Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,176,936 C→A 12.9% intergenic (+110/‑64) CJ019_02067 → / → CJ019_02068 hypothetical protein/Tn3 family transposase ISYps3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,176,9360CA12.9% 449.0 / 15.5 194intergenic (+110/‑64)CJ019_02067/CJ019_02068hypothetical protein/Tn3 family transposase ISYps3
Reads supporting (aligned to +/- strand):  ref base C (92/73);  new base A (2/26);  total (95/99)
Fisher's exact test for biased strand distribution p-value = 7.13e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

ACTACACCTACAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAGTTACAACGTCAGCTATTGACGC  >  ACB143/2176804‑2177069
                                                                                                                                    |                                                                                                                                     
aCTACACCTACAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCAt                                                                                                                                     >  2:176104/1‑135 (MQ=255)
   acacCTACAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGt                                                                                                                                 >  1:105238/1‑136 (MQ=255)
   acacCTACAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGt                                                                                                                                 >  2:253151/1‑136 (MQ=255)
   acacCTACAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGt                                                                                                                                 >  5:121207/1‑136 (MQ=255)
     acCTACAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGttt                                                                                                                               >  4:183930/1‑136 (MQ=255)
     acCTACAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGttt                                                                                                                               >  2:103839/1‑136 (MQ=255)
     acCTACAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGttt                                                                                                                               >  8:173601/1‑136 (MQ=255)
        tACAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTc                                                                                                                            >  4:90141/1‑136 (MQ=255)
        tACAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTc                                                                                                                            >  2:97407/1‑136 (MQ=255)
        tACAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTc                                                                                                                            >  2:159313/1‑136 (MQ=255)
        tACAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATATTGCTGGGTTCGTGTTGACGCATAAGTTTTTc                                                                                                                            >  4:237234/1‑136 (MQ=255)
        tACAAATTAAATTGATCAAAATTGGGTAAGCGGTACGTAAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACCGCACTATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCTAATAAGCTTGTc                                                                                                                            >  1:227456/1‑136 (MQ=255)
          cAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTc                                                                                                                            <  6:184314/134‑1 (MQ=255)
          cAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCg                                                                                                                           >  1:214879/1‑135 (MQ=255)
             aTTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCaaa                                                                                                                       >  7:283739/1‑136 (MQ=255)
             aTTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCaaa                                                                                                                       >  2:187159/1‑136 (MQ=255)
                 aaTTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAAtggt                                                                                                                   <  1:1819/136‑1 (MQ=255)
                 aaTTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAAtggt                                                                                                                   <  2:39518/136‑1 (MQ=255)
                 aaTTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAAtggt                                                                                                                   <  4:155151/136‑1 (MQ=255)
                 aaTTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAAtggt                                                                                                                   <  1:97924/136‑1 (MQ=255)
                 aaTTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAAtggt                                                                                                                   <  1:32486/136‑1 (MQ=255)
                    tGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAAtggtgg                                                                                                                 >  7:30718/1‑135 (MQ=255)
                    tGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATggtggt                                                                                                                >  8:5328/1‑136 (MQ=255)
                         aataTTGGGTAAGCGGTACGTCAGGTTGTATGTTTTCAAACTGTCGCATTAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCATATGGTGGTATGCt                                                                                                           <  4:193857/133‑1 (MQ=255)
                         aaaaTTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAATTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTAGCATAAGTTTGTCGCAAATGGTGGTATGCt                                                                                                           <  2:35179/136‑1 (MQ=255)
                           aaTTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCtt                                                                                                          >  7:80003/1‑135 (MQ=255)
                                        gTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAAcgc                                                                                            >  5:199413/1‑136 (MQ=255)
                                        gTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAAcgc                                                                                            >  3:166760/1‑136 (MQ=255)
                                           cGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTGGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGac                                                                                         <  5:287160/136‑1 (MQ=255)
                                             tCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGGTGGGTTCGGGTTGTCGCATATGTTTGTCGCAAATGGTGGTATGCTTAAAATAATAAACGCGacac                                                                                       <  8:263953/136‑1 (MQ=255)
                                             tCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAATAATAAACGCGacac                                                                                       <  4:282894/136‑1 (MQ=255)
                                                  ttttATGTTTAAAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTTATTTTGCTGGGTTGGGGTTGTCGCATATTTTTGTCGCAAATGGTGGTATGCTTAAAATAATAAACGCGACACAAAAg                                                                                  <  1:104333/133‑1 (MQ=255)
                                                  ttGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCTATCTCTCGTGTTTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTTGTTTGCTTAAAATAATAAACGCGACACAAAAg                                                                                  <  3:95458/136‑1 (MQ=255)
                                                  ttGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAg                                                                                  <  7:86136/136‑1 (MQ=255)
                                                  ttGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAg                                                                                  <  1:25726/136‑1 (MQ=255)
                                                  ttGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGAATAAGTTTGTCGAAAATGGTGGTATGCTTAAAATAATAAACGCGACACAAAAg                                                                                  <  1:41146/136‑1 (MQ=255)
                                                  ttGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTGCGGGTTGTCGCATATGTTTGTCGCAATTGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAg                                                                                  <  5:268029/136‑1 (MQ=255)
                                                   tgtatgtaTACAAAATTTCGAATATGTCTTTATTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTGTCAAATGGTGTTATGATAAATAGAAAAAACGCGACACAAAAg                                                                                  <  5:215428/135‑1 (MQ=255)
                                                   tGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAgg                                                                                 >  6:206847/1‑136 (MQ=255)
                                                     tATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGc                                                                                <  5:96271/135‑1 (MQ=255)
                                                      aTGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGca                                                                               >  7:148639/1‑135 (MQ=255)
                                                          ttACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGa                                                                          >  4:32811/1‑136 (MQ=255)
                                                          ttACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGAACAGGa                                                                          >  7:213812/1‑136 (MQ=255)
                                                          ttAAAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGTTTTTGCTGGGTTCGGGTTGTCGAATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAgg                                                                           <  6:199648/135‑1 (MQ=255)
                                                            aaaaaCTGTAGCATAAGTTTTATGTTATGCGACAGCCCGTTCTCTCGTGATTTTGGTGGGTTCGTGTTGTCGAATATGTTTGTCGAAAATGTTGGTATGCTTAAAATAATAAACGCGACACAAAAGGCACAGGAt                                                                         <  3:25969/133‑1 (MQ=255)
                                                            aCAAACTGTCGCATAAGTCTTAATTTATGCTACAGCCCGATCTCTCGTGATTTTGCTGGGTGCGGGTTGTCGAATAAGTTTGTCGAAAATGGTGGTATGATTAAAACAATAAACGCGACACAAAAGGCACAGGAt                                                                         <  3:175470/135‑1 (MQ=255)
                                                            aCAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAATAATAAACGCGACACAAAAGGCACAGGAt                                                                         <  2:65563/135‑1 (MQ=255)
                                                                aCTTTCGCATTAGTCTTATGTTATGGTACAGCCCTATCTCTGGTTTTTTTGCTTGGTTGGGGTTGTGTCTTAAGTTTGTCGCAAATGGTGGTTTGATTAAAAGTATAACCGCGACACAAAAGGCACAGGATTATGa                                                                    <  3:88831/136‑1 (MQ=255)
                                                                  tGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGaa                                                                   >  3:156474/1‑135 (MQ=255)
                                                                    tCGCATAAGTCTTAAGTTATGCTACAGCCCGATCTCTCGTTATTTTGCTGGGTGCGGGTTGTCGAATAAGTTTGTCGCAAATGGTGGTATGCTTAAAACAATAAACGCGACACAAAAGGCACAGGATTATGAata                                                                 <  6:60748/135‑1 (MQ=255)
                                                                      gCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTg                                                              >  5:158424/1‑136 (MQ=255)
                                                                       cATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACTCGACACAAAAGGCACAGGATTATGAATATTg                                                              >  6:236811/1‑135 (MQ=255)
                                                                       cATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTg                                                              >  5:254079/1‑135 (MQ=255)
                                                                       cATAACTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCTGGTTGTCGCATAAGTTTGTCGCAAATGCTGGAATGCTTAACAGAATAAACGCGACACAACAGGCACAGGATTATGAATATTg                                                              >  8:204783/1‑135 (MQ=255)
                                                                         tAAGTCTTAATTTATGCGACATCCCGATCTCTCTTGATTTTGATGGTTTCGGGTTGTCGAATAAGTTTGTCGCAATTGGTGGTATTCTTAAAATAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGat                                                           <  5:282054/136‑1 (MQ=255)
                                                                            gTCTTAAGTTTTGCTAAAGCCCTATCTCTGGTGTTTTTGCTGGGTTGGGGTTTTCGAATAAGTTTGTCGCAAATGGTGGTATGCTTAAAATAATAACCGCGACACAAAAGGCACAGGATTATGAATATTGGATATg                                                        <  2:265704/136‑1 (MQ=255)
                                                                               ttAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGc                                                       >  7:110880/1‑134 (MQ=255)
                                                                                 ttgTTATGCCACTGCCCTATGTCTGGTTATTTTGCTGGGTTCGGGTTGTCGAATATGTTTGTCGCAATTGGTGGTATGCTTAAAATAATAACCGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCg                                                      <  4:123127/131‑1 (MQ=255)
                                                                                   gTTATGCTACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGTGGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGt                                                 <  3:282369/136‑1 (MQ=255)
                                                                                   gTTATGCGACAGCCCGATCTCTCGTTATTTTGCTGGGTGCGGGTGGTCGCATAAGTTTGGCGAAAATGGTGTTATGCTAAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGt                                                 <  7:166763/136‑1 (MQ=255)
                                                                                   gTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAACCGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGt                                                 <  1:187159/136‑1 (MQ=255)
                                                                                    ttATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTa                                                >  4:250691/1‑136 (MQ=255)
                                                                                       tGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGGATAAGTTTGTCGCTAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATc                                              >  3:20692/1‑135 (MQ=255)
                                                                                         cgaatgCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGAATAAGTTTGTCTCAAATGGTGGTTTGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGa                                            <  7:208705/130‑1 (MQ=255)
                                                                                         cGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGATTATTGGATATGCGAGAGTATCGAc                                           >  6:73108/1‑136 (MQ=255)
                                                                                         cGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGAc                                           >  2:123803/1‑136 (MQ=255)
                                                                                          gACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGAcc                                          >  2:242427/1‑136 (MQ=255)
                                                                                          gACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGAcc                                          >  7:131799/1‑136 (MQ=255)
                                                                                          gACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGAcc                                          >  6:108412/1‑136 (MQ=255)
                                                                                          gACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGAcc                                          >  7:22861/1‑136 (MQ=255)
                                                                                          gACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGAcc                                          >  7:271526/1‑136 (MQ=255)
                                                                                          gACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGAcc                                          >  5:89166/1‑136 (MQ=255)
                                                                                          gACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGAcc                                          >  4:173339/1‑136 (MQ=255)
                                                                                          gACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATACTGGATATGCGAGAGTATCGAcc                                          >  6:156273/1‑136 (MQ=255)
                                                                                            cAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCTCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAATAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTc                                        <  1:182852/136‑1 (MQ=255)
                                                                                             aGCCCGAGATCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGGGGTATGCTTAAAAGAAAAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTcc                                       <  6:214453/136‑1 (MQ=255)
                                                                                              gCCCGATCTCTCTTGATTTTGCTGGGTTCGGGTTGTCGAATAAGTTTGTCGCAATTGGTGGTATGCTTAAAAGAATAACCGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCg                                      <  1:174368/136‑1 (MQ=255)
                                                                                              gCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGAATAAGTTTGTCGCAAATGGTGGTATGCTTAAAACAAAAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCg                                      <  8:283739/136‑1 (MQ=255)
                                                                                                  gATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCTACCTCCGGa                                    >  6:31910/1‑134 (MQ=255)
                                                                                                  gATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCAGAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGa                                    >  4:80174/1‑134 (MQ=255)
                                                                                                    gctctcGTTATTTTGCTGGGTTCGGTTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAAAAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAgg                                <  3:56739/135‑1 (MQ=255)
                                                                                                      tctcGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGAc                              >  6:89977/1‑136 (MQ=255)
                                                                                                        tcGTGATTTTGGTGGGTTCGGGTTGTCGCTTAAGTTTGTCGCAATTGGTGGTATGATTAAAAGAAAAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAgg                                <  1:147228/132‑1 (MQ=255)
                                                                                                           tGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGAATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGa                         <  3:121878/136‑1 (MQ=255)
                                                                                                            gATTTTGCTGGGTTCGGGTTGTCGCATAATTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACTAGACACAAAAGGAACAGGATTATGAATATTGGATATGCGAGAGTATCGACCGCCGGACAGGACTTGGAg                        >  3:246138/1‑136 (MQ=255)
                                                                                                            gATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAg                        >  7:17747/1‑136 (MQ=255)
                                                                                                            gATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAg                        >  8:118400/1‑136 (MQ=255)
                                                                                                            gATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAg                        >  3:22294/1‑136 (MQ=255)
                                                                                                             aTTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAg                        <  2:214879/135‑1 (MQ=255)
                                                                                                                   gtGGGTTCGGGTTGTCTAATATGTTTGTCGCAAATGGTGGTATGCATAAAACAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAGTTACAAc                 <  1:112240/135‑1 (MQ=255)
                                                                                                                            gtttGTCGAATTATTTTTTCCCAATTGGTTTTATCCTAAAAAGAATAACCGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAGTTACAACGTCAGCTAt        <  5:236811/134‑1 (MQ=255)
                                                                                                                            ggtggtCGCATAAGTTTGTCGCAAATGGGGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAGTTACAACGTCAGCTAt        <  7:201076/136‑1 (MQ=255)
                                                                                                                            ggTTGTCTCATATGTTTGTCCCAAATGGTGGTATGCTTAAAACAATAACCGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAGTTACAACGTCAGCTAt        <  4:22201/136‑1 (MQ=255)
                                                                                                                            ggTTGTCGCATAAGTTTGTCGAAAATGGTGTTATGCTTAAAATAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAGTTACAACGTCAGCTAt        <  6:80883/136‑1 (MQ=255)
                                                                                                                            ggTTGTCGAATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAGTTACAACGTCAGCTAt        <  7:255415/136‑1 (MQ=255)
                                                                                                                              ttGTCGAATAAGTTTGTCGAAATTGGTGTTATGATTAAAACAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAGTTACAACGTCAGCTATTg      <  8:148639/136‑1 (MQ=255)
                                                                                                                               tGTCGAATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAGTTACAACGTCAGCTATTg      <  6:249798/135‑1 (MQ=255)
                                                                                                                                  cGCATAAGTTTGTCGCAAATGGTGGTTTGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAGTTACAACGTCAGCTATTGACGc  <  7:44722/136‑1 (MQ=255)
                                                                                                                                   gCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGa                                            >  1:243759/1‑93 (MQ=255)
                                                                                                                                   gCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAGTTACAACGTCAGCTATTGACg   >  4:201539/1‑134 (MQ=255)
                                                                                                                                    |                                                                                                                                     
ACTACACCTACAAATTCAATTGATCAAAATTGGGTAAGCGGTACGTCAGGTTGTATGTTTACAAACTGTCGCATAAGTCTTAAGTTATGCGACAGCCCGATCTCTCGTGATTTTGCTGGGTTCGGGTTGTCGCATAAGTTTGTCGCAAATGGTGGTATGCTTAAAAGAATAAACGCGACACAAAAGGCACAGGATTATGAATATTGGATATGCGAGAGTATCGACCTCCGGACAGGACTTGGAGTTACAACGTCAGCTATTGACGC  >  ACB143/2176804‑2177069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: