Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 195,703 C→T 6.2% S3521L (TCG→TTG)  CJ019_00185 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143195,7030CT 6.2% 205.6 / 3.9 64S3521L (TCG→TTG) CJ019_00185hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (22/38);  new base T (2/2);  total (24/40)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCC  >  ACB143/195568‑195834
                                                                                                                                       |                                                                                                                                   
gcggcgCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTc                                                                                                                                     >  1:292016/1‑136 (MQ=255)
       aGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGcc                                                                                                                              <  5:205275/136‑1 (MQ=255)
            cGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGaa                                                                                                                         <  2:166340/136‑1 (MQ=255)
                 aaGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTc                                                                                                                     >  2:88909/1‑135 (MQ=255)
                  aGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCaa                                                                                                                   <  1:245830/136‑1 (MQ=255)
                  aGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCaa                                                                                                                   <  6:87532/136‑1 (MQ=255)
                   gTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCaaa                                                                                                                  <  3:121769/136‑1 (MQ=255)
                      cGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAAcc                                                                                                                <  8:111482/135‑1 (MQ=255)
                      cGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAAcc                                                                                                                <  4:95820/135‑1 (MQ=255)
                        aaaaCCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGAc                                                                                                             <  8:126089/136‑1 (MQ=255)
                          aaCCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCt                                                                                                           <  8:260077/136‑1 (MQ=255)
                             atGAATCTGGACAAGACCCCTGTCAGCCCCGAGGTCACTGACGAGCCAGGCACGACAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCATACCTCGGTGGCCGAGAACGTCAAACCGACCTTCa                                                                                                        <  1:72395/135‑1 (MQ=17)
                                aaTCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCAcc                                                                                                      <  7:140808/135‑1 (MQ=255)
                                aaTCTGGACAAGACCCCGGTCAGCACCGAGGTCACAGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCAcc                                                                                                      <  5:197445/135‑1 (MQ=255)
                                aaTCTGGACAAAACCCCTGTCAGCACCGAGGTCACTGACGATCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCAcc                                                                                                      <  5:203917/135‑1 (MQ=255)
                                         aaGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGTCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCa                                                                                            <  4:85009/136‑1 (MQ=255)
                                         aaGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCa                                                                                            <  4:35602/136‑1 (MQ=255)
                                         aaGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCa                                                                                            <  1:128903/136‑1 (MQ=255)
                                         aaGACCCCGGGCAGCACCGAGTTAACTGACGAGCCAGGAACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCa                                                                                            <  6:34337/136‑1 (MQ=255)
                                         aaGACCCCGGGCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCa                                                                                            <  6:37784/136‑1 (MQ=255)
                                              cccGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACAcc                                                                                       >  6:161776/1‑136 (MQ=255)
                                               ccGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCg                                                                                      <  8:188540/136‑1 (MQ=255)
                                                      gCACCGAGGTCACTGACGAGCCAGGCACTCCAGTCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGc                                                                               <  7:273895/136‑1 (MQ=255)
                                                      gCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGc                                                                               <  3:199052/136‑1 (MQ=255)
                                                      gCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGc                                                                               <  7:277701/136‑1 (MQ=255)
                                                        aCCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGcc                                                                              >  4:282385/1‑135 (MQ=255)
                                                            aGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGa                                                                         <  4:171686/136‑1 (MQ=255)
                                                               tCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCa                                                                                            <  6:80208/114‑1 (MQ=255)
                                                               tCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCa                                                                                            >  5:80208/1‑114 (MQ=255)
                                                               tCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACAAATCAACACCGCCTTGGCCCACGACCt                                                                      >  2:202823/1‑136 (MQ=255)
                                                                 aCTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTgg                                                                    <  5:210790/136‑1 (MQ=255)
                                                                      cGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTg                                                               <  3:168968/136‑1 (MQ=255)
                                                                      cGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTg                                                               <  1:170047/136‑1 (MQ=255)
                                                                           cAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCt                                                          >  2:101794/1‑136 (MQ=255)
                                                                           cAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCt                                                          >  1:232856/1‑136 (MQ=255)
                                                                           cAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGGCACTATTACTGCGGACCAGCCCTCGGTGGCCGAGATCGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGccccg                                                          >  7:215894/1‑135 (MQ=18)
                                                                                       gCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGc                                              >  1:71058/1‑136 (MQ=255)
                                                                                          aCGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCa                                           <  4:132173/136‑1 (MQ=255)
                                                                                          aCGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCa                                           <  6:78033/136‑1 (MQ=255)
                                                                                            gAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAgg                                         <  4:106076/136‑1 (MQ=255)
                                                                                              aGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCCCCTTGGCCCACGACCTGGTCGTGACCAGGACCAACACGCCCCAGGTc                                       >  2:233878/1‑136 (MQ=18)
                                                                                               ggCGATATCTTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAAAAGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTCGTCGTGACCCTGAGCCACAACGCCCAGGTCa                                      >  6:208536/1‑136 (MQ=18)
                                                                                               ggCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTcc                                      >  1:115426/1‑135 (MQ=255)
                                                                                               ggCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCa                                      >  8:43578/1‑136 (MQ=255)
                                                                                                      tCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATc                                 >  2:250603/1‑134 (MQ=255)
                                                                                                      tCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACAGATCAACCCCGCCATGGCCCACGACCTGGTCGTGACCCTGAGCAACAAAGCCCAGGTCCCCATCaa                               >  8:128852/1‑136 (MQ=18)
                                                                                                            aaGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCgg                         <  1:158758/136‑1 (MQ=255)
                                                                                                             aGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGc                        >  4:18150/1‑136 (MQ=255)
                                                                                                                    aTTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACGTATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCAGAGCAACAACGCCCAGGTAACCATCAATGCCGGCGAAACCa                 >  3:102099/1‑136 (MQ=255)
                                                                                                                     ttACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCa                 <  6:101890/135‑1 (MQ=255)
                                                                                                                           ctgACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAgcgcg            <  3:18150/134‑1 (MQ=255)
                                                                                                                           ctgACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAgcgcg            <  7:28886/134‑1 (MQ=255)
                                                                                                                           ctgACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGcc          >  2:40068/1‑136 (MQ=255)
                                                                                                                            tgACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGcc          >  4:270782/1‑135 (MQ=255)
                                                                                                                              cccAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGCCCCTGCGCAACAACGCCCAGGTCACCATCAAGGCCGGCCAAACCAGCGCGCCGtc       >  6:93767/2‑135 (MQ=255)
                                                                                                                              aCCAGACTTCGGTAGCCGACAACGTCAACCCCACCTTCACCGTCCAAATCAACCCCCCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTa       <  2:293921/136‑1 (MQ=11)
                                                                                                                              aCCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACTGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTa       <  3:258340/136‑1 (MQ=255)
                                                                                                                              aCCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTGACCATCAAGGCCGGGGCACCCAGCGCGCCgga       >  6:9957/1‑134 (MQ=17)
                                                                                                                                cAGACCTTGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCa                 >  7:121847/1‑124 (MQ=255)
                                                                                                                                cAGACCTTGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCa                 >  7:117903/1‑124 (MQ=255)
                                                                                                                                cAGACCTTGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCa                 <  8:117903/124‑1 (MQ=255)
                                                                                                                                cAGACCTTGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCa                 <  8:121847/124‑1 (MQ=255)
                                                                                                                                cAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCAcc                                                                                                      >  5:112850/1‑39 (MQ=33)
                                                                                                                                   agcTCGGTGGCCGAGATCGTCAAACCGACCTTCACCGTACATATCAACTCCGCCTTGGCCCTCGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACAccc  <  8:127833/134‑1 (MQ=255)
                                                                                                                                   aCCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAAGACCGCCTTGCCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACAccc  <  6:262206/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
GCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACATATCAACACCGCCTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCC  >  ACB143/195568‑195834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: