Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1436,043,1960CA25.6% 116.3 / 17.7 78A37S (GCC→TCC) puuD_3Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD
Reads supporting (aligned to +/- strand):  ref base C (29/29);  new base A (0/20);  total (29/49)
Fisher's exact test for biased strand distribution p-value = 2.20e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTGGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCA  >  ACB143/6043063‑6043329
                                                                                                                                     |                                                                                                                                     
cTGGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCaaa                                                                                                                                    >  8:319046/1‑137 (MQ=255)
    gggCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCACGCCGGCAAAgccg                                                                                                                                >  8:25083/1‑137 (MQ=255)
    gggCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAgccg                                                                                                                                >  8:226806/1‑137 (MQ=255)
     ggCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAgccgg                                                                                                                               >  7:90879/1‑137 (MQ=255)
      gcgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAgccggc                                                                                                                              >  6:354835/1‑137 (MQ=255)
      gcgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAgacggc                                                                                                                              >  1:318692/1‑137 (MQ=255)
         ccATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGgccgc                                                                                                                           >  7:107447/1‑137 (MQ=255)
                       cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGt                                                                                                             >  4:21636/1‑137 (MQ=255)
                       cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGt                                                                                                             >  6:24430/1‑137 (MQ=255)
                         gCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCg                                                                                                           >  3:89156/1‑137 (MQ=255)
                            cGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCAGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCg                                                                                                        >  3:110725/1‑137 (MQ=255)
                                   tGACGGCGAGCCGTTAAAAACAATGCCGTGCTGGCGAGCCAGCAGCGGTTGCGTGTCGCGGCCGCCATCACGGGCCGGAAAGATCTGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCgtgt                                                                                                 <  1:266197/137‑1 (MQ=255)
                                            gCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGt                                                                                        >  6:129637/1‑137 (MQ=255)
                                                      aCAATGCCGTGCAGGCGAGCCAGCAGCGCTGGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCCGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTAc                                                                              <  5:56041/137‑1 (MQ=255)
                                                        aaTGCCGTGCAGGCGAGCCAGCAGCGCTTGCGGGTCGCTGCGGCCATCAGGGGCCGGAAAGATCAGGGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACct                                                                            <  4:93704/137‑1 (MQ=255)
                                                         aTGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACctg                                                                           >  7:315059/1‑137 (MQ=255)
                                                               tGCAGGCGTGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCACGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg                                                                     <  6:290412/137‑1 (MQ=255)
                                                               tGCAGGCGAGCCAGCAGCGCTTGCGTGGCGCTGCCGCCATCACGGGCCGGCAAGATCCGGGGCAACCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACAGTGTGCGAGGAGTTCTTCCCTACcttcttatt                                                                     <  5:72066/137‑5 (MQ=255)
                                                               ggCAGGCGCGCCAGCCGCGGTGGGGTGGCGCTGCCGCCATCCCGGGCGGGCAAGATCAGGGGCAAGCCGGAAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg                                                                     <  2:161359/136‑1 (MQ=255)
                                                               ggCAGGCGAGCCCGCCGCGGTTGCGGGGCGCGGCCGCCACCCCGGGCCGGAAAGAGCCGGGGCACGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg                                                                     <  3:21636/136‑1 (MQ=255)
                                                               ggCAGGCGAGCCAGCAGCGCTTGGGTGTCGCTGCCGCCATCAGGGGCGGGCAAGATCAGTGGCAAGCGGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg                                                                     <  7:180044/136‑1 (MQ=255)
                                                               ggCAGGCGAGCCAGCAGCGCTGGCTTGGCGCTGCCGCCATCACGGGCCGCCAAGATCAGTGGAAAGCCGGAAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg                                                                     <  1:183926/136‑1 (MQ=255)
                                                               gcccggCGAGCCAGCCGCGCTGGCGTGGCGCCGCCCCCAACCCGGGCCGCCAAGCGCAGGGCCACGCCGCAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg                                                                     <  5:231864/133‑1 (MQ=255)
                                                                gccggCGACCCACCAGCGCTTGCGGGGCGCGGCCGCCATCCCGGGCCGGAAATAGCTGTGGCAACCCGGCAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACgg                                                                    <  1:30649/134‑1 (MQ=255)
                                                                  aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCa                                                                  >  3:93704/1‑137 (MQ=255)
                                                                  aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCa                                                                  >  1:161359/1‑137 (MQ=255)
                                                                  aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCa                                                                  >  1:256909/1‑137 (MQ=255)
                                                                  aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCa                                                                  >  2:189303/1‑137 (MQ=255)
                                                                  aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCa                                                                  >  7:186519/1‑137 (MQ=255)
                                                                  aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCAGACCGTGTGCGACGAGTTCTTCCCTACATGCAGAAGGCa                                                                  >  5:151854/1‑137 (MQ=255)
                                                                     cGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGgc                                                               >  4:339260/1‑137 (MQ=255)
                                                                         ccAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTg                                                           >  6:87931/1‑137 (MQ=255)
                                                                          ctgcaggGCTTGCGTGTCGCTGCCGCCTTAAGGGGCCGGCAAGATCAGTGGCAAGCGGGCAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa                                                          <  8:203080/135‑1 (MQ=255)
                                                                          ctgcagcGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa                                                          <  6:346690/135‑1 (MQ=255)
                                                                          cagcagCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa                                                          <  4:89156/137‑1 (MQ=255)
                                                                            gcagcGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGaca                                                        >  7:152397/1‑137 (MQ=255)
                                                                                     gCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCaaa                                               >  3:249252/1‑137 (MQ=255)
                                                                                       gtgtCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAgg                                             <  2:256909/137‑1 (MQ=255)
                                                                                       gtgtCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATAAAAgg                                             >  3:214704/1‑137 (MQ=255)
                                                                                               gccgcaATCACGGGCCGGCAATATCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAt                                     <  4:249252/137‑1 (MQ=255)
                                                                                               gccgcaATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAt                                     <  2:183521/137‑1 (MQ=255)
                                                                                                     ataccGGGCCGGCAAGATCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  7:13840/133‑1 (MQ=255)
                                                                                                     acacaaggCCGCCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  5:5157/131‑1 (MQ=255)
                                                                                                     aTCCCGGGGCGGCAAGATCAGTGCCACGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  7:120071/137‑1 (MQ=255)
                                                                                                     aTCCCGGGCCGGAAAGATCAGTGGAACGCCGGAAAAGCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  3:242018/137‑1 (MQ=255)
                                                                                                     aTCACGGGCGGGCAATACATGTGGCAAGCGGGCAAAGCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  2:129506/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCTGTGGCATGCCGGATAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGTGACCGCATTTgcgc                               <  6:151854/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  8:99360/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  7:193256/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  6:150758/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  6:147848/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  5:87931/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  5:354835/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  6:150885/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  5:129637/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  2:10007/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGATATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  5:128597/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  7:194132/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCAGTGCCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  1:216242/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCAGGGGCACGCCGGCAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  4:43884/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGCAAGATCAGGGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  2:852/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGAAAGATCTGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  8:357804/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGAAAGATCAGTGGCAAGCCGGCAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  3:156165/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGAAAGAGCTGGGCCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  3:344288/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGAAAGAGCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  2:97884/137‑1 (MQ=255)
                                                                                                     aTCACGGGCCGGAAAGAGCAGTGCCAACCCGCAAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGTGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc                               <  1:267275/137‑1 (MQ=255)
                                                                                                        aCGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTGTTCCCTACCTGCTGACGGCAGGCGCTGACCACGATCAAAGGGACCGCATTTGGGCTCa                            >  7:336568/1‑137 (MQ=255)
                                                                                                               ggCAAGATCAGTGGCACGCCGGCAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt                     <  8:107447/137‑1 (MQ=255)
                                                                                                               ggCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt                     <  8:186519/137‑1 (MQ=255)
                                                                                                               ggCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt                     <  8:315059/137‑1 (MQ=255)
                                                                                                                 cAAGAGCAGGGGCAAGCCGGCAACGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTac                   <  2:92648/137‑1 (MQ=255)
                                                                                                                  aaGTTCATTGCCATGCGGGAAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTaca                  <  1:120281/137‑1 (MQ=255)
                                                                                                                        cgTGGCACCCCGGCAACCCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgtgt            <  8:218953/136‑1 (MQ=255)
                                                                                                                         gTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGtgtg           >  2:39062/1‑137 (MQ=255)
                                                                                                                             cAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg       >  1:249479/1‑137 (MQ=255)
                                                                                                                             cAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg       <  8:90879/137‑1 (MQ=255)
                                                                                                                                  cGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCa  >  7:218953/1‑137 (MQ=255)
                                                                                                                                  cGGCAAAGCCGCCCGCCTCCACATACTTGTCGCCCACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACAACGATCAAATGGACCGCAATTTCGATCATGGGCTTACAACGAGTGAAGGTACCa  >  6:96950/1‑137 (MQ=255)
                                                                                                                                     |                                                                                                                                     
CTGGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCA  >  ACB143/6043063‑6043329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: