Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,693,1750AC54.8% ‑2.1 / 13.2 44S236R (AGC→CGC) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base A (8/11);  new base C (4/19);  total (12/32)
Fisher's exact test for biased strand distribution p-value = 9.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCG  >  ACB143/5693042‑5693309
                                                                                                                                     |                                                                                                                                      
ggCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGTGCGCCCTTCAGCCCGATAACCAg                                                                                                                                       >  3:308929/1‑135 (MQ=255)
   ccACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAgcgc                                                                                                                                    <  3:330719/135‑1 (MQ=255)
              ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCTTCAGCCAGATCACCACCGCCCGTGgcgcgc                                                                                                                         >  4:47106/1‑135 (MQ=255)
              ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCTTACGCCACCTCACCACCGCCCCGGgcgcgcc                                                                                                                        >  5:124096/1‑135 (MQ=255)
               cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCCCCCGCGCCCGTGGcgcgcg                                                                                                                        >  2:239056/1‑135 (MQ=255)
                aTGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCCTGGCGCGCgg                                                                                                                       >  8:108711/1‑135 (MQ=255)
                 tGGCCATCTTCGTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACGGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGGGTGCGGGCGCAGTTCAGCCAGATCCCCAGCGCCCGTGGCGCGCGGc                                                                                                                      <  2:77010/135‑1 (MQ=255)
                     cAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATc                                                                                                                 <  5:89559/136‑1 (MQ=255)
                       gCTTCCTCGGCCTGCCGGGCATCCCGGCGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCg                                                                                                                <  1:107216/135‑1 (MQ=255)
                        cTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgc                                                                                                               >  2:231522/1‑135 (MQ=255)
                           ccgcgcccTGCGGGGCAGCCCGTCGAGCGCGTAACGCGGGGCCCGGGCGCGAAACCTGCGGTTGGTGGAACTGTGGGGGGTGGGTGCGCACTTCCGCCAGACCCCCCGCGCCGGGGGCGCGCGGCTGATCgcccag                                                                                                           <  6:37405/130‑1 (MQ=255)
                            cTCGGCCTGCCGGGCAGCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGGGCAACCTGCTGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCCGCCATATCACCCGCGCCCGGGGCGCGCGGCTGATCgcccag                                                                                                           <  8:125865/135‑1 (MQ=255)
                             tCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                          <  7:249920/135‑1 (MQ=255)
                               ggCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccaggc                                                                                                         <  5:200459/134‑1 (MQ=255)
                                gcatGCGGGGCATCCCGGATAGGGCCGAAACCGTGGCCGTGGCGCGCAACCGGGGGCTGGGGTAAATGAGCGGCGTGCGTGCGCAGTTCAGCCTGATCCCCCGGGCCGGGGGCGCGCGGCTGATCgcccaggc                                                                                                         <  8:271096/130‑1 (MQ=255)
                                        ggaaTCCCGGACCGCGCCTAAACGGGGGCCCGGGCGCGAAACCTGCTGCTGGTGCACCAGAGCGGCGTGCGTGCGAACTCCCCCCCTACCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                                               <  8:162742/132‑1 (MQ=255)
                                           agcccGGAGAGCGCCGAACCCGGGGCCCTGGCGCGAACCGTGGTGCTGGGGCAACAGCGGGGGGGGCGGGCGCAGTTCCCCCATACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGc                                                                                            <  2:398440/133‑1 (MQ=255)
                                                      cgcCGAACCCGGGGCCCTGGCGCGCACCCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                 <  3:7983/135‑1 (MQ=255)
                                                         cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGGGCACGTGCGCCCGATCACCCGCGCCCCTGGCGCGCGGGGGATCGCCCCCGCCCCCGCCGTGGGCCGGGCGGGTAccgc                                                                              >  3:344042/1‑135 (MQ=255)
                                                          gAAACCGTGGCCCTGGCGCGCAACCTGCTGGTGGTGGAACAGAGCGGGGTGCGTGCGAACTTCCGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                            <  5:366194/136‑1 (MQ=255)
                                                          gAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCACCACCCCCCGTGGCGCGCGGCAGCTCGCCCCGGCCCCCGCCGTGGGCCGGCCGGGAAccgccc                                                                            >  6:66728/1‑135 (MQ=255)
                                                            aacgggggCCCTGGCGCGCACCCTGCTGCTGGTGCAACAGCGCGGCGGGGGTGCGAACTACACCCCGACCACCCGCCCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                            <  5:7829/128‑1 (MQ=255)
                                                             cccGGGGCCCGGGCGCGCACCCTGCTGCTGGGGCAACAGGGGGGGGGGCGGGCGCACTTCACCCAGACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                            <  8:162310/132‑1 (MQ=255)
                                                              ccGGGGCCCTGGCGCGCAACCGGCTGCGGGTGGACCAGCGCGGCGTGCGGGCGCACTCCAGCCAGACCACCCCCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATg                                                                         <  8:239475/135‑1 (MQ=255)
                                                                ggggCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGGGGGGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  7:236518/133‑1 (MQ=255)
                                                                gTGGCCCTGGCGCGCAACCTGCGGCTGGGGGAACAGAGCGGCGTGCGGGCGCACTTCAGCCAGATCACCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  8:204394/135‑1 (MQ=255)
                                                                         ccccgcACCTTGCTGCTGGGGGAAATGAGCGGGGGGGGTGGGCACTTCCGCCCGATCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCCGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGTTGTAGCGCTGTAc                                                              <  6:312070/132‑1 (MQ=255)
                                                                            cgcAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCGGCCGGTAACCGCCCATGTAGCGCTGTaaccg                                                           >  8:386231/1‑131 (MQ=255)
                                                                             gcAACCTGCTGCTGGTGGAACTGAGCGGGGGGCTTGCGCACTTCAGCCAGATCACCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCt                                                         <  5:265281/136‑1 (MQ=255)
                                                                                      tgctgGTGGAACAGCGCGGCGGGCGGGCGCACTTCCGCCCGACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCa                                                 <  7:190425/135‑1 (MQ=255)
                                                                                                     gcggcgGGCGTGCGCACTTCAGCCACATCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                                 <  6:124096/136‑1 (MQ=255)
                                                                                                     gcggcgGGCGGGCGCACTTCACCAAGAGCACCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                                 <  5:130598/136‑1 (MQ=255)
                                                                                                       ggcgTGCGTGCGCACTTGAGCCCGCTCACCCGCGCCCGTGGCGCGCGGCTGTTCGCCCAGGCCCAGGCCGTGGGCCGGGCGGGAAACCCCGCTGTAGAGCTGTTCCAGCTTTTCCGCACGGAAGAGgcgcggggc                                >  2:266720/1‑130 (MQ=255)
                                                                                                       ggcgTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                                <  7:219061/135‑1 (MQ=255)
                                                                                                       ggcgTGCGTGCGAACTTCAGCATGATAACCGGCGCCCGTGGCCCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                                <  7:7319/135‑1 (MQ=255)
                                                                                                          ttgcgtgcgATCTTCCGCCAGATCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCTCCGATGTAGAGCTTTACTATCTTATTCTCACGGACGAGTCGCTGCGCTAGt                            <  6:134663/135‑1 (MQ=255)
                                                                                                           tgcgtgcgCACTTCAGCCAGATCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                            <  8:188490/135‑1 (MQ=255)
                                                                                                           ggcgtgcgCACTTCAGCCAGATCACCATCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                            <  2:375292/134‑1 (MQ=255)
                                                                                                            gggtgcgCACTTCCCCCTCATCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt                           <  1:396341/133‑1 (MQ=255)
                                                                                                            gcgggcgCACTTCACCCCCCTCCCCCGCGCCCGGGCCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt                           <  3:358269/135‑1 (MQ=255)
                                                                                                                gcgcACTTCAGCAACATCCCCGCCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                       <  2:400678/135‑1 (MQ=255)
                                                                                                                      ttCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCt                  >  3:44058/1‑134 (MQ=255)
                                                                                                                             ccaTCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACg          <  3:235735/133‑1 (MQ=255)
                                                                                                                                   ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgc    >  1:273695/1‑135 (MQ=255)
                                                                                                                                     aGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgccg  <  8:391108/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
GGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCG  >  ACB143/5693042‑5693309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑