAAACAAAA‑CACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCg                                                                              <  4:117468/137‑1 (MQ=255)
                                        aCTCGGACGCAGGGGGCGGCAGATGACAAACGCGCCTTGATGCGGCGCTTTAACGGCGTAAAAATTAAAATTGATTGTTTCAATAAACAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCg                                                                              <  7:146128/137‑1 (MQ=255)
                                                ccAGGGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGTACAAATTAAAATTGAATGTTTTAATAAACAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCtt                                                                      <  7:267823/136‑1 (MQ=255)
                                                 gaGTGGGCGTAATATGTAAAATCCGCATTTATGCGGCGCTTTAACGGAGTAAAATTTTAAATTGATTGTTTTAAAAAATAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCttt                                                                     <  4:211389/136‑1 (MQ=255)
                                                 cAGGGGGCGGCACATGTAAAACGCGCCTTGATGCGGCGCGTAAACGCCGGACAAATTTAAATTGAATTTTTTAATAAACAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCttt                                                                     <  6:171769/137‑1 (MQ=255)
                                                  tgTGGGCGGCAGTTGACAAAAGCGCCTTGAGGCGGCGCGTAAACGGCGGAAAAATTTAAATTGAATGTTTTAAAAAACAAAA‑CACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTg                                                                    <  8:62291/136‑1 (MQ=255)
                                                       gcggcAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCAcc                                                               >  4:138354/1‑137 (MQ=255)
                                                       gcggcAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCAcc                                                               >  8:328537/1‑137 (MQ=255)
                                                       gcggcAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AAAGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTTGCCGGAGCGCTTTGGCAcc                                                               >  1:187124/1‑137 (MQ=255)
                                                         ggcAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACctc                                                             >  8:101835/1‑137 (MQ=255)
                                                           cAGTTGTCAAACGCCCCGTGATGCGGCGCGTTAACGGCGTACAATTTAAAATTGATTTTTTGAAAAAAGAAAA‑CACGGCAGGGGCCGATATTGTGGGAGGCATCGAGGTGCCTGGACGGAGCGCTTTGGCACCtctc                                                           <  6:165833/137‑1 (MQ=255)
                                                           cAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCtctc                                                           >  7:72849/1‑137 (MQ=255)
                                                           cAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAAGTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCtctc                                                           >  8:35776/1‑137 (MQ=255)
                                                            tgATGTCAAATGCCCCTTCTGGCGTCGCGTTATCGGCGGACAAATTTAAATTCAATGTTTCAAAAAAGAAAA‑CCCGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCg                                                          <  7:226796/136‑1 (MQ=255)
                                                             tagctCAAACGCCCCTGGATGCGGCGCGTTACCGGCTTAAAAATTAAATTTGATTGTTTAAAAAAAAAAAC‑AACGGCCGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCgg                                                         <  4:10147/133‑1 (MQ=255)
                                                             taTGTCAAACGCGCCTTGAGGCGGCGCTTAAACGGCGCACAAATTAAAATTTAATGTTTCAAAAAAAAAAA‑CACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCgg                                                         <  7:40299/136‑1 (MQ=255)
                                                                        cgcTTTGAGGCGGCTTTTTAACGGCGAAAAAATTAAAATTAAATGTTACAAAAAAAAAAA‑CACGGCAGGGGCCAATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAg                                              <  5:29865/137‑1 (MQ=255)
                                                                         gcCTTGATGCGGCGCTTTAACGGCGGAAAAATTTAAATTTAATGTTTCAAAAAACAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGa                                             <  5:318489/137‑1 (MQ=255)
                                                                                       cgTAAACGGCTTAAAAATTAAAATTGAATTTTTGAATAAAAAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAAccc                               <  4:308407/137‑1 (MQ=255)
                                                                                        gTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCt                              >  5:287345/1‑137 (MQ=255)
                                                                                             cggcggACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTg                         >  3:189595/1‑137 (MQ=255)
                                                                                                 cgACAAATTAAAATTGACTGTTTTAAAAAACAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCaa                     <  6:210998/136‑1 (MQ=255)
                                                                                                        tttAAATTTAATGTTTCAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGa              <  6:158313/137‑1 (MQ=255)
                                                                                                        tttAAATTGAATTTTTTAATAAAAAAAA‑CACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGa              <  7:352227/137‑1 (MQ=255)
                                                                                                             aTTCAATGTTTTAAAAAATAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGATCCGc         <  4:339651/137‑1 (MQ=255)
                                                                                                                tctttttttAATAAAAAAAACCACGGCCGGGGCCGATATTGTGGGAGGCAGCGCGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGATCCGccc       <  2:347180/132‑1 (MQ=255)
                                                                                                                    gTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCCCCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGATCCGCCCCCATg  >  4:68879/1‑137 (MQ=255)
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TCATGGTTGGCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAA‑AACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGATCCGCCCCCATG  >  ACB143/4028328‑4028580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑