Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB143 | 3,608,498 | 0 | T | A | 47.8% | ‑6.3 / 10.7 | 23 | intergenic (+21/‑208) | tenA/codB | Aminopyrimidine aminohydrolase/Cytosine permease |
Reads supporting (aligned to +/- strand): ref base T (1/11); new base A (0/11); total (1/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
CTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAAT‑GGGGTTGG‑ACGGGACC‑GTGTAAACC‑GCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGCGAACAGCCC > ACB143/3608364‑3608621 | cTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAAT‑GGGGTTGG‑ACGGGACC‑GTGTAAACC‑GCCGTGGGCTGGGTGAtt < 8:91743/135‑1 (MQ=255) gCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCGGGAGGCGGATTTCTGGCAAAT‑GGGGGTGG‑ACGGGACC‑GTGTAAACC‑GCCGTGGGCTGGGTGATtact < 2:277590/135‑1 (MQ=255) cgcgcAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAAT‑GGGGTTGG‑ACGGGACC‑GTGTAAACC‑GCCGTGAGCTGGGTGATTACTAccca < 5:230207/136‑1 (MQ=255) gTTCGCCTAAGTGGCTGGGTTTTTGGGCCCGGCCTCCCGCGGGGAGGGGGATTGCTGGAAAAG‑GGGGTGGG‑CCGGCACC‑GTGTAACCC‑GCCGGGGGCGGGGTGATAACTACCCCGCCCAGGACCGCTTTGGTGCAt < 6:94782/136‑1 (MQ=255) gCCGAACTGGCAGGTATTTTTGGCCAGGCCGCCCGCCGGGAGGCGGATTTCTGGCAAAT‑GGGGTTGG‑CCGGGACC‑GTGTAACCC‑GCCGGGGGCTGGGTGATAACTACCCAGCCCAGGACCGCTTTGGTGCATggg < 2:344562/135‑1 (MQ=255) cgggAGGCGTATTTCTGGCAATT‑GGGGTGGC‑ACGGCACC‑GTGAAAACC‑GCCGTGGGCGGGGTGATTACTCCCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCg < 7:87222/133‑1 (MQ=255) tgctgATTGCTGGCAATT‑GGGGTTGG‑AAAGGACG‑GTGTAAACC‑GCCGGGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGa < 7:290790/131‑1 (MQ=255) ggCGGATTTGT‑GCAAATGGGGGGGGG‑CCGGCCCC‑GTTAAACCC‑GCGGGGGGGTGTTTGATTACTACCCCCCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGa < 5:241073/135‑1 (MQ=255) ttttCTGTCAATT‑GGGGTTGG‑AGGGGACC‑GTGAAACCC‑GCGGGGGGCGGGGTTATTACAACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCa < 8:234485/134‑1 (MQ=255) aTTTCTGCAAATT‑GGGGGTGG‑CCGGCCCG‑GTGAAACCC‑GCCGGGGGGGGGGTGATTACACCCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCa < 7:43098/135‑1 (MQ=255) tCTGCCAATT‑GGGGTTGG‑CCGGGACC‑GTGTAACCC‑GCCGTGGGCGGGGTGATAACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCt < 6:191788/135‑1 (MQ=255) aatt‑GGGGTTGG‑ACGGGACG‑GTGTAAACC‑GCGGGGGGCGGGTTTATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAAtgt < 5:147098/133‑1 (MQ=255) att‑GGGGTTGG‑CGGGGACC‑TTTAAACCC‑GCCGTGGGTTGGGTTATAAAAACCCCGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAAtgt < 7:18483/133‑1 (MQ=255) att‑GGGGTTGG‑ACGGGACG‑GTGAAAACC‑GCCGTGGGCTGGGTTATAACAACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAAtgt < 2:93414/133‑1 (MQ=255) att‑GGGGTGGC‑CCGGGACC‑GTGTAAACC‑GCCGTGGGCTGGTTTATTACAACCCCGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAAtgt < 3:291247/133‑1 (MQ=255) aatgggggTGGG‑CCGGGACG‑GTGAAACCC‑GGGGTGGGCTGGGTTATAACAACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAAtgt < 5:109511/132‑1 (MQ=255) aaT‑GGGGGTGGCCCGGGACG‑GTTAAAACC‑GCCGTGGGCTGGTTTATAACAACCCCCCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAAtgt < 5:166323/136‑1 (MQ=255) t‑GGGGTGGC‑ACGGCACG‑GTGTAAACC‑GCGGTGGGCTGGGTTATAACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGc < 1:297608/135‑1 (MQ=255) ttgggTTGG‑GCGGGTCC‑GGTTAAACCGGCGGTGGGGTGGGTTATAACAACCCCGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAAtgtg < 3:406705/133‑1 (MQ=255) ggggTTGG‑GAGGGCCCGGTTTAACCC‑GCCGTGGGCGGGGTTATTACACCCCCGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGc < 2:355262/135‑1 (MQ=255) gACG‑GTGTAAACC‑GCCGTGGGGTGGTTGATTACTCCCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATgg < 8:193856/135‑1 (MQ=255) ccc‑gccgGGGGCTGGGTGATAACACCCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGc < 2:75247/134‑1 (MQ=255) ccc‑gccgGGGGCGGGGTGATTACACCCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGc < 2:384705/134‑1 (MQ=255) gccgTGGGCTGGGTGATTACTACCCACCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGTACAGTGCATATGGATTTCTGGGCGaa > 5:269878/1‑135 (MQ=255) ggCGGGGTGAAAACACCCCCGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGCGAACAGccc < 8:208465/135‑1 (MQ=255) | CTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAAT‑GGGGTTGG‑ACGGGACC‑GTGTAAACC‑GCCGTGGGCTGGGTGATTACTACCCAGCCCAGGACCGCTTTGGTGCATGGGCGCCTCAAAATAAGTCGTCCCGCACACTCCAGATCGTCAGATCGCAGCTTTAATGTGCACAGTGCATATGGATTTCTGGGCGAACAGCCC > ACB143/3608364‑3608621 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |