Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,009,212 Δ1 bp 100% intergenic (‑156/+78) CJ019_04705 ← / ← spuC_5 hypothetical protein/Putrescine‑‑pyruvate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,009,2120A.76.7% 131.3 / 72.1 60intergenic (‑156/+78)CJ019_04705/spuC_5hypothetical protein/Putrescine‑‑pyruvate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (6/8);  new base . (23/23);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 7.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

CGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCTGAT  >  ACB143/5009080‑5009343
                                                                                                                                    |                                                                                                                                   
cGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACaaa                                                                                                                                   >  6:260642/1‑135 (MQ=255)
cGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACaaa                                                                                                                                   >  2:178546/1‑135 (MQ=255)
      gatgaATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACaaaaaaaaa                                                                                                                             <  1:230538/135‑1 (MQ=255)
      gatgaATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACaaaaaaaaa                                                                                                                             <  3:132299/135‑1 (MQ=255)
                 ccGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTGGTGAACTGATGACAGCGTCTTGGCTTTGATTGTCATAGCACTAC‑AAAAAAAAAAGCCCGCCTaaa                                                                                                                <  8:136079/136‑1 (MQ=255)
                  cGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTa                                                                                                                  >  2:283728/1‑134 (MQ=255)
                       cGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGCGCGGAAAGTACGTGCTTGTGAACTGATGACAGCGTCTTGGCGTTGATTGTGATCGACCTAC‑AAATAAAAAAGCACCCCTAAAGGAggg                                                                                                          >  4:202574/1‑136 (MQ=255)
                       cGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGCACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGACTAAGGGCggg                                                                                                          >  8:271429/1‑136 (MQ=255)
                       cGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCggg                                                                                                          >  7:100592/1‑136 (MQ=255)
                       cGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCggg                                                                                                          >  2:212268/1‑136 (MQ=255)
                       cGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCggg                                                                                                          >  6:287657/1‑136 (MQ=255)
                            tcCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCtt                                                                                                       >  2:25128/1‑135 (MQ=255)
                            tcCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGGTTa                                                                                                      >  3:149182/1‑135 (MQ=255)
                             ccGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTaa                                                                                                     >  3:102162/1‑135 (MQ=255)
                              cGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTTAACTGATGACAGCGTCTTGGCTTTTATTGTTATAGCACTAC‑‑AAAAAAAAAGCCCGCCTAAAGGCGGGCTTaaa                                                                                                    <  4:10608/134‑1 (MQ=255)
                               ggCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAAc                                                                                                  <  1:84188/136‑1 (MQ=255)
                               ggCGCGGGCTTTTACTTTCCGGGCCATGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATACCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAAc                                                                                                  <  8:200013/136‑1 (MQ=255)
                                gcgcGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAAc                                                                                                  <  6:124271/136‑1 (MQ=255)
                                gcgcGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGACTTAAAAc                                                                                                  <  1:283728/136‑1 (MQ=255)
                                    gggCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAAc                                                                                                  <  8:181748/131‑1 (MQ=255)
                                    gggCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAAc                                                                                                  >  7:181748/1‑131 (MQ=255)
                                    gggCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAaga                                                                                             <  2:108994/136‑1 (MQ=255)
                                                tCCGGGCCTTGGACATTATCTGACCCGAAAGTACGTACTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTTATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAg                                                                                  <  4:34965/135‑1 (MQ=255)
                                                    ggCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGc                                                                             <  4:193539/136‑1 (MQ=255)
                                                     gCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGCCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTggaa                                                                            >  3:271175/1‑134 (MQ=255)
                                                          ggACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGcatca                                                                         <  6:122539/135‑1 (MQ=255)
                                                          ggACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGcatcat                                                                        <  3:24873/135‑1 (MQ=255)
                                                           gACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCAtt                                                                       <  8:111250/135‑1 (MQ=255)
                                                                  tCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑‑AAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGt                                                               <  1:55386/135‑1 (MQ=255)
                                                                   cTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGt                                                               >  5:248139/1‑136 (MQ=255)
                                                                   cTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGtt                                                              >  7:164604/1‑136 (MQ=255)
                                                                   cTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGtt                                                              >  5:71412/1‑136 (MQ=255)
                                                                   cTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTAAGCTGGCATCATTGCCAACGtt                                                              >  1:274119/1‑136 (MQ=255)
                                                                   cTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑‑AAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGtt                                                              >  8:177527/1‑135 (MQ=255)
                                                                      aCCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGt                                                               <  8:34396/133‑1 (MQ=255)
                                                                        ccGAAAGGACGCCCTTGGGAACTGCTGACACCGTCTTGGCGTTGATTGTGAAAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATgg                                                         <  7:110184/136‑1 (MQ=255)
                                                                             aGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCt                                                       >  8:83185/1‑134 (MQ=255)
                                                                             aGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCt                                                       >  4:59477/1‑133 (MQ=255)
                                                                             aGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCt                                                       >  5:225915/1‑133 (MQ=255)
                                                                             aGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCt                                                       >  2:1907/1‑133 (MQ=255)
                                                                             aGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGATTAAAAAGATTATGGTAATTCAGCTGGCATCATTGCCAACGGTGATGGCTCAg                                                    >  4:224665/1‑136 (MQ=255)
                                                                                 cGTCCTTGTTAACTGATTACACCGTCTTGGCTTTTATTGTTATAGCA‑‑ATAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCtt                                               <  5:182299/136‑1 (MQ=255)
                                                                                  gTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCtt                                               <  1:3397/136‑1 (MQ=255)
                                                                                  gTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCtt                                               <  8:196915/136‑1 (MQ=255)
                                                                                   tCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCCGACCtt                                               >  2:107092/1‑135 (MQ=255)
                                                                                            aCTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑‑AAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCtcatca                                     <  3:172956/135‑1 (MQ=255)
                                                                                            aCTGATGACAGAGTATTGGCTTTTTTTTTGATAGCAATA‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACTAAGAGGGTAATTCAGCTGGCATAATTTAAAACGTTGATGGCTCAGACCTTCAGCtcatca                                     <  6:225915/136‑1 (MQ=255)
                                                                                                atgaCAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAgg                                  <  1:192838/135‑1 (MQ=255)
                                                                                                     aGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAGGCCAGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTc                            <  8:77496/136‑1 (MQ=255)
                                                                                                        gTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAg                          >  8:82329/1‑136 (MQ=255)
                                                                                                        gTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAg                          >  5:147938/1‑135 (MQ=255)
                                                                                                        gTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAg                          >  5:244571/1‑135 (MQ=255)
                                                                                                             ggCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGc                    >  5:109858/1‑136 (MQ=255)
                                                                                                              gCTTTTTTTGTGATTGCACTA‑AACAAAAACAACCCCGCCTAAAGGCGGTCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGc                    <  3:159493/135‑1 (MQ=255)
                                                                                                              gCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGc                    <  7:29605/135‑1 (MQ=255)
                                                                                                              gCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGc                    <  4:136327/135‑1 (MQ=255)
                                                                                                              gCTTTCATTGTCATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGc                    <  8:86242/135‑1 (MQ=255)
                                                                                                                   gATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCgt               <  8:164604/135‑1 (MQ=255)
                                                                                                                   gATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCgt               <  3:240078/135‑1 (MQ=255)
                                                                                                                    aTTTTGATAGCACTACAAAAAAAATAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCgt               <  1:97895/135‑1 (MQ=255)
                                                                                                                      ttttaTAGCACTAC‑AAAAATAACAGCACGCCTAAAGGATGGCTTAAAACGAATAGGGTAATTCAGATGGCATCATTGCCAACGTTGATGGCTCAGACCTTCATCTCATCAAGGTCGCTCATCACATCTTCGtgtg            <  1:264492/131‑1 (MQ=255)
                                                                                                                       gtgATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCg          >  8:106891/1‑136 (MQ=255)
                                                                                                                           tAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGc      >  7:11197/1‑136 (MQ=255)
                                                                                                                                 tACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGAGATGCtgat  >  5:276394/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                                   
CGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCTGAT  >  ACB143/5009080‑5009343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑