| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 434,376 | 0 | G | . | 34.6% | 55.7 / 31.3 | 26 | coding (207/1173 nt) | bioF | 8‑amino‑7‑oxononanoate synthase |
| Reads supporting (aligned to +/- strand): ref base G (7/10); new base . (6/3); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCC > ACB143/434276‑434508 | gcccggggTGGCGGGCCTTCTGCCCCAATCCCAACCTGGGCCGGGCCAACCCCCCCGCGGGGACCGCCGCCGGGCAGGCCGGCGCCGCGCGCGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCcaca < 7:13888/129‑1 (MQ=255) gttgCCGTCTGCCGCAATGACTACCGGGGCCGGGCCAACCACCCCGAGGTGATGGCCGCCGGGCAGGCCGGGGCCGCGCGGTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAc < 8:52129/132‑1 (MQ=255) tGGCTTTATCCACCAATTACAACCGGGGCCTGCCCAACCCCCCCGAGGTGACCCCCGCCTGGCCGGCCGGCGCCCCGGGCGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAcc < 2:53537/135‑1 (MQ=255) gggCTTTCTGCCGCAATGACTACGTGGGCCTGGCCAACCACCCCGCGGGGACGCCCGCCTGCGAGGCGGGCGCCGTGGGGGGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAc < 1:40535/133‑1 (MQ=255) cTTCCGCAGCAATGACTACCGTGGCCTGGCCAACCACCCCCCGGGGATCGCCGCCTGGCCGGCCGGCGCCGAGCGGGGGGGTGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCa < 7:133166/135‑1 (MQ=255) cagcaATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACACCGCCCCATCAGGTTGa > 8:360240/1‑134 (MQ=255) caATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGTTCGGCGCGTGGCTGGCGGGCGCCGTGCGGTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAagag < 1:270056/135‑1 (MQ=255) aCTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACt > 5:335123/1‑136 (MQ=255) aCCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCt > 5:97818/1‑136 (MQ=255) ggCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCGCGCACCTGGTAGTTGGCCACAGCACCCCGCACCCTCAGGTTGAAGAGGCACTGGCTGa > 1:307225/1‑134 (MQ=255) gCCTGGCCAACCACCCCGCGGTGATCGCGGCCTGGCAGGCCGGCGCCGTGCGCTGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGa < 7:212945/133‑1 (MQ=255) cTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCAc > 5:189419/1‑136 (MQ=255) cAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGgcc > 6:317869/1‑136 (MQ=255) aaCCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTTGTTGGCCACAGCACCCCGCACCACCAGGGTAAACAGGCACTGGGTGAACTCACCGgcc > 6:50241/1‑136 (MQ=255) aaCCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCCCCAGCTGGTTGAAGAGGCACTGGCTGACCTCACCGgccg > 5:138662/1‑136 (MQ=255) cccccGAGGTGATCGCCGCCGGGCAGGCCGGCGCCGAGCGCTGGGGTGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGgccgcc < 8:134729/134‑1 (MQ=255) ccccGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCCCCCCGCACCATCAGGTTGAAGAGGCAATGGCTGAACTCACCGGccgccc > 3:248831/1‑135 (MQ=255) ggTGATCCCCGCCGGGCAGGCCGGTGCCTAGCGCTGGGGTGT‑GGGGGGGGGTCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCATGTTGAAGAGGCACTGGCTGAACTCACCGTCCGCCCgcgcgca < 4:27061/135‑2 (MQ=255) cgccgcCTGGCAGGCCGGGGCCGTGGGGTGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCtgtt < 6:182541/135‑1 (MQ=255) gccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACGTGGTAGTTGGCCCCAGCACCCCGCCCCGTCAGGTTGAAGAGGCACTGGCTGACCTCACCGGCCGCCCGCGCGCCCggttg > 7:261856/1‑135 (MQ=255) gccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACCGCACCCCGCACCATCAGGTTGAAGAGGCACGGGATGAACTCACCGGCCGCCCGCGCGCCCggtgg > 2:354915/1‑133 (MQ=255) gccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCGCGCCCCGGGTAGTTGGCCACAGCACCCCGCCCCATCAGGTTGAAGAGGCCCTGGCTGAACTCACCGGCCGCCCCCGCGCCCtgttg > 8:115909/1‑135 (MQ=255) ccTGGCAGGCCGGCGCCGTGCGATGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTtctc < 3:404187/135‑1 (MQ=255) ggCCGGGCGGCGCGGTGCGCGGGGTGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCGCAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCAc < 5:382097/136‑1 (MQ=255) ggCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCGTCGCACGGGGTTGTTGGCCACGGCCCCCCGCCCCGGCGGGTGGATGAGGCCGTGGCTGCCCTCACCGGCCGCCCGCGCGCGCTGTGGTTCTcccc > 3:275729/1‑133 (MQ=255) gggggCGCCGGGGGCGGGGGTGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGc < 8:251369/132‑1 (MQ=255) tgtgGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCCCCAGCAGGTTCAATAGCCACTGGCTGAACGAACCGGCCGCCCGCCCCCCCGGTTGTTCACCACAGGATATATGGCCAATCTGgccgcc > 6:93380/1‑136 (MQ=255) | GCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCC > ACB143/434276‑434508 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |