Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,171,266 G→C 13.8% A149P (GCA→CCA)  CJ019_02060 → IS1182 family transposase ISPpu16

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,171,2660GC13.8% 282.0 / 8.8 122A149P (GCA→CCA) CJ019_02060IS1182 family transposase ISPpu16
Reads supporting (aligned to +/- strand):  ref base G (59/43);  new base C (2/16);  total (62/60)
Fisher's exact test for biased strand distribution p-value = 2.31e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

AAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTG  >  ACB143/2171134‑2171391
                                                                                                                                      |                                                                                                                             
aaaaCCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAg                                                                                                                            <  5:126276/136‑1 (MQ=255)
       tCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACaa                                                                                                                     >  8:238579/1‑136 (MQ=255)
        cGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACaa                                                                                                                     >  2:5723/1‑135 (MQ=255)
          ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATcg                                                                                                                  >  7:18508/1‑136 (MQ=255)
          ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATcg                                                                                                                  >  4:281511/1‑136 (MQ=255)
          ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGAAACCTCAACCTTTTCTCTGAGTAGATCATTGCAATCGACGGCCGCAAGTTAAAACCAGTGAACAATcg                                                                                                                  >  2:109428/1‑136 (MQ=255)
              cTTCCGAAAGCACAACGTAAAACCCATTCGTAGCTGCTGCCTTCAGTTCGTTATCCTCTGCCTAAACCACAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAACGCAGTCAACAAGCGCGa                                                                                                               <  8:274384/135‑1 (MQ=255)
                   ccAAGGACACCGGAAAAGCCATTCGTAGCGTCTGCCGGCTGTTCGTTATCCTCTGCCTTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGAGGGCAGCAAGTTCAAAGCAGTTAACAATCGCGACCGTa                                                                                                          <  6:275806/134‑1 (MQ=255)
                       ggACAACGGTAAAGCAATTCGTAGCGTCTGCCGTCAGTTGGTGATCCTCTGCCGTAACCTAAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTTAACAATCGCGACCGTAACTTc                                                                                                     <  7:213908/136‑1 (MQ=255)
                       ggAAAACGTTAAAGCCATTCGTCGCGGCTGCCGTCAGTTGGTGATCCTCTGCCGTAACCTCACCATTTTCTCTGATTCTATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTCAAAAATCGCGACCGTAACTTc                                                                                                     <  8:154423/136‑1 (MQ=255)
                              ttaaaaGCCATTCTTAGCTTCTGCAGTTTGTGCGTTATCTTAGCCCCAAACCAAAACCTTTTATCTTAGCCTATTATTGCCTTCGACGGCTGAATTTCAAATGCTGTGAACAATCGCGACCGTAACTTCACCCAgg                                                                                              <  2:32731/133‑1 (MQ=255)
                              gtaaaaGCATTTCGTAGCGTCTGCCTTCTGTTCGTTATCCTCTGCCTAAACCTAAACTTTTTCTGTGCGTCTATCATTGCCATCGAGGGAAGCAAGTTCAACGCAGTTAACAAGCGCGACCGTAACTTCACCCAgg                                                                                              <  8:68002/133‑1 (MQ=255)
                              gtaaaaCCCATTCTTCGCGTCTGCCGTCATTTCGTGATCCTCTGCCGAAACCTAAACCTTTTCTCTGAGTCTATCATTCCAATCGACGGCAGAATTTTCAAAGCTGTAAAAAACCGCGACCGTAACTTCACCCAgg                                                                                              <  2:18784/133‑1 (MQ=255)
                                   cgCCATTCGTCGCGTCGCCCGGCTGTTCGTGATCCTCTGCCGAACCCACAACGTTTTCTCTGAGTCGATCTTTCCCCGCCAGGGCAGCAATTACAAAGCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAg                                                                                         <  6:253444/135‑1 (MQ=255)
                                     ccATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATAGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                       >  1:245257/1‑136 (MQ=255)
                                      cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                       >  3:291990/1‑135 (MQ=255)
                                           gTAGTGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                 >  7:134447/1‑136 (MQ=255)
                                           gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGCACAATCGCGACCGTAACTTCACCCCGGCCAAGGTGAAGGc                                                                                 >  2:111223/1‑136 (MQ=255)
                                           gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCCGGCCAAGGTGAAGGc                                                                                 >  2:108628/1‑136 (MQ=255)
                                           gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                 >  7:30053/1‑136 (MQ=255)
                                           gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                 >  3:204473/1‑136 (MQ=255)
                                           gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                 >  8:197299/1‑136 (MQ=255)
                                           gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAAAAATCGCGCCCCTAACTTCCCCCAGGCCAAGGTGAAGGc                                                                                 >  2:67447/1‑136 (MQ=255)
                                            tAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTGCAAAGCAGTGAACAATCGCGACCGTAACTTCCCCCAGGCCAAGGTGAAGGcc                                                                                >  3:21566/1‑136 (MQ=255)
                                              gggTCGGCCTTCAGTTCGTCCACCTCTGCCGAAACCACAACCTTTTCGCTCTGCCCACAATGGCCACCGCCGCCAGCAAGTAAAACGCAGTAAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGccc                                                                               <  4:9552/133‑1 (MQ=255)
                                              gCGTCTGACGTCCTTTCGTGATCGAGTGCCGTACCCTCAACCTTTTCTCTGTGCCTATCATTGCCATCTACGGCAGAAAGTTCAAAGCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGccc                                                                               <  6:137429/135‑1 (MQ=255)
                                                  cTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                          >  6:280020/1‑136 (MQ=255)
                                                   tcccGTCAGTTCGTCAGCATGTCCCTAAACCTAAACTTTTTCTCTGTGTCTAAATTGCCCAGCCCGGGCATCAATTAAAAAGCCGTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgc                                                                         <  2:171779/134‑1 (MQ=255)
                                                      cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTTAAATCGACGGCAGCAAGTTCAAACCAGTGAACAAACGCGACCGTAACTTCACCCACGCCAAGGTTAAGGCCCGCATgaag                                                                       >  1:102292/1‑135 (MQ=255)
                                                      cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCACGTTCAAAGCAGTGAACAATCGCGCCCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgccg                                                                       >  8:67110/1‑133 (MQ=255)
                                                      cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                       >  5:45092/1‑135 (MQ=255)
                                                      cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                       >  5:283348/1‑135 (MQ=255)
                                                      cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                       >  4:168280/1‑135 (MQ=255)
                                                      cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                       >  4:37130/1‑135 (MQ=255)
                                                      cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATAGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTAACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                       >  8:125555/1‑135 (MQ=255)
                                                        tCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcagca                                                                     >  5:271319/1‑135 (MQ=255)
                                                           gTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATc                                                                 >  4:13468/1‑136 (MQ=255)
                                                            tgcGTGAGCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGTCGGCAGCAAGTTCAACGCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg                                                                <  2:191056/134‑1 (MQ=255)
                                                              ggTGAGCCTCTGCCGAACCCTAAACTTTTTTTCTGTGTGTATAATTCCCATCGAGGGCAGAAATTAAAACGAAGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                               <  5:88086/134‑1 (MQ=255)
                                                              cGTGATCCTCTGCCGTAACCTAAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAACCCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                               <  7:26899/135‑1 (MQ=255)
                                                               gTGATCCTCTGCCGTAACCTCAACATTTTCTCTGAGTCGATCATTTCCATCGACGGCAGCAAGTTCAAATCAGTGAACAATCGCGACCGTAACTTCCCCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgag                                                              >  1:66584/1‑135 (MQ=255)
                                                                   tCCGCTGCCTTAACATCAAATTTTTCTGTGAGTGGATCATTCCCATCTACGGCAGAATTTACAACCCTTTTAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcaga                                                          <  4:142153/135‑1 (MQ=255)
                                                                     ctctGCCTTACACACACCCTTTTCTCTGAGTCTATCATTGCCATCGCGGGCAGCAAGATAACACACGCCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagagca                                                       <  6:25316/136‑1 (MQ=255)
                                                                     ctctGCCTTAACCTCAACCTTTTCTCTTAGTCGTTCTTTCCCATCTCCGGCTGCATGTTCAATCCAGTCAACATTCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagagca                                                       <  7:157472/136‑1 (MQ=255)
                                                                      tctGCCGTAACCTCATCATTTTCTGTGTGTCTATCATTGCCAGCGAGGGCAGCAATTTCAACCCAGTCAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAt                                                      <  1:79264/136‑1 (MQ=255)
                                                                       ctGCCTTAACCACAACTTTTAGTCTGAGACAATAATTCCCATCGACGGCAGAATTTTCAAACCAGTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                     <  1:152537/136‑1 (MQ=255)
                                                                       ctGCCGTAACCTCAACCTTTTCTCTGAGTCGATAATTGCCATCGAGGGCAGAAAGTTCAAAGCAGTGAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                     <  2:25/136‑1 (MQ=255)
                                                                         gcctaaaCCACAACGTTTTCTCTGAGTCCATCATTGCCATCGAGGGCAGAAAGTCAAACCCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  4:34387/131‑1 (MQ=255)
                                                                         gCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                     >  8:132514/1‑134 (MQ=255)
                                                                         gCCGTAACCTCAACATTTTCTCTCAGTGTATCATGGACATCTAGGGCAGCATTTTCAAAGCATTAAACATTCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  4:251510/136‑1 (MQ=255)
                                                                         gCCGCACCCTCACCCTTTGCTCTGAGGCTATCATTGCCATCGACGCCAGCAAGTTCAAAGACGTCAACAACCGCGACCGTAACTTCACCCAGGCCGAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  6:43775/136‑1 (MQ=255)
                                                                         gCCGAAACCTCAACATTTTCTCTGAGTCTATCATTGCCATCGACGGCAGCAAGTTCAAAGCTTTCAAAAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  7:279084/136‑1 (MQ=255)
                                                                         cccttAACCTCAACCTTTTCTCTTAGTCGATCATGCCCATCCACGGCAGCAATTCAAACCCAGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  3:66802/132‑1 (MQ=255)
                                                                          ccttacccTCAACCTTTTCTCTGATGCTATCATTGCCATCGCGGGCAGCACGTTCAAACCCGTCAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAg                                                  <  2:29650/130‑1 (MQ=255)
                                                                          ccttAACCTCAACCTTTTCTCTGAGTCGATCATTCCAATCGAGGGCAGCAAGTTAAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  3:31127/132‑1 (MQ=255)
                                                                          ccttAACATCAACCTTTTCTCTGAGTCTATCATTCCAATCGACGGCACAAAGTTCAAAGAATTCAAAAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  3:233041/132‑1 (MQ=255)
                                                                          ccgaacccTCAACCTTTCATCGGAGTGCTTAATTCCCATCTACGGCAGAATTTTAAACGCAGTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  4:254011/129‑1 (MQ=255)
                                                                          ccGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  7:212087/135‑1 (MQ=255)
                                                                          ccGTAACCTCAACCTTTTCTCTGAGTCCATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAg                                                  <  6:209726/136‑1 (MQ=255)
                                                                          ccGTAACCTAAACCTTTTCTCTGAGCCTATAATTGCCATCGACGGCAGAAATTTAAAAGCAGTAAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  5:197819/135‑1 (MQ=255)
                                                                          ccGAAACCTCAACTTTTTCTCTGCGTCTATATTTGCCAGCGAGGGCAGCATTTTCAAACCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  5:233043/135‑1 (MQ=255)
                                                                          ccGAAACCTCAACCTTTTCTCTGCGGCTATCATGGCAATCGACGGAAGCAAGTTCAACGCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  2:185588/135‑1 (MQ=255)
                                                                          ccGAAACCTAAACCTTTTTTCTTTGTCGATCTTTCCCATGCCCGGCAGCAAGTTCAAACCTTTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  4:105626/135‑1 (MQ=255)
                                                                           cGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAgc                                                 >  8:215153/1‑136 (MQ=255)
                                                                                ccTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAc                                            >  4:232624/1‑136 (MQ=255)
                                                                                ccTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGCTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAc                                            >  6:182006/1‑136 (MQ=255)
                                                                                 cTCAACCTTTTCTCTGAGTCGATAATTGCCATCGACGGCAGCAAGTTCAAAGCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc                                           <  2:15689/136‑1 (MQ=255)
                                                                                 cTCAACATTTTCTCTTAGTCGATCTTTGCCAGCGACGGCAGCAAGTTCAAAGCTGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc                                           <  3:227447/136‑1 (MQ=255)
                                                                                 cTCAACATTTTCTCTGATTCGATCTTGCCCATCGACGGCAGAAAGTTCAACGCTGTAAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc                                           <  8:264358/136‑1 (MQ=255)
                                                                                  tCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCCCCACCGAAACTTCACCCCGGCCAAGGAGAAGGCCCGCATGCAGCAAAACGAGCAGAGCATTGAGCGCTACCt                                          >  6:63848/1‑136 (MQ=255)
                                                                                      ctttttATCTGTGCCTATCATTCCCATCTCCGGCACCAAGTCAAACGCTCTAAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACa                                      <  3:160107/134‑1 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   >  8:196106/1‑122 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  7:196106/122‑1 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTCCCTTACAg                                     >  2:103265/1‑136 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                     >  3:118843/1‑136 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                     >  5:188644/1‑136 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                     >  1:191114/1‑136 (MQ=255)
                                                                                              ctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAATGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCTATGGa                              >  2:260847/1‑136 (MQ=255)
                                                                                              ctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGa                              >  6:187775/1‑136 (MQ=255)
                                                                                                    cGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                         >  5:258647/1‑135 (MQ=255)
                                                                                                    cGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                         >  1:243996/1‑135 (MQ=255)
                                                                                                     gATCATTGCCATCGAGGGCAGCAAGTTCAAAGCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                        <  7:29509/135‑1 (MQ=255)
                                                                                                       tctttGCCATCGACGGCAGCAAGTTCAAAGCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                        <  6:49490/130‑1 (MQ=255)
                                                                                                         aTTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                   >  1:175759/1‑136 (MQ=255)
                                                                                                         aTTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                   >  5:96051/1‑136 (MQ=255)
                                                                                                         aTTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                   >  8:177987/1‑136 (MQ=255)
                                                                                                          atGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                  >  1:102720/2‑136 (MQ=255)
                                                                                                          atGCCAGCCACGTCAGCACGTACAAACCAGTCACCAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                  <  4:28992/135‑1 (MQ=255)
                                                                                                           ttcccATCGCCGGCATCAACTTCAAAGAAGTTAACACCCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTAACAGCGATGGATTCGGCAGACCg                  <  3:298166/133‑1 (MQ=255)
                                                                                                           ttcccATCCACGCCAGCAAGTTCAACGCCGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                  <  1:22823/133‑1 (MQ=255)
                                                                                                               cATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTAACCCAGGCCAAGGTTAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc               >  7:284219/1‑135 (MQ=255)
                                                                                                                atcgcgggCAGCAAGTTCAACCCATTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCaa             <  6:271319/130‑1 (MQ=255)
                                                                                                                     cGGCCGCAAGTTCAAAGCCGGCACCAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg        <  6:282085/136‑1 (MQ=255)
                                                                                                                     cGGCATCAAGTTCCACTCTGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg        <  5:199630/136‑1 (MQ=255)
                                                                                                                     cGGCAGCAAGTTCAACGCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg        <  6:113722/136‑1 (MQ=255)
                                                                                                                     cGGCAGAAAGTTCAACGCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg        <  7:79980/136‑1 (MQ=255)
                                                                                                                         agcaAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAgg    >  5:121035/1‑136 (MQ=255)
                                                                                                                         agcaAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAgg    >  4:4662/1‑136 (MQ=255)
                                                                                                                         agcaAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAgg    >  3:80225/1‑136 (MQ=255)
                                                                                                                           aaaGTTCAAAGCAGATAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg  <  2:259126/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                             
AAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTG  >  ACB143/2171134‑2171391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: