Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,425,0700GC36.7% 28.0 / 17.4 30intergenic (‑29/‑89)CJ019_04200/CJ019_04201hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (17/2);  new base C (0/11);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 1.43e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCTGCCTTGCCTGCCC  >  ACB143/4424935‑4425203
                                                                                                                                       |                                                                                                                                     
tgCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAgg                                                                                                                                      >  6:93794/1‑137 (MQ=255)
tgCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCAACCTCGGGGTCGCAAAAaacaagc                                                                                                                                      >  2:286702/1‑136 (MQ=255)
         aGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAg                                                                                                                             >  6:308893/1‑137 (MQ=255)
                 aGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTggca                                                                                                                     >  6:180139/1‑137 (MQ=255)
                 aGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTAGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATACTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCAATggca                                                                                                                     >  2:269512/1‑137 (MQ=255)
                           ccgccACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCCCCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGACGGCGACTCTAGCACTGGCAGGCAGCCCgt                                                                                                           >  6:214081/1‑136 (MQ=255)
                             cccACCCCCCTTTCCCATGGCCCCACAACCACACCCGCCCCGCACCCCCCCCCCCCCCACCGCCACAATCCCACCCATGTCCCACCCCCGGGGCCCAAAAAAACCCCGCGACTCTAGCACTGGCAGGCATCCCGAGt                                                                                                         <  4:61966/136‑1 (MQ=255)
                                aCGCGCCGTTTCCATGGTCGCAGCAGCACAACCGGCCAGAATCAGCACCGCAGCAAACGGCAGAATCGAACGCATGTTCCATCATCGGGGTCGCAAAAAAGAAGGCGACTCTAGCCCTGGCAGGCATCCCGAGTGaa                                                                                                      >  8:353325/1‑137 (MQ=255)
                                              tgggcccAGAACCACACCCGCCCAGCATCCCCCCCCCACCCAACGGCAGAATCCCACGCTTGTCCCATCCGCGGGGCCCAAAAAAACACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTg                                                                                        <  1:240495/131‑1 (MQ=255)
                                                    caaacgCACCACCCGCCAGCATCAGCACCCCACCCACCGCCAAAATCCCACGCAGTTTCCATCCTCGGGGTCGAAAAAAACACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTg                                                                                  <  5:111975/132‑1 (MQ=255)
                                                       aaGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCg                                                                               >  6:161680/1‑137 (MQ=255)
                                                                     aGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGc                                                                 >  4:324645/1‑137 (MQ=255)
                                                                           cgCACCTCACCCAACGGCATATGCTCACGCATTTTCAATCCTCGGGGTCTCAAAAAATAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCAc                                                           <  8:34764/136‑1 (MQ=255)
                                                                              cccGCAGCCAAGGGCAGAATCGCCCTCATTTTCAATCCGGGGGGTCGAAAAAAAGACGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAg                                                        <  5:295907/136‑1 (MQ=255)
                                                                                cccatccAACGGAATAATCGCACGCTTGTTCCATCCTGGGGGTCTAAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGa                                                      <  7:192013/132‑1 (MQ=255)
                                                                                    gCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGTGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAAccc                                                  >  8:145954/1‑137 (MQ=255)
                                                                                     ccAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCca                                                 >  7:114508/1‑137 (MQ=255)
                                                                                          gccAGAACCGCACGCTTGTGCCATCCTCGGGGTCGAAAACAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATg                                            <  4:237479/135‑1 (MQ=255)
                                                                                               aagcgcccgctTGTTCAATCCGCGGGGCCGCAAAAAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt                                       <  1:24698/134‑1 (MQ=255)
                                                                                                 ccgcccgcaTGTCCCCTCCGGGGGGTCCCAAAAACCACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGc                                     <  3:141613/136‑1 (MQ=255)
                                                                                                       gcaTTTTCCAACGTGGGGGTCTAAAAAACCACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATc                               <  3:255641/137‑1 (MQ=255)
                                                                                                       gcaTGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATc                               >  5:362142/1‑137 (MQ=255)
                                                                                                       gcaTGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATc                               >  6:336507/1‑137 (MQ=255)
                                                                                                                 acgtCGGGGACTCAAAAACCAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgc                     <  1:49368/134‑1 (MQ=255)
                                                                                                                        ggTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtg              >  2:306338/1‑137 (MQ=255)
                                                                                                                         gTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtgc             >  4:248210/1‑137 (MQ=255)
                                                                                                                         gTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtgc             >  4:110055/1‑137 (MQ=255)
                                                                                                                            aaaaaaaaCACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtgcctt          <  5:266935/135‑1 (MQ=255)
                                                                                                                              aaaacaGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtgccttgc        <  2:265384/137‑1 (MQ=255)
                                                                                                                              aaaaaaGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCCCTATCTGCTACTCGCCGCCCtgccttgc        >  3:55493/1‑137 (MQ=255)
                                                                                                                              aaaaaaGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtgccttgc        >  4:194099/1‑137 (MQ=255)
                                                                                                                              aaaaaaGAAGGCGACTCTAGCACTGGCAGGCATCCCGACTGAATTGCAGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTCCACTATCAGCTACTCGCCGCCCtcccttgc        >  4:198744/1‑137 (MQ=255)
                                                                                                                                    gaagGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCTGCCTTGCCTGccc  <  8:114508/137‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
TGCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCTGCCTTGCCTGCCC  >  ACB143/4424935‑4425203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑