Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 4,759,510 G→A intergenic (+125/‑15) fpvA_3 → / → ligM_KToptimized Ferripyoverdine receptor/sequence generated by OPTIMIZER for KT2440

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,759,5100GA97.5% 134.1 / NA 40intergenic (+125/‑15)fpvA_3/ligM_KToptimizedFerripyoverdine receptor/sequence generated by OPTIMIZER for KT2440
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (16/23);  minor base T (0/1);  total (16/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAA  >  ACB143/4759377‑4759643
                                                                                                                                     |                                                                                                                                     
aGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGAttt                                                                                                                                    <  6:308897/137‑1 (MQ=255)
aGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGAttt                                                                                                                                    <  3:321291/137‑1 (MQ=255)
          cGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGAttt                                                                                                                                    <  4:313393/127‑1 (MQ=255)
          cGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGAttt                                                                                                                                    >  3:313393/1‑127 (MQ=255)
          cGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGtata                                                                                                                          >  7:88418/1‑137 (MQ=255)
          cGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGtata                                                                                                                          >  6:335749/1‑137 (MQ=255)
                aCGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAg                                                                                                                    <  4:238901/137‑1 (MQ=255)
                      aaCTCAAAAAACCGCACACATGTGCGGTTTTTTGATCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGcccc                                                                                                              <  4:36410/137‑1 (MQ=25)
                      aaCGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGcccc                                                                                                              <  7:70041/137‑1 (MQ=255)
                      aaCGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGAGGATAAAAATTTCACACCAACACATAGGATTTAGTAGGTATACATGAGCGcccc                                                                                                              <  3:227128/137‑1 (MQ=255)
                         gCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGAc                                                                                                           >  2:5786/1‑137 (MQ=255)
                                 ccGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTgg                                                                                                   <  5:257699/137‑1 (MQ=255)
                                            tGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTg                                                                                        >  5:262876/1‑137 (MQ=255)
                                                          ttGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCaa                                                                          <  4:218271/136‑1 (MQ=255)
                                                               taCAATTAATCATCGGCTCGTATAATGTTTGGAATTGTGAGCGGATAACTATTTCACACCAACACATAGGATTTTGGATGTATACATGAGCGCCCCGACTAACCTGGTGCAGGTGCTGGCGGCCGGCGGCAACACCg                                                                     <  6:154718/136‑1 (MQ=37)
                                                               taCAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCg                                                                     <  8:323183/136‑1 (MQ=255)
                                                                aCAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATCACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTCACCTGGCGCAGGTGCTGGCCGCCGGCGGCAACACAGt                                                                    >  6:314517/1‑137 (MQ=37)
                                                                aCAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGt                                                                    >  1:233988/1‑137 (MQ=255)
                                                                aCAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGt                                                                    >  6:320129/1‑137 (MQ=255)
                                                                aCAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGt                                                                    >  5:222626/1‑137 (MQ=255)
                                                                         catcGGCTCGTATAATGTGTGGAATTGTGAGCGTATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGtt                                                           <  1:130608/137‑1 (MQ=255)
                                                                          atcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGTTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCTGGTGGTGGCCGCCGGCGGCAACACCGTTGAAATGTTg                                                          <  1:25695/137‑1 (MQ=255)
                                                                          atcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAatgatg                                                          >  6:44663/1‑137 (MQ=255)
                                                                          atcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTg                                                          <  4:176382/137‑1 (MQ=255)
                                                                               cTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTaa                                                     <  5:335749/137‑1 (MQ=255)
                                                                                tCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGTAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAAc                                                    <  3:99879/137‑1 (MQ=255)
                                                                                        tgtgtgGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGAt                                            <  6:262876/137‑1 (MQ=255)
                                                                                           gtgGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTgg                                         <  2:87569/137‑1 (MQ=255)
                                                                                            tgcatttGTGAGCGGATAACAATTTCACACAAACACATAGTATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgc                                        <  3:129151/132‑1 (MQ=255)
                                                                                               aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGcc                                     >  2:88535/1‑137 (MQ=255)
                                                                                               aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGcc                                     >  2:253850/1‑137 (MQ=255)
                                                                                               aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGcc                                     >  3:134804/1‑137 (MQ=255)
                                                                                               aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGcc                                     >  7:126558/1‑137 (MQ=255)
                                                                                               aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGcc                                     >  8:271661/1‑137 (MQ=255)
                                                                                               aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACATGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGcc                                     >  4:211434/1‑137 (MQ=255)
                                                                                                      aGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCt                              <  3:200724/137‑1 (MQ=255)
                                                                                                             aaCAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgt                       <  8:22957/137‑1 (MQ=255)
                                                                                                                             cacaTAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTtc       <  3:17562/137‑1 (MQ=255)
                                                                                                                                  aGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGCGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa  <  1:5786/137‑1 (MQ=255)
                                                                                                                                  aGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa  <  1:88535/137‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
AGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAA  >  ACB143/4759377‑4759643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑