Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,807,160 G→C 11.6% E70Q (GAA→CAA)  mcbC → Microcin B17‑processing protein McbC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,807,1600GC11.6% 403.8 / 6.0 149E70Q (GAA→CAA) mcbCMicrocin B17‑processing protein McbC
Reads supporting (aligned to +/- strand):  ref base G (83/44);  new base C (2/17);  total (86/63)
Fisher's exact test for biased strand distribution p-value = 6.33e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

CAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACTAAAAGCCCTAC  >  ACB143/4807027‑4807293
                                                                                                                                     |                                                                                                                                     
cAAAGGAAAATTAACGATACAAAAATCAACTCAAAATTTGACCGCCCTGCACCAAATACCACAAAAAGACCTCGAAAAACTAACCGCAAACGACCTAAAACTAAATACCATTGTAGCCCAACGAAAAAACAAAGac                                                                                                                                     <  6:66513/136‑2 (MQ=255)
     taaaCTATACGATAACCAAACCAACTCAACACTTGAGCGCCCTGCACAAAATACCACCAAAACACCTCTAAAAACTTACCGCAAACGAACTAAAAATCAATACCATTGTCCCCCCACGCAAAAACAAACACCCGAc                                                                                                                                <  4:205387/134‑1 (MQ=255)
     gCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGAc                                                                                                                                >  5:189534/1‑136 (MQ=255)
       aaCTATACTAAACACAAAGCAACACAACACTTGAGCCCCCTCCACCAAAAACCACCAAACGACCTCGAAAAACTTACCGCAAACCAACAAAAACTCAAAACCATTGTACCCCCAACCAAAAACAAACACCCGActc                                                                                                                              <  6:272278/136‑1 (MQ=255)
       aaCTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAACAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGActc                                                                                                                              <  2:221778/136‑1 (MQ=255)
                    acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGc                                                                                                                 >  8:82045/1‑136 (MQ=255)
                    acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGc                                                                                                                 >  5:124363/1‑136 (MQ=255)
                    acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGc                                                                                                                 >  5:258877/1‑136 (MQ=255)
                    acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGc                                                                                                                 >  6:133817/1‑136 (MQ=255)
                    acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGc                                                                                                                 >  7:216929/1‑136 (MQ=255)
                    acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTAAACCTCTGGc                                                                                                                 >  1:18517/1‑136 (MQ=255)
                    acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACAGACTCTTACACCTCTGGc                                                                                                                 >  5:292541/1‑136 (MQ=255)
                      aaaGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGc                                                                                                                 >  6:159721/1‑134 (MQ=255)
                       aaGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAg                                                                                                              >  3:194405/1‑136 (MQ=255)
                       aaGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAg                                                                                                              >  1:100834/1‑136 (MQ=255)
                       aaGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAg                                                                                                              >  2:43613/1‑136 (MQ=255)
                       aaGCAACTCAACACTTGAGCGCCCTGCACCAAATACAACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAg                                                                                                              >  8:159834/1‑136 (MQ=255)
                         gCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGcc                                                                                                            >  1:178767/1‑136 (MQ=255)
                           aacacaacacTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCca                                                                                                          >  2:33870/1‑136 (MQ=255)
                           aaCTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTAACGGAAACGAACTAAAACTCAATACCATTGTAGCCCCACGCAAAAACCAAGAACCGACTCTTACACCTCTGGCGAGACca                                                                                                          >  8:267719/1‑136 (MQ=255)
                           aaCTCAACACTAGCGCGCCCTGCACCAAATACAACCAAAAGACCTCGAAAAACTTAACGGAAACGAACTAAAACTCAATACCATTCTCGCCCCAAGCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGcccc                                                                                                          >  4:17822/1‑135 (MQ=255)
                            aCTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAACACCTCGAAAAACTTCCCGTAAACCAACTAAAACTCAATCCCATTGTACCCCCAACCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCcac                                                                                                         <  4:172815/136‑1 (MQ=255)
                               cAACACTTGAGCGCCCTCCACAAAAAACCACCAACAGACCTCAAAAAAATTACCGAAACCCAAAAAAAACACAAACCCATTCTACCCCCACCCAAAAAAAAACACCCGACTCTTACACCTCTGGCGAGCCCACAcc                                                                                                      <  2:142166/136‑1 (MQ=255)
                                aaCACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCCCAccc                                                                                                     >  2:60976/1‑136 (MQ=255)
                                aaCACTTGAGCGCCCTCCACCAAATACCACCAAAAGACCTCGAAAATCTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGAAGCTTACACCTCTGGCGAGCCCACAccc                                                                                                     >  8:214035/1‑136 (MQ=255)
                                   acTTTAGCGCCCTGCACAAAATACCACCAAAACACCTCAAAAAACTTACCTTAAACCAACAAAAACTAAATACCATTGTACCCCCAACCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCt                                                                                                  <  1:135928/136‑1 (MQ=255)
                                          cgcCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACgaag                                                                                            >  2:43453/1‑135 (MQ=255)
                                           gcCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACgaaga                                                                                           >  3:33959/1‑135 (MQ=255)
                                           gcCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAAAAACATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACgaaga                                                                                           >  3:75357/1‑135 (MQ=255)
                                                  aCCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGCGCCCACACCCGCTACGAAGAAATAGtt                                                                                    >  2:101161/1‑135 (MQ=255)
                                                  aCCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGtt                                                                                    >  6:223521/1‑135 (MQ=255)
                                                      aaTACCACCAAACCACCTCGAAAAAATTACCCTAAACGACATAAAACTCAATACCATTTTAGCCCCAACAAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGc                                                                               <  8:151012/136‑1 (MQ=255)
                                                      aaTACCACCAAAAGACCTCGAAAAACTTCCCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCACGCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGc                                                                               <  1:93032/136‑1 (MQ=255)
                                                      aaTACCACCAAAACACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGAAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGc                                                                               <  8:69113/136‑1 (MQ=255)
                                                         accaccAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACCCCCGCTACGAAGAAATAGTGCATGCGaa                                                                            >  4:2815/1‑136 (MQ=255)
                                                              caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAtt                                                                       >  3:140959/1‑136 (MQ=255)
                                                              caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAtt                                                                       >  3:111066/1‑136 (MQ=255)
                                                              caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAtt                                                                       >  7:93913/1‑136 (MQ=255)
                                                              caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAtt                                                                       >  1:65524/1‑136 (MQ=255)
                                                              caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCACAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAtt                                                                       >  7:11561/1‑136 (MQ=255)
                                                              caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACAATTCTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTAATGAGAACGAtg                                                                       >  8:242655/1‑135 (MQ=255)
                                                              caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAAAACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAtt                                                                       >  4:247206/1‑136 (MQ=255)
                                                              caAAACACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAACCAAAAACAAATAACCCACTATTACACATCAGGAGAGCCCACAACCGCTACGAAGACATAGTTCATCCGAAAGAt                                                                        >  4:11744/1‑135 (MQ=255)
                                                                   gACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAg                                                                  >  3:161080/1‑136 (MQ=255)
                                                                   gACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAg                                                                  >  3:181064/1‑136 (MQ=255)
                                                                   gACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAg                                                                  >  4:76189/1‑136 (MQ=255)
                                                                   gACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACAACCGCTACGAAGAAATAGATCATGCGAACGAATTCAAg                                                                  >  4:227737/1‑136 (MQ=255)
                                                                   gACCTCCAAAAACTTCCCGGAAACGAACTAAAACTCAATCCCTTTGTAGCCCCAACCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAg                                                                  <  1:60919/136‑1 (MQ=255)
                                                                      cTCGAAAAACTTACCGGAAACTAACTAAAACTCAATACCATTGTAGCCCCAACCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCAc                                                               <  8:44634/136‑1 (MQ=255)
                                                                        cGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGc                                                             >  6:18138/1‑136 (MQ=255)
                                                                        cGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGc                                                             >  5:239799/1‑136 (MQ=255)
                                                                        cGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGc                                                             >  6:238038/1‑136 (MQ=255)
                                                                         gAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGcc                                                            >  1:10558/1‑136 (MQ=255)
                                                                           aaaaCTTACCGGACACCAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                                          <  2:35469/136‑1 (MQ=255)
                                                                           aaaaCTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGCGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                                          >  6:87042/1‑136 (MQ=255)
                                                                           aaaaCTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                                          <  3:16966/136‑1 (MQ=255)
                                                                           aaaaCTTACCGCAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                                          <  1:38233/136‑1 (MQ=255)
                                                                            aaaCTTCCCGGAAACGAACTAAAACTCAATACCTTTGTAGCCCCAACCAAAAACAACGACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCctc                                                         <  3:105919/136‑1 (MQ=255)
                                                                                    cGGAAACGAACTAAAACTCAATACCATTGTAGCCCCACCCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACttt                                                 <  4:246803/136‑1 (MQ=255)
                                                                                    cGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACtat                                                 >  2:84891/1‑134 (MQ=255)
                                                                                     ggAAACGAACTAAAACTCAATACCTTTGTAGCCCCAAGAAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                                <  7:32834/136‑1 (MQ=255)
                                                                                     ggAAACGAACTAAAACTCAATACCATTGTCGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                                <  4:87632/136‑1 (MQ=255)
                                                                                     ggAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                                <  2:246956/136‑1 (MQ=255)
                                                                                     ggAAACGAACTAAAACTCAATACCATTGTAGCCCCAACCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                                <  5:55037/136‑1 (MQ=255)
                                                                                     gcaaaCTAACTAAAACTCAATACCATTGTAGCCCCAAGAAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                                <  5:172474/134‑1 (MQ=255)
                                                                                      gAACCGAACTAAAACTCAATACCTTTGTCGCCCCAAGCAAAACCAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGa                                               <  8:73632/136‑1 (MQ=255)
                                                                                               aaaaaCTCAATACAATTTTAGCCCCAAGAAAAAAAAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCa                                      <  2:139031/135‑1 (MQ=255)
                                                                                                 aaaCTCAATACCATTGTAGCCCCAAGAAAAAACAAACACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCa                                      <  6:106662/134‑1 (MQ=255)
                                                                                                         tACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCAttgctt                            <  5:97395/136‑1 (MQ=255)
                                                                                                          aCCATTGTAGCCCCACGCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCAttgcttg                           <  1:101273/136‑1 (MQ=255)
                                                                                                          aCCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCAttgcttg                           >  3:94497/1‑136 (MQ=255)
                                                                                                                 tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGct                    >  6:178382/1‑136 (MQ=255)
                                                                                                                 tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGct                    >  4:194519/1‑136 (MQ=255)
                                                                                                                 tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGct                    >  7:49604/1‑136 (MQ=255)
                                                                                                                 tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGct                    >  3:193278/1‑136 (MQ=255)
                                                                                                                 tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGct                    >  7:221674/1‑136 (MQ=255)
                                                                                                                 tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGATCATGCGAACGATTTGAATCACGCCCTCTTCACTTAGATATAGTCCAAGCATTGCTTGCACCGct                    >  1:146830/1‑136 (MQ=255)
                                                                                                                  aGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGctc                   >  4:122528/1‑136 (MQ=255)
                                                                                                                  aGCCCCAACCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGct                    <  5:152036/135‑1 (MQ=255)
                                                                                                                    ccccAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGctct                  >  7:202759/1‑135 (MQ=255)
                                                                                                                      ccAAGCAAAAACAAAGAACCGACTCTTACACctct                                                                                                                    <  6:52266/35‑1 (MQ=255)
                                                                                                                      ccAAGCAAAAACAAAGAACCGACTCTTACACctct                                                                                                                    >  5:52266/1‑35 (MQ=255)
                                                                                                                      ccAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTaa               >  7:196570/1‑136 (MQ=255)
                                                                                                                      ccAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTa                >  2:185986/1‑135 (MQ=255)
                                                                                                                      ccAAGCAAAAACAAAGAACAGACTCTTACACCTCTGGCGAGCCCACACCAGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCAAAGCATTGCTTGCACCGCTCTTaa               >  2:201315/1‑136 (MQ=255)
                                                                                                                       cAAGCAAAAACAAAGAACCGACTCTTACACCTCTgg                                                                                                                  >  1:84428/1‑36 (MQ=255)
                                                                                                                        aaGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACt             <  1:158597/136‑1 (MQ=255)
                                                                                                                         aGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                                <  3:81488/100‑1 (MQ=255)
                                                                                                                         aGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                                >  4:81488/1‑100 (MQ=255)
                                                                                                                         aGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACt             >  7:157191/1‑135 (MQ=255)
                                                                                                                           cAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACTaa           >  3:36535/1‑135 (MQ=255)
                                                                                                                           cAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACTaa           >  2:60931/1‑135 (MQ=255)
                                                                                                                            aaaaaCAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACTaaat         >  1:249559/1‑135 (MQ=255)
                                                                                                                            aaaaaCAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACTaaaa         >  3:150200/1‑136 (MQ=255)
                                                                                                                            aaaaaCAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACTaa           >  3:92450/1‑134 (MQ=255)
                                                                                                                            aaaaaCAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACAGCTCTTAACTaaaa         >  7:97730/1‑136 (MQ=255)
                                                                                                                                 cAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACTAAAAGCCCt    <  5:42621/136‑1 (MQ=255)
                                                                                                                                 cAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACTAAAAGCCCt    <  1:24457/136‑1 (MQ=255)
                                                                                                                                   aaGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACTAAAAGCCCTac  <  8:216929/136‑1 (MQ=255)
                                                                                                                                   aaGAACCGACTCTTACACCTCTGGCGAGCACACACCCGCTACGAAGAAATAGATCATGCGAAAGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACTAAAAGCCCTac  <  8:48186/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
CAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACCATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACTAAAAGCCCTAC  >  ACB143/4807027‑4807293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: