| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 2,847,248 | A→G | 100% | intergenic (+20/‑26) | CJ019_02702 → / → CJ019_02703 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 2,847,248 | 0 | A | G | 93.6% | 97.8 / ‑1.2 | 31 | intergenic (+20/‑26) | CJ019_02702/CJ019_02703 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (19/10); total (20/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTTCGGCTACTGGATCCGCCAGGAAGGGTTGCAACTGACCCATCTGCTGTTGATGGTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGAGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCTTTGCTCGAAGGGCTCGGCAAGCCAGGGGACACGCTGATCGGCGTATCACGCAAACAGC > ACB143/2847125‑2847376 | ggTTCGGCTACTGGATCCGCCAGGAAGGGTTGCAACTGACCCATCTGCTGTTGATGGTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGtt > 2:52826/1‑135 (MQ=255) tACTGGATCCGCCAGGAAGGGTTGCAACTGACCCATCTGCTGTTGATGGTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGAGCATTTCAGTTAAAAGGAg > 6:120439/1‑135 (MQ=255) tACTGGATCCGCCAGGAAGGGTTGCAACTGACCCATCTGCTGTTGATGGTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCGTGGGGCATTTCAGTTAAAAGGAg > 7:254210/1‑135 (MQ=255) gTTGCAACTGACCCATCTGCTGTTGATGGTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATc < 5:96027/136‑1 (MQ=255) cAACTGACCCATCTGCTGTTGATGGTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGAGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAg < 1:292117/136‑1 (MQ=255) aCCCATCTGCTGTTGATGGTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCg < 7:269088/136‑1 (MQ=255) cATCTGCTGTTGATGGTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGAGGAAAATGATGATCGTAGGCGCCgg > 6:243977/1‑134 (MQ=255) gctgTTGATGGTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAat < 1:40767/111‑1 (MQ=255) gctgTTGATGGTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAat > 2:40767/1‑111 (MQ=255) gctgTTGATGGTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAggg > 3:50476/1‑136 (MQ=255) ttGATTGTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGtt < 6:272790/134‑1 (MQ=255) aTGGTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGcc > 3:183365/1‑136 (MQ=255) gTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGATAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCgg < 5:128065/135‑1 (MQ=255) ggATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCttt > 1:260831/1‑136 (MQ=255) ggATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCGTGGGGCATTTCATTTTAAATGAAGAACGGAAGAAAATGAATCACGTACGCCCCGGCAGCAGGTTTGGCCGGGCatt > 4:29894/1‑136 (MQ=255) gATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCTTTg > 3:147665/1‑136 (MQ=255) gATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGCTTGGGCCGGGCTTTg > 1:239713/1‑136 (MQ=255) tCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCTTTGCt > 8:242962/1‑136 (MQ=255) ctgcACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCTTTGCTCGaa < 8:204446/135‑1 (MQ=255) tgcACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCTTTGCTCGAAgg > 4:106840/1‑136 (MQ=255) tgcACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCTTTGCTCGAAgg > 8:213657/1‑136 (MQ=255) tgcACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGAGCCGGCAGAGGGTTGGGACGGGCTTTGCTCGAAgg > 7:179944/1‑136 (MQ=255) cgtACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCTTTGCTCGAAGGGCTCGGCAAg > 3:64755/1‑136 (MQ=255) tGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCTTTGCTCGAAGGGCTCGGCAAGCCAgg > 5:281752/1‑136 (MQ=255) gTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCTTTGCTCGAAGGGCTCGGCAAGCCAGGGGac > 4:219168/1‑135 (MQ=255) gTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCTTTGCTCGAAGGGCTCGGCAAGCCAGGGGac < 3:219168/135‑1 (MQ=255) gCTGGAGTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCACCGGATGAAAATGATGATCGTACGCGCCGGCAGAGGGTTGGGCCGGGCTTTGCTCGAAGGGCGCGGCAAGCCAGGGGACACGCt < 1:230024/136‑1 (MQ=255) gTTGAACGGCCCAGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCTTTGCTCGAAGGGCTCGGCAAGCCAGGGGACACGCTGATCgg > 2:253041/1‑136 (MQ=255) aGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCTTTGCTCGAAGGGCTCGGCAAGCCAGGGGACACGCTGATCGGCGTATCACGCaa < 4:64755/136‑1 (MQ=255) aGGGAAGCTTGGGGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCTTTGAGCGAAGGGCTCGGCAAGCCAGGGGACACGCTGATCGGCGTATCCCGCa > 4:192977/1‑135 (MQ=255) aGCTTGGGGCATTTCAGATAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGGTGGGCCGGGCTTTGCTCGAAGGGCTCGGCAAGCCAGGGGACACGCTGATCGGCGTATCACGCAAACAGc < 3:124745/136‑1 (MQ=255) | GGTTCGGCTACTGGATCCGCCAGGAAGGGTTGCAACTGACCCATCTGCTGTTGATGGTCTGGATCCTGCTGCACTTCGTCGTACTGAATGTCCGCTGGAGTTGAACGGCCCAGGGAAGCTTGGAGCATTTCAGTTAAAAGGAGCAACGGATGAAAATGATGATCGTAGGCGCCGGCAGAGGGTTGGGCCGGGCTTTGCTCGAAGGGCTCGGCAAGCCAGGGGACACGCTGATCGGCGTATCACGCAAACAGC > ACB143/2847125‑2847376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |