Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	2,619,534	C→A	Q37K (CAA→AAA)	CJ019_02478 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	2,619,534	0	C	A	79.3%	121.3 / 28.8	58	Q37K (CAA→AAA)	CJ019_02478	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (7/5);  new base A (23/23);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 7.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGGCAAAGGACACCACGATGATTGCAAAAGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGCAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCGGAGCAGCTGCAGGCCATGCGCCTGGAAACGCACCGTGAAGTGGCC  >  ACB143/2619407‑2619665                                                                                                                                |                                                                                                                                    gCCGGCAAAGGACACCACGATGATTGCAAAAGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCg                                                                                                                               <  8:6942/134‑1 (MQ=255) gCCGGCAAAGGACACCACGATGAATGCAAAACAATCCCGTCAGCCCCTCGCGCTGCAGCGCTTTCCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCCCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAg                                                                                                                             >  6:86861/1‑136 (MQ=255)     gCAAAGGACAGCACGCTGATTGCAAAAGACGCCCGTCAGGCGCTGGCGCTGCAGCGCTTTGCCGAAGCCTATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTgg                                                                                                                          <  3:281843/135‑1 (MQ=255)     gCAAAGGACACCACGATGATTGCAAAAGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTgg                                                                                                                          <  7:200552/135‑1 (MQ=255)         aGGACACCACGATGATTGCAAAAGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGt                                                                                                                      >  4:277222/1‑135 (MQ=255)             caccacGATGATTGCAAAAGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGCAAATCGAGTGGGCGTTCGa                                                                                                                  <  7:143483/135‑1 (MQ=255)                        ttGCAAAAGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGCAAATCGAGTGGGCGTTCGAGGATgccgcc                                                                                                        >  6:131718/1‑134 (MQ=255)                          gCAAAAGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGCAAATCGAGTGGGCGTTCGAGGATGCCGCCGAg                                                                                                     >  6:66177/1‑135 (MQ=255)                          gCAAAAGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAg                                                                                                     <  6:275752/135‑1 (MQ=255)                           cAAAAGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAg                                                                                                     >  3:189875/1‑134 (MQ=255)                            aaaaGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAgg                                                                                                    <  4:180818/134‑1 (MQ=255)                            aaaaGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGa                                                                                                   <  3:141459/135‑1 (MQ=255)                            aaaaGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGa                                                                                                   <  2:156769/135‑1 (MQ=255)                            aaaaGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGa                                                                                                   <  4:142988/135‑1 (MQ=255)                            aaaaGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGa                                                                                                   <  3:29763/135‑1 (MQ=255)                            aaaaGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGa                                                                                                   <  3:43333/135‑1 (MQ=255)                            aaaaGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGa                                                                                                   <  7:116128/135‑1 (MQ=255)                             aaaGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGa                                                                                                   >  7:66558/1‑134 (MQ=255)                             aaaGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGa                                                                                                   >  7:218998/1‑134 (MQ=255)                                  agCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAggg                                                                                             <  3:7928/135‑1 (MQ=255)                                  agCCCGGCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAggg                                                                                             <  1:84102/135‑1 (MQ=255)                                   gCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTTTGCTTTAGATGATGCAGCCGAGGAACAGGGt                                                                                            <  1:183486/135‑1 (MQ=255)                                    cccGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGCAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGACCAGGGTa                                                                                           >  2:368481/1‑135 (MQ=255)                                    cccGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGCAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTAt                                                                                          >  6:99965/1‑136 (MQ=255)                                    cccGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTa                                                                                           <  1:397688/135‑1 (MQ=255)                                    cccGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTa                                                                                           <  8:176807/135‑1 (MQ=255)                                    cccGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCGGGATTCGCGTGAGCAGGCCCAGCAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGTGTa                                                                                           <  4:137748/135‑1 (MQ=255)                                     ccGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTAt                                                                                          <  8:20317/135‑1 (MQ=255)                                                    gcTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCt                                                                           >  4:109735/1‑135 (MQ=255)                                                    gcTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCt                                                                           >  3:136744/1‑135 (MQ=255)                                                         aGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGgcgc                                                                      >  1:341281/1‑135 (MQ=255)                                                           cgcTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGACCAGGGTATTGAGCCTTGGGAGCTGGCGCTg                                                                    >  6:240249/1‑135 (MQ=255)                                                           cgcTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTg                                                                    >  5:399268/1‑135 (MQ=255)                                                           cgcTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTg                                                                    >  6:296195/1‑135 (MQ=255)                                                                 gCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAat                                                                <  3:179246/133‑1 (MQ=255)                                                                   cGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAattgatt                                                           >  6:143349/1‑136 (MQ=255)                                                                   cGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAattgatt                                                           >  6:376272/1‑136 (MQ=255)                                                                   cGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAattgat                                                            >  7:79701/1‑135 (MQ=255)                                                                      aGCCAATCCGGAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGTCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCTAGGAAGAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGc                                                         <  4:229825/135‑1 (MQ=255)                                                                          aaTCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGaa                                                     >  7:24048/1‑135 (MQ=255)                                                                             ccGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGCAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATccc                                                 >  5:315567/1‑136 (MQ=255)                                                                               gCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGCAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCg                                               <  5:131718/136‑1 (MQ=255)                                                                               gCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGCAAATCGAGTGGGCGTTCGAGGAGGCCGCCGATGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCg                                               <  5:211213/136‑1 (MQ=255)                                                                               gCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATcccc                                                >  2:276992/1‑135 (MQ=255)                                                                               gCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATcccc                                                >  4:112760/1‑135 (MQ=255)                                                                               gCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCg                                               >  6:132014/1‑136 (MQ=255)                                                                                cAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGCAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCg                                               <  2:190630/135‑1 (MQ=255)                                                                                    tGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCGGa                                             >  3:291133/1‑133 (MQ=255)                                                                                       tCGATGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCGGagcag                                         <  4:336153/134‑1 (MQ=255)                                                                                              gaTCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCGGAGCAGCTGCAGGcc                                <  6:361567/136‑1 (MQ=255)                                                                                                   gcgAGCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCGGAGCAGCTGCGGGCCATgc                            <  7:31578/135‑1 (MQ=255)                                                                                                       gCTGGATGCGCGTGAGCAGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCGGAGCAGCTGCAGGCCATGCGCCt                        >  7:174200/1‑135 (MQ=255)                                                                                                           gATGCGCGTGAGCAGGCCCAGAAAATCGAGGGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCGGAGCAGCTGCAGGCCATGCGCCTGGaa                    <  3:180008/135‑1 (MQ=255)                                                                                                                    gAGCAGGCCCAGCAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCGGAGCAGCTGCAGGCCATGCGCCTGGAAACGCACCg            >  3:95012/1‑134 (MQ=255)                                                                                                                        aGGCCCAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCGGAGCAGCTGCAGGCCTTGCGCCTGGAAACGCACCGTGAAg       >  2:94397/1‑135 (MQ=255)                                                                                                                             cAGCAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCGGAGCAGCTGCAGGCCATGCGCCTGGAAACGCACCGTGAAGTGGcc  >  5:131578/1‑135 (MQ=255)                                                                                                                             cAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCGGAGCAGCTGCAGGCCATGCGCCTGGAAACGCACCGTGAAGTGGcc  >  6:293274/1‑135 (MQ=255)                                                                                                                             cAGAAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCGGAGCAGCTGCAGGCCATGCGCCTGGAAACGCACCGTGAAGTGGc   >  2:57618/1‑134 (MQ=255)                                                                                                                                |                                                                                                                                    GCCGGCAAAGGACACCACGATGATTGCAAAAGAAGCCCGTCAGGCCCTGGCGCTGCAGCGCTTTGCCGAAGCCAATCCGCAGTTGCTCGAGGAGATCCGCGAGCTGGATGCGCGTGAGCAGGCCCAGCAAATCGAGTGGGCGTTCGAGGATGCCGCCGAGGAACAGGGTATTGAGCCTTGGGAGCTGGCGCTGCAATTGATTGCCGAATCCCCGGAGCAGCTGCAGGCCATGCGCCTGGAAACGCACCGTGAAGTGGCC  >  ACB143/2619407‑2619665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑