Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 3,615,019 T→C 7.3% H20R (CAC→CGC)  dauR_2 ← Transcriptional regulator DauR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,615,0190TC 7.3% 168.9 / 3.6 55H20R (CAC→CGC) dauR_2Transcriptional regulator DauR
Reads supporting (aligned to +/- strand):  ref base T (18/33);  new base C (2/2);  total (20/35)
Fisher's exact test for biased strand distribution p-value = 6.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TTGGTACCCTTCAAAAGGTCCCCCAGTTCTGGACCGAGTGCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTATGGTGGCCCTGGAGAATACTGGGCACAAGACGGATCTTTGCCG  >  ACB143/3614890‑3615152
                                                                                                                                 |                                                                                                                                     
ttGGTACCCTTCAAAAGGTCCCCCAGTTCTGGACCGAGTGCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTggggg                                                                                                                                  >  4:214146/1‑135 (MQ=255)
     aCCCTTCAAAAGGTCCCCCAGTTCTGGACCGAGTGCCGAATCATCGCCGCGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTAcagc                                                                                                                            >  3:206864/1‑136 (MQ=255)
      cccTTCAAAAGGTCCCCCAGTTCTGGACCGAGTGCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTAcag                                                                                                                             >  6:260565/1‑134 (MQ=255)
            aaaaGGTCCCCCAGTTCTGGACCGAGTGCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAggg                                                                                                                     >  4:232312/1‑136 (MQ=255)
            aaaaGGTCCCCCAGTTCTGGACCGAGTGCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAggg                                                                                                                     >  1:227700/1‑136 (MQ=255)
            aaaaGGTCCCCCAGTTCTGGACCGAGTGCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAggg                                                                                                                     <  7:35695/136‑1 (MQ=255)
            aaaaGGTCCCCCAGTTCTGGACCGAGTGCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAggg                                                                                                                     >  5:216084/1‑136 (MQ=255)
            aaaaGGTCCCCCAGTTCTGGACCGAGTGCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAggg                                                                                                                     >  2:42295/1‑136 (MQ=255)
                      ccAGTTCTGGACCGAGTGCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCttt                                                                                                            >  2:242327/1‑135 (MQ=255)
                        acttCTGTACCGAGTGCCGAATCATCGACTAGCTTGCGATCTGAGAAATTATTGGCGATATGTACGATTGTGTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTAAAGCAGCAGGGCAATGCTTTCAg                                                                                                         <  3:200927/134‑1 (MQ=255)
                        aGTTCTGGACCGAGTGCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAg                                                                                                         <  6:216084/136‑1 (MQ=255)
                                  cGAGTGCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTg                                                                                               >  3:200135/1‑136 (MQ=255)
                                      tGCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGTCAATGCTTTCAGCGATTGCCTGGTaa                                                                                           >  1:121339/1‑136 (MQ=255)
                                       gCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAAt                                                                                          >  4:96510/1‑136 (MQ=255)
                                       gCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGCGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAAt                                                                                          >  7:39548/1‑136 (MQ=255)
                                       gCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGCGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAAt                                                                                          >  7:43603/1‑136 (MQ=255)
                                          gAATCATCGCCGAGGTTGCGATGTGAGAAATTTTTGGCGATATGAACTATTGTGTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAAtttt                                                                                       <  4:226930/136‑1 (MQ=255)
                                          gAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAAtttt                                                                                       <  6:42795/136‑1 (MQ=255)
                                          gAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAAtttt                                                                                       <  7:135466/136‑1 (MQ=255)
                                          gAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAAtttt                                                                                       <  8:3233/136‑1 (MQ=255)
                                               atcGTCTAGCTTGAGATGTTAGAATTTATTTGGGATATGAACGATTGTCTGACTGAGTATCTGATGAATGACGTCTTCATGGTTGTTGTTCAGCAGCAGGGCATTGCTTTCAGCGATTGCCTTGTAATTTTCCAgt                                                                                  <  2:127548/136‑1 (MQ=255)
                                               atcGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAgt                                                                                  <  1:191564/136‑1 (MQ=255)
                                               atcGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAgt                                                                                  <  5:176921/136‑1 (MQ=255)
                                               atcGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAgt                                                                                  <  2:47721/136‑1 (MQ=255)
                                               atcGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAgt                                                                                  <  2:121339/136‑1 (MQ=255)
                                                   ccGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCa                                                                              >  8:224146/1‑136 (MQ=255)
                                                    cGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCAt                                                                             <  5:35605/136‑1 (MQ=255)
                                                                 tgAGAAATTATTGGCGATATGCACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAAt                                                                 <  8:265804/135‑1 (MQ=255)
                                                                 tgAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAAt                                                                 <  2:48889/135‑1 (MQ=255)
                                                                 tgAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGCAATTTTCCAGTGTCATGCATTAGCCAAt                                                                 <  4:189061/135‑1 (MQ=255)
                                                                          cttGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTg                                                        <  2:111238/134‑1 (MQ=255)
                                                                           ttGGCGATATGAACGATTGTCTGACTGCGGATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTga                                                       <  2:138962/135‑1 (MQ=255)
                                                                              gCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTggggg                                                                                                                                  >  3:266477/1‑57 (MQ=255)
                                                                              gCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTggggg                                                                                                                                  <  4:266477/57‑1 (MQ=255)
                                                                                gATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATg                                                 >  1:285038/1‑136 (MQ=255)
                                                                                gATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATg                                                 >  3:234009/1‑136 (MQ=255)
                                                                                    tGAACTATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTa                                             <  6:174237/136‑1 (MQ=255)
                                                                                    tGAACGTTTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTa                                             <  8:11118/136‑1 (MQ=255)
                                                                                      aaCGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTAtg                                           >  6:262146/1‑136 (MQ=255)
                                                                                         gATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTAtggtg                                        <  4:125348/136‑1 (MQ=255)
                                                                                         gATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTAtggtg                                        <  2:37677/136‑1 (MQ=255)
                                                                                         gATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTAtggtg                                        <  6:202536/136‑1 (MQ=255)
                                                                                         gATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTAtggtg                                        <  6:146796/136‑1 (MQ=255)
                                                                                         gATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTAtggtg                                        <  3:142191/136‑1 (MQ=255)
                                                                                         gATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTAtggtg                                        <  3:273817/136‑1 (MQ=255)
                                                                                             gTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTATGGTGGcc                                     <  3:96510/135‑1 (MQ=255)
                                                                                              tCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTATGGTGGCCCt                                   <  2:161175/136‑1 (MQ=255)
                                                                                                   cTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTATGGTGGCCCTGgaga                              <  5:77609/136‑1 (MQ=255)
                                                                                                      cGTATCTCATGAATGACGACTTCCGCGCGGTGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTATGGTGGCCCTGGAGAAt                            <  8:43603/135‑1 (MQ=255)
                                                                                                      cGTATCTCATGAATGACGACTTCCGCGCGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTATGGTGGCCCTGGAGAAt                            <  8:39548/135‑1 (MQ=255)
                                                                                                         aTCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTATGGTGGCCCTGGAGAATACTg                        >  5:32737/1‑136 (MQ=255)
                                                                                                              atgaatgaCGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTATGGTGGCCCTGGAGAATACTgg                       <  2:139059/132‑1 (MQ=255)
                                                                                                                        acTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTATGGTGGCCCTGGAGAATACTGGGCACAAGACGGATc         >  5:262348/1‑136 (MQ=255)
                                                                                                                               cgTGGGGGTACAGCAGCAGGGCAATGCTTTCATCGATTGCATGGTTATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTATGGTGGCCCTGGAGAATACTGGGCACAAGACGGATCTTTGCCg  <  2:32326/136‑1 (MQ=255)
                                                                                                                               cgTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTATGGTGGCCCTGGAGAATACTGGGCACAAGACGGATCTTTGCCg  <  5:260565/136‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                     
TTGGTACCCTTCAAAAGGTCCCCCAGTTCTGGACCGAGTGCCGAATCATCGCCGAGCTTGCGATGTGAGAAATTATTGGCGATATGAACGATTGTCTGACTGCGTATCTCATGAATGACGACTTCCGCGTGGGGGTACAGCAGCAGGGCAATGCTTTCAGCGATTGCCTGGTAATTTTCCAGTGTCATGCATTAGCCAATGGTCTGTTGAAGAATGCCTATGGTGGCCCTGGAGAATACTGGGCACAAGACGGATCTTTGCCG  >  ACB143/3614890‑3615152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑