Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB143 | 2,171,266 | G→C | 13.8% | A149P (GCA→CCA) | CJ019_02060 → | IS1182 family transposase ISPpu16 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB143 | 2,171,266 | 0 | G | C | 13.8% | 282.0 / 8.8 | 122 | A149P (GCA→CCA) | CJ019_02060 | IS1182 family transposase ISPpu16 |
Reads supporting (aligned to +/- strand): ref base G (59/43); new base C (2/16); total (62/60) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.31e-04 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 122 total aligned reads displayed.
AAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTG > ACB143/2171134‑2171391 | aaaaCCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAg < 5:126276/136‑1 (MQ=255) tCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACaa > 8:238579/1‑136 (MQ=255) cGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACaa > 2:5723/1‑135 (MQ=255) ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATcg > 7:18508/1‑136 (MQ=255) ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATcg > 4:281511/1‑136 (MQ=255) ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGAAACCTCAACCTTTTCTCTGAGTAGATCATTG‑CAATCGACGGCCGCAAGTTAAAA‑CCAGTGAACAATcg > 2:109428/1‑136 (MQ=255) cTTCCGAAAGCACAACGTAAAACCCATTCGTAGCTGCTGCCTTCAGTTCGTTATCCTCTGCCTAAACCACAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAC‑GCAGTCAACAAGCGCGa < 8:274384/135‑1 (MQ=255) ccAAGGACACCGGAAAAGCCATTCGTAGCGTCTGCCGGCTGTTCGTTATCCTCTGCCTTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGAGGGCAGCAAGTTCAAA‑GCAGTTAACAATCGCGACCGTa < 6:275806/134‑1 (MQ=255) ggACAACGGTAAAGCAATTCGTAGCGTCTGCCGTCAGTTGGTGATCCTCTGCCGTAACCTAAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTTAACAATCGCGACCGTAACTTc < 7:213908/136‑1 (MQ=255) ggAAAACGTTAAAGCCATTCGTCGCGGCTGCCGTCAGTTGGTGATCCTCTGCCGTAACCTCACCATTTTCTCTGATTCTATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTCAAAAATCGCGACCGTAACTTc < 8:154423/136‑1 (MQ=255) ttaaaaGCCATTCTTAGCTTCTGCAGTTTGTGCGTTATCTTAGCCCCAAACCAAAACCTTTTATCTTAGCCTATTATTG‑CCTTCGACGGCTG‑AATTTCAAATGCTGTGAACAATCGCGACCGTAACTTCACCCAgg < 2:32731/133‑1 (MQ=255) gtaaaaGCATTTCGTAGCGTCTGCCTTCTGTTCGTTATCCTCTGCCTAAACCTAAACTTTTTCTGTGCGTCTATCATTG‑CCATCGAGGGAAGCAAGTTCAAC‑GCAGTTAACAAGCGCGACCGTAACTTCACCCAgg < 8:68002/133‑1 (MQ=255) gtaaaaCCCATTCTTCGCGTCTGCCGTCATTTCGTGATCCTCTGCCGAAACCTAAACCTTTTCTCTGAGTCTATCATTC‑CAATCGACGGCAGAATTTTCAAA‑GCTGTAAAAAACCGCGACCGTAACTTCACCCAgg < 2:18784/133‑1 (MQ=255) cgCCATTCGTCGCGTCGCCCGGCTGTTCGTGATCCTCTGCCGAACCCACAACGTTTTCTCTGAGTCGATCTTTC‑CCCGCCAGGGCAGCAATTACAAA‑GCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAg < 6:253444/135‑1 (MQ=255) ccATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATAGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt > 1:245257/1‑136 (MQ=255) cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt > 3:291990/1‑135 (MQ=255) gTAGTGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc > 7:134447/1‑136 (MQ=255) gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGCACAATCGCGACCGTAACTTCACCCCGGCCAAGGTGAAGGc > 2:111223/1‑136 (MQ=255) gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCCGGCCAAGGTGAAGGc > 2:108628/1‑136 (MQ=255) gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc > 7:30053/1‑136 (MQ=255) gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc > 3:204473/1‑136 (MQ=255) gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc > 8:197299/1‑136 (MQ=255) gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAAAAATCGCGCCCCTAACTTCCCCCAGGCCAAGGTGAAGGc > 2:67447/1‑136 (MQ=255) tAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTGCAAA‑GCAGTGAACAATCGCGACCGTAACTTCCCCCAGGCCAAGGTGAAGGcc > 3:21566/1‑136 (MQ=255) gggTCGGCCTTCAGTTCGTCCACCTCTGCCGAAACCACAACCTTTTCGCTCTGCCCACAATGG‑CCACCGCCGCCAGCAAGTAAAAC‑GCAGTAAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGccc < 4:9552/133‑1 (MQ=255) gCGTCTGACGTCCTTTCGTGATCGAGTGCCGTACCCTCAACCTTTTCTCTGTGCCTATCATTG‑CCATCTACGGCAGAAAGTTCAAA‑GCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGccc < 6:137429/135‑1 (MQ=255) cTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg > 6:280020/1‑136 (MQ=255) tcccGTCAGTTCGTCAGCATGTCCCTAAACCTAAACTTTTTCTCTGTGTCTA‑AATTGCCCAGCCCGGGCATCAATTAAAAA‑GCCGTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgc < 2:171779/134‑1 (MQ=255) cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTT‑AAATCGACGGCAGCAAGTTCAAA‑CCAGTGAACAAACGCGACCGTAACTTCACCCACGCCAAGGTTAAGGCCCGCATgaag > 1:102292/1‑135 (MQ=255) cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCACGTTCAAA‑GCAGTGAACAATCGCGCCCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgccg > 8:67110/1‑133 (MQ=255) cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag > 5:45092/1‑135 (MQ=255) cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag > 5:283348/1‑135 (MQ=255) cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag > 4:168280/1‑135 (MQ=255) cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag > 4:37130/1‑135 (MQ=255) cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATAGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTAACCCAGGCCAAGGTGAAGGCCCGCATgcag > 8:125555/1‑135 (MQ=255) tCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcagca > 5:271319/1‑135 (MQ=255) gTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATc > 4:13468/1‑136 (MQ=255) tgcGTGAGCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGTCGGCAGCAAGTTCAAC‑GCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg < 2:191056/134‑1 (MQ=255) ggTGAGCCTCTGCCGAACCCTAAACTTTTTTTCTGTGTGTATAATTC‑CCATCGAGGGCAGAAATTAAAAC‑GAAGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga < 5:88086/134‑1 (MQ=255) cGTGATCCTCTGCCGTAACCTAAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAC‑CCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga < 7:26899/135‑1 (MQ=255) gTGATCCTCTGCCGTAACCTCAACATTTTCTCTGAGTCGATCATTT‑CCATCGACGGCAGCAAGTTCAAA‑TCAGTGAACAATCGCGACCGTAACTTCCCCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgag > 1:66584/1‑135 (MQ=255) tCCGCTGCCTTAACATCAAATTTTTCTGTGAGTGGATCATTC‑CCATCTACGGCAGAATTTACAAC‑CCTTTTAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcaga < 4:142153/135‑1 (MQ=255) ctctGCCTTACACACACCCTTTTCTCTGAGTCTATCATTG‑CCATCGCGGGCAGCAAGATAACA‑CACGCCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagagca < 6:25316/136‑1 (MQ=255) ctctGCCTTAACCTCAACCTTTTCTCTTAGTCGTTCTTTC‑CCATCTCCGGCTGCATGTTCAAT‑CCAGTCAACATTCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagagca < 7:157472/136‑1 (MQ=255) tctGCCGTAACCTCATCATTTTCTGTGTGTCTATCATTG‑CCAGCGAGGGCAGCAATTTCAAC‑CCAGTCAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAt < 1:79264/136‑1 (MQ=255) ctGCCTTAACCACAACTTTTAGTCTGAGACAATAATTC‑CCATCGACGGCAGAATTTTCAAA‑CCAGTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt < 1:152537/136‑1 (MQ=255) ctGCCGTAACCTCAACCTTTTCTCTGAGTCGATAATTG‑CCATCGAGGGCAGAAAGTTCAAA‑GCAGTGAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt < 2:25/136‑1 (MQ=255) gcctaaaCCACAACGTTTTCTCTGAGTCCATCATTG‑CCATCGAGGGCAGAAAGTCAAAC‑CCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 4:34387/131‑1 (MQ=255) gCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt > 8:132514/1‑134 (MQ=255) gCCGTAACCTCAACATTTTCTCTCAGTGTATCATGG‑ACATCTAGGGCAGCATTTTCAAA‑GCATTAAACATTCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 4:251510/136‑1 (MQ=255) gCCGCACCCTCACCCTTTGCTCTGAGGCTATCATTG‑CCATCGACGCCAGCAAGTTCAAA‑GACGTCAACAACCGCGACCGTAACTTCACCCAGGCCGAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 6:43775/136‑1 (MQ=255) gCCGAAACCTCAACATTTTCTCTGAGTCTATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCTTTCAAAAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 7:279084/136‑1 (MQ=255) cccttAACCTCAACCTTTTCTCTTAGTCGATCATGC‑CCATCCACGGCAGCAA‑TTCAAACCCAGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 3:66802/132‑1 (MQ=255) ccttacccTCAACCTTTTCTCTGATGCTATCATTG‑CCATCGCGGGCAGCACGTTCAAA‑CCCGTCAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAg < 2:29650/130‑1 (MQ=255) ccttAACCTCAACCTTTTCTCTGAGTCGATCATTC‑CAATCGAGGGCAGCAAGTTAAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 3:31127/132‑1 (MQ=255) ccttAACATCAACCTTTTCTCTGAGTCTATCATTC‑CAATCGACGGCACAAAGTTCAAA‑GAATTCAAAAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 3:233041/132‑1 (MQ=255) ccgaacccTCAACCTTTCATCGGAGTGCTTAATTC‑CCATCTACGGCAGAATTTTAAAC‑GCAGTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 4:254011/129‑1 (MQ=255) ccGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 7:212087/135‑1 (MQ=255) ccGTAACCTCAACCTTTTCTCTGAGTCCATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAg < 6:209726/136‑1 (MQ=255) ccGTAACCTAAACCTTTTCTCTGAGCCTATAATTG‑CCATCGACGGCAGAAATTTAAAA‑GCAGTAAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 5:197819/135‑1 (MQ=255) ccGAAACCTCAACTTTTTCTCTGCGTCTATATTTG‑CCAGCGAGGGCAGCATTTTCAAA‑CCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 5:233043/135‑1 (MQ=255) ccGAAACCTCAACCTTTTCTCTGCGGCTATCATGG‑CAATCGACGGAAGCAAGTTCAAC‑GCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 2:185588/135‑1 (MQ=255) ccGAAACCTAAACCTTTTTTCTTTGTCGATCTTTC‑CCATGCCCGGCAGCAAGTTCAAA‑CCTTTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 4:105626/135‑1 (MQ=255) cGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAgc > 8:215153/1‑136 (MQ=255) ccTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAc > 4:232624/1‑136 (MQ=255) ccTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGCTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAc > 6:182006/1‑136 (MQ=255) cTCAACCTTTTCTCTGAGTCGATAATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc < 2:15689/136‑1 (MQ=255) cTCAACATTTTCTCTTAGTCGATCTTTG‑CCAGCGACGGCAGCAAGTTCAAA‑GCTGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc < 3:227447/136‑1 (MQ=255) cTCAACATTTTCTCTGATTCGATCTTGC‑CCATCGACGGCAGAAAGTTCAAC‑GCTGTAAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc < 8:264358/136‑1 (MQ=255) tCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCCCCACCGAAACTTCACCCCGGCCAAGGAGAAGGCCCGCATGCAGCAAAACGAGCAGAGCATTGAGCGCTACCt > 6:63848/1‑136 (MQ=255) ctttttATCTGTGCCTATCATTC‑CCATCTCCGGCACCAAGTCAAAC‑GCTCTAAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACa < 3:160107/134‑1 (MQ=255) cTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa > 8:196106/1‑122 (MQ=255) cTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 7:196106/122‑1 (MQ=255) cTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTCCCTTACAg > 2:103265/1‑136 (MQ=255) cTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg > 3:118843/1‑136 (MQ=255) cTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg > 5:188644/1‑136 (MQ=255) cTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg > 1:191114/1‑136 (MQ=255) ctGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAATGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCTATGGa > 2:260847/1‑136 (MQ=255) ctGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGa > 6:187775/1‑136 (MQ=255) cGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg > 5:258647/1‑135 (MQ=255) cGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg > 1:243996/1‑135 (MQ=255) gATCATTG‑CCATCGAGGGCAGCAAGTTCAAA‑GCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc < 7:29509/135‑1 (MQ=255) tctttG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc < 6:49490/130‑1 (MQ=255) aTTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc > 1:175759/1‑136 (MQ=255) aTTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc > 5:96051/1‑136 (MQ=255) aTTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc > 8:177987/1‑136 (MQ=255) atG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg > 1:102720/2‑136 (MQ=255) atG‑CCAGCCACGTCAGCACGTACAAA‑CCAGTCACCAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg < 4:28992/135‑1 (MQ=255) ttcccATCGCCGGCATCAACTTCAAA‑GAAGTTAACACCCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTAACAGCGATGGATTCGGCAGACCg < 3:298166/133‑1 (MQ=255) ttcccATCCACGCCAGCAAGTTCAAC‑GCCGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg < 1:22823/133‑1 (MQ=255) cATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTAACCCAGGCCAAGGTTAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc > 7:284219/1‑135 (MQ=255) atcgcgggCAGCAAGTTCAAC‑CCATTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCaa < 6:271319/130‑1 (MQ=255) cGGCCGCAAGTTCAAA‑GCCGGCACCAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg < 6:282085/136‑1 (MQ=255) cGGCATCAAGTTCCAC‑TCTGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg < 5:199630/136‑1 (MQ=255) cGGCAGCAAGTTCAAC‑GCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg < 6:113722/136‑1 (MQ=255) cGGCAGAAAGTTCAAC‑GCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg < 7:79980/136‑1 (MQ=255) agcaAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAgg > 5:121035/1‑136 (MQ=255) agcaAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAgg > 4:4662/1‑136 (MQ=255) agcaAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAgg > 3:80225/1‑136 (MQ=255) aaaGTTCAAA‑GCAGATAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg < 2:259126/135‑1 (MQ=255) | AAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTG > ACB143/2171134‑2171391 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |