Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	1,272,812	A→T	6.8%	R79R (CGT→CGA)	CJ019_01194 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	1,272,812	0	A	T	6.8%	222.9 / 4.9	68	R79R (CGT→CGA)	CJ019_01194	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (42/21);  new base T (2/3);  total (44/24)
Fisher's exact test for biased strand distribution p-value = 3.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

AGAGCCTTTGACGGGCGCAATGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACATTTGTTAGAGC  >  ACB143/1272692‑1272945                                                                                                                         |                                                                                                                                      agagCCTTTGACGGGCGCAATGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGAt                                                                                                                         <  2:59896/135‑1 (MQ=255)  gagCCTTTGACGGGCTCAATGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTg                                                                                                                       <  8:23494/136‑1 (MQ=255)  gagCCTTTGACGGGCGCAATGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTg                                                                                                                       <  5:87922/136‑1 (MQ=255)  gagCCTTTGACGGGCGCAATGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTg                                                                                                                       >  6:58694/1‑136 (MQ=255)  gagCCTTTGACGGGCGCAATGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTg                                                                                                                       >  6:6688/1‑136 (MQ=255)  gagCCTTTGACGGGCGCAATGTGGGACAACTCGGATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTg                                                                                                                       <  1:280249/136‑1 (MQ=255)    gCCTTTGACGGGCGCAATGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTc                                                                                                                     >  4:144773/1‑136 (MQ=255)    gCCTTTGACGGGCGCAATGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTc                                                                                                                     >  3:206441/1‑136 (MQ=255)    gCCTTTGACGGGCGCAATGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGATCGCACAGAGCCTCGAGCGTATGGCCACGTAGAATTTCTATTGTc                                                                                                                     >  4:27506/1‑136 (MQ=255)     ccTTTGACGGGCGCAATGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCt                                                                                                                    <  1:211233/136‑1 (MQ=255)             gggCGCAATGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTGTGGCCACGTAGAATTTCGATTGTCTGAGCACgg                                                                                                            <  6:5456/136‑1 (MQ=255)                  cAATGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAg                                                                                                          >  2:137929/1‑133 (MQ=255)                  cAATGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAg                                                                                                          <  1:137929/133‑1 (MQ=255)                    aTGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACt                                                                                                     <  5:84102/136‑1 (MQ=255)                            cAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCg                                                                                             <  5:86123/136‑1 (MQ=255)                                tCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATg                                                                                          >  8:248535/1‑135 (MQ=255)                                  gTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATgtag                                                                                       >  1:209064/1‑136 (MQ=255)                                  gTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATgtag                                                                                       >  6:232615/1‑136 (MQ=255)                                  gTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATgtag                                                                                       >  4:148000/1‑136 (MQ=255)                                  gTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATgtag                                                                                       >  8:62996/1‑136 (MQ=255)                                  gTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATgta                                                                                        <  4:45041/135‑1 (MQ=255)                                            gTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCTCGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAg                                                                             >  8:150950/1‑136 (MQ=255)                                            gTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCTCGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAg                                                                             >  8:154540/1‑136 (MQ=255)                                             tATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAg                                                                             >  7:181153/1‑135 (MQ=255)                                             tATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAg                                                                             >  3:48816/1‑135 (MQ=255)                                                    atatCCGTTCCACTTCATGTTGCTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTc                                                                     <  4:47605/136‑1 (MQ=255)                                                      atCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCAAGCGATGTAGTAGAAGAAAGCAGATTTCa                                                                    >  5:243986/1‑135 (MQ=255)                                                       tCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCa                                                                    >  6:117401/1‑134 (MQ=255)                                                              cACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCttt                                                            >  2:142127/1‑135 (MQ=255)                                                                cTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTc                                                          >  3:143454/1‑135 (MQ=255)                                                                   cATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGc                                                      >  6:211078/1‑136 (MQ=255)                                                                    aTGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCTCGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCg                                                     <  7:150950/136‑1 (MQ=255)                                                                    aTGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCTCGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCg                                                     <  7:154540/136‑1 (MQ=255)                                                                    aTGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCg                                                     >  4:98578/1‑136 (MQ=255)                                                                       ttgttCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGt                                                  <  5:232615/136‑1 (MQ=255)                                                                                  gACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCctcttg                                       >  1:252474/1‑136 (MQ=255)                                                                                       cGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCctcttgctctt                                  >  5:77155/1‑136 (MQ=255)                                                                                        gTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCctcttgctcttg                                 >  4:287523/1‑136 (MQ=255)                                                                                          gAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTc                                                          <  8:134943/109‑1 (MQ=255)                                                                                          gAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTc                                                          >  7:134943/1‑109 (MQ=255)                                                                                          gAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCttgtt                               >  2:12875/1‑136 (MQ=255)                                                                                          gAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCttgtt                               >  1:197471/1‑136 (MQ=255)                                                                                          gAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCttgtt                               >  7:2601/1‑136 (MQ=255)                                                                                          gAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCttgtt                               >  1:271778/1‑136 (MQ=255)                                                                                          gAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCttgtt                               >  6:87857/1‑136 (MQ=255)                                                                                          gAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCttgtt                               >  5:210756/1‑136 (MQ=255)                                                                                                 gCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGt                         <  1:55668/135‑1 (MQ=255)                                                                                                  cacaGAGACTCTAGCGTATGGCCTCGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAg                       <  4:238861/136‑1 (MQ=255)                                                                                                         aCTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTcc                <  7:151520/136‑1 (MQ=255)                                                                                                         aCTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTcc                <  3:57026/136‑1 (MQ=255)                                                                                                          ctctAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTcc                <  8:2601/135‑1 (MQ=255)                                                                                                            ctAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCcaca             >  2:76094/1‑136 (MQ=255)                                                                                                              aGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACAtt           >  3:162502/1‑136 (MQ=255)                                                                                                               gCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACAtt           <  2:125495/135‑1 (MQ=255)                                                                                                               gCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACAtt           <  3:279307/135‑1 (MQ=255)                                                                                                               gCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACAtt           <  7:15237/135‑1 (MQ=255)                                                                                                                cGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAATCGTGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGATCCACATTTg         >  3:38064/1‑136 (MQ=255)                                                                                                                cGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGTTAGTGGCTCCACATTTg         >  8:115845/1‑136 (MQ=255)                                                                                                                cGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACATTTg         >  1:192748/1‑136 (MQ=255)                                                                                                                    tGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGATAGCAGATTTCAAAGCCTTTTCAAGCGGTTCAATCATCTTGCTCTTGTTGCGGGTAGTGGCTCGACATTTGTTag     >  4:188610/1‑136 (MQ=255)                                                                                                                    tGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACATTTGTTa      <  3:52249/135‑1 (MQ=255)                                                                                                                       ccACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACATTTGTTagag   >  7:98978/1‑135 (MQ=255)                                                                                                                       ccACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACATTTGTTagag   >  6:23386/1‑135 (MQ=255)                                                                                                                        cACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACATTTGTTAGAGc  >  1:178622/1‑135 (MQ=255)                                                                                                                        cACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACATTTGTTAGAGc  >  1:252168/1‑135 (MQ=255)                                                                                                                        cACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACATTTGTTAGAGc  >  7:134855/1‑135 (MQ=255)                                                                                                                        cACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACATTTGTTAGAGc  >  6:163297/1‑135 (MQ=255)                                                                                                                        cACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACATTTGTTAGAGc  >  4:171215/1‑135 (MQ=255)                                                                                                                         |                                                                                                                                      AGAGCCTTTGACGGGCGCAATGTGGGACAACTCGTATGTGGATGTATCACCATATCCGTTCCACTTCATGTTGTTCTTATAGACTTCGTGAAGCTCGCACAGAGACTCTAGCGTATGGCCACGTAGAATTTCGATTGTCTGAGCACGGAGGCACTCGCCAGCGATGTAGTAGAAGAAAGCAGATTTCAAAGCCTTTTCAAGCGGGTCAATCCTCTTGCTCTTGTTGCGGGTAGTGGCTCCACATTTGTTAGAGC  >  ACB143/1272692‑1272945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑