Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,383,7580CG35.3% 46.0 / 10.2 34intergenic (+528/‑330)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (4/18);  new base G (12/0);  total (16/18)
Fisher's exact test for biased strand distribution p-value = 3.32e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTG  >  ACB143/5383626‑5383893
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gCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCtttt                                                                                                                                      <  5:263868/137‑1 (MQ=255)
gCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCACCTGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGtttt                                                                                                                                      >  6:100258/1‑137 (MQ=255)
         gcggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTtttggcg                                                                                                                             >  4:146496/1‑134 (MQ=255)
          cggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTTTTTtgtggggg                                                                                                                            >  6:286791/1‑133 (MQ=255)
                      aaaTCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCACCCGGCTCCACCAAATCCAAAGAAGAAGACCCCCTCGCGCCTGGTTTTTTTTTACAGGAATTTgggggg                                                                                                                >  8:10543/1‑134 (MQ=255)
                                   cGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCGCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGaaaa                                                                                                   <  1:133017/137‑1 (MQ=255)
                                         aGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGa                                                                                            <  5:232608/137‑1 (MQ=255)
                                          gCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTGTGGCGGGCTTTTTGGGGCGGGGGGGGGAAAAAT‑AGGAt                                                                                           >  2:100025/1‑137 (MQ=255)
                                           cATGTAGTACCGCCAGCGGTGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGAtt                                                                                          <  1:113623/137‑1 (MQ=255)
                                              ttagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTaa                                                                                       <  1:15776/136‑1 (MQ=255)
                                                agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAAcc                                                                                     <  3:8035/137‑1 (MQ=255)
                                                agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAAcc                                                                                     <  7:273470/137‑1 (MQ=255)
                                                 gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTGTGGGTGGAATTTTGGGGCGGCGGGTCGAAAAAA‑CGGTTTTAAccc                                                                                    >  2:34599/1‑137 (MQ=255)
                                                 gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTGTTTTTTTTCCTGGAATTTGGGGGGGGGGGGTCGTAAAAG‑CGGATTTAAacc                                                                                    >  4:210165/1‑137 (MQ=255)
                                                          gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTGTGGCGGGGATTTGGGGGCTGCGGGGGGGAAAATAGGGATTTAACCCCttgttgt                                                                            >  1:254320/1‑132 (MQ=255)
                                                             gAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTTTTTTGTGGCTGGAATTTTGGGGCTGCGGGGCGAAAAAT‑CGGATTTAACCCCATTATTGTAgg                                                                        >  6:86160/1‑137 (MQ=255)
                                                                tACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAACCCCTTTCTTGTAGGGCg                                                                     <  6:324554/137‑1 (MQ=255)
                                                                   tGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTTTTTTGTGGCGGGTATTTGGGGGCTGCGGGGCGCAAAAT‑AGGATTTAACCCCTTTCTTGTTGGGCGttt                                                                  >  4:222184/1‑137 (MQ=255)
                                                                    ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                 <  4:335034/137‑1 (MQ=255)
                                                                          cGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAg                                                           <  6:248958/137‑1 (MQ=255)
                                                                           gggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTGCTGGAATTTTTGCGGGGGGGGGGGAAAAAT‑CGGATTTTTCCCCGTTGTTGTTGGGGGGTTGCCaaata                                                          >  7:251584/1‑135 (MQ=255)
                                                                           gggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGCCGGGAATTTGGGGGCGGGGGGTGGAAAAAT‑AGGATTTAACCCCGTTCGTGTTGGGGGGTTGCCaaata                                                          >  8:315381/1‑135 (MQ=255)
                                                                             gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGGTGGGATTTTGGCGCTGCGGGTGGATAAAT‑CGGTTTTAAACCCGTGGTTGTTGGGGGTTTTCCAAAtata                                                        >  8:186738/1‑137 (MQ=255)
                                                                             gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCGCGGGCGTGGTTTTTTTTGGCGGGAATTTGGGCGCGGCGGGGCGCAAAAA‑CCGATTTAAACCCATTGTTGTAGGGCGGTTCCCAAAGATa                                                        >  8:165662/1‑137 (MQ=255)
                                                                                                 ccaccaAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGCTGGGATTTTGGGGCGGCGGGTGGAAAAAT‑CGGATTTTAACCCGTTGTTGTAGGGGGTTTGCCAAAGATACTGCCTGCGCCTGTTTTACg                                    >  6:56231/1‑137 (MQ=255)
                                                                                                             aagaagaagACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttt                        <  8:312071/137‑1 (MQ=255)
                                                                                                               taagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                      <  8:20350/136‑1 (MQ=255)
                                                                                                               caagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                      <  1:175427/136‑1 (MQ=255)
                                                                                                                 agaagaCGCCCTCGGGCGTGTTTTTTTGTGGCGGGGATTTGGGGGCGGCGGGTCGAAAAAT‑CGGTTTTTACCCCATTGTTGTAGGGGGTTTGCCAAAGATACTTCCTGCGCCTGTTTTCCGTTGAGGTCAttttttt                    >  6:328192/1‑136 (MQ=255)
                                                                                                                     gaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAggg                <  7:104930/137‑1 (MQ=255)
                                                                                                                     gaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAggg                <  5:57272/137‑1 (MQ=255)
                                                                                                                             cGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtc        <  7:122500/137‑1 (MQ=255)
                                                                                                                               ggCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtctc      <  4:201722/137‑1 (MQ=255)
                                                                                                                                   tCTTTTTTTGTGCCTGGAATTAGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTg  <  4:149022/137‑1 (MQ=255)
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GCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAT‑CGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTG  >  ACB143/5383626‑5383893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑