Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,175,5520AC46.0% 21.0 / 13.8 37N49T (AAC→ACC) tadAtRNA‑specific adenosine deaminase
Reads supporting (aligned to +/- strand):  ref base A (13/7);  new base C (0/17);  total (13/24)
Fisher's exact test for biased strand distribution p-value = 2.24e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCATCGATCGCAGTCGCGATCAGGAATTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGT‑GCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAACCGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCATGT  >  ACB143/1175420‑1175683
                                                                                                                                     |                                                                                                                                    
tcatcGATCGCAGTCGCGATCAGGAAATCAAGCGCCTGGCGCTGGGCTTGGCCGCCGAAGGCGCGGCGGTGGGCGAGGTGCCAGTGGGGGCGGT‑GCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAACCg                                                                                                                                   >  8:3234/1‑136 (MQ=255)
      atcgCAGTCGCGATCAGGAATTAATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGT‑GCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAACCGGCCG‑at                                                                                                                            >  3:68299/1‑136 (MQ=255)
                                        gcTGGCCTTGGCCGCCGAAGGCGCGGCGTTGCGCGAGGTGCCAGTGGGGGCGGT‑GCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGGTTCAACCGTCCG‑ATCATCGACAGCGCCCCCAGCGCGCATGCCGAGAtg                                                                                          >  5:23111/1‑136 (MQ=255)
                                               ttGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGT‑GCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAACCGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGa                                                                                   >  4:46939/1‑136 (MQ=255)
                                                    cgccgAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGT‑GCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAACCGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAgg                                                                              >  1:235793/1‑136 (MQ=255)
                                                    cgccgAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGT‑GCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAACCGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAgg                                                                              >  5:65186/1‑136 (MQ=255)
                                                                     ggggCGGGGTGCCTGGGGGGGCGGG‑GGGGGGGCCGCTGGGGCCGGTTCCCGCCCTGGGTCCCACCCGGCCT‑AACATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGc                                                             <  8:302201/135‑1 (MQ=255)
                                                                               gCCAGTGGGGGCGGT‑GCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAACCGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTAc                                                   >  2:125253/1‑136 (MQ=255)
                                                                                cctgggggggCGGG‑GCGGGTGCCGCTGGGGCTGGTGACCGGCCTGGGTTACACCCGGCCG‑AGCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTAcc                                                  <  4:232753/131‑1 (MQ=255)
                                                                                  cgggggggCGGG‑GGGGGGCCAGCATGGGCAGGTTAGCGGCCTGGGTTTCACCCGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCgg                                                <  4:242102/133‑1 (MQ=255)
                                                                                  cgggggggCGGG‑GCGGGTGCCCCAGGGGCCGGTCCTCGGCCCGGGTGTCACCCGGCCT‑AACATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCgg                                                <  6:53359/133‑1 (MQ=255)
                                                                                    tGGGGGCGGT‑GCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAACCGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGc                                               >  8:97150/1‑135 (MQ=255)
                                                                                     ggggggggTGGCGGGGGCAGCTGGGGCTGGGCACCGGCCAGGGTTCCACCCGCCCG‑AACATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCt                                              <  7:35069/136‑1 (MQ=255)
                                                                                         gCGGT‑GCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAACCGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCg                                         >  5:244250/1‑136 (MQ=255)
                                                                                            gt‑ggtgGGGCCGCAGGGGCAGGTGATGGGCCAGGGTTTCACCGGGCCT‑ACCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGgca                                      <  3:6157/136‑1 (MQ=255)
                                                                                               gctggggctgctggggctGGTGAGCGGCCCGGGTTTCACCCGGCGG‑AAAATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGgcagc                                    <  1:21264/136‑1 (MQ=255)
                                                                                                       tgcaTGGGCAGGTGATCGGCCAGGGTTTAACCCGGCCT‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                            <  7:89471/135‑1 (MQ=255)
                                                                                                       agctggggCTGGTGAGCGGCCTGGGTTTCACCGGGCGT‑ATAATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                            <  2:235793/131‑1 (MQ=255)
                                                                                                       agctTGGGCTGGTTATCGGCCTGGGTTTCAACCGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                            <  8:240782/136‑1 (MQ=255)
                                                                                                       agctTGGGCTGGGGCGCGCCCTGGGTTTCAACGGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                            <  2:237227/136‑1 (MQ=255)
                                                                                                       agcaTGGGATGGGCAGCGGCCTGGGTTTCAACCGGCCT‑ATAATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                            <  6:288112/136‑1 (MQ=255)
                                                                                                       agattGGGCTGGTGATCGGCCTGGGTTTCAACGGGCCT‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                            <  4:50536/132‑1 (MQ=255)
                                                                                                         ctggggCTGGGGAGCGGCCGGGGTTCACCCCCGCCA‑AACCTCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                            <  6:207328/131‑1 (MQ=255)
                                                                                                           tGGGCAGGTGATCGGCCAGGGTTTCAACCGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTAtgt                         >  3:24579/1‑135 (MQ=255)
                                                                                                                  gtgttgggCTAGGGTTTCACCGGGCGT‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTg                  <  3:124977/129‑1 (MQ=255)
                                                                                                                  gtgtTCGCCCCGGGTTACACCCGGCCG‑TTCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTgg                 <  2:257852/136‑1 (MQ=255)
                                                                                                                  gggagcGGCCGGGTTTCCACCCGCCCT‑ATCCTCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTgg                 <  3:45896/131‑1 (MQ=255)
                                                                                                                    tagcGGCCAGGTTTTCACCCGGCCT‑ATAATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGaa               <  4:145772/133‑1 (MQ=255)
                                                                                                                      tCGGCCAGGGTTTCAACCGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAAcc             >  5:17368/1‑136 (MQ=255)
                                                                                                                          ccAGGGTTTCAACCGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCTGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCCGCCCCCTGTATGTGACGCTGGAACCGtt          >  7:307044/1‑134 (MQ=255)
                                                                                                                            tgggTTT‑AAACGGCCGTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTgcag       <  8:3647/135‑1 (MQ=255)
                                                                                                                              tgTTCCACCCGCCCT‑ATCATCGACAGCGACCCCAGCGCGCATGCAGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTgcag       <  2:216977/133‑1 (MQ=255)
                                                                                                                              ggTTCCACCCGGCCT‑ATAATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTgcag       <  4:20334/134‑1 (MQ=255)
                                                                                                                              ggTTACAACCGCCCT‑ATAATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTgcag       <  3:262462/134‑1 (MQ=255)
                                                                                                                               gTTTCAACCGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCAt    >  8:235837/1‑136 (MQ=255)
                                                                                                                                  tCACCCGCCCT‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCAtgt  <  2:213200/135‑1 (MQ=255)
                                                                                                                                  ccAACGGGCCT‑ACAATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCAtgt  <  7:199358/134‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
TCATCGATCGCAGTCGCGATCAGGAATTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGT‑GCTGGTGCAGCATGGGCAGGTGATCGGCCAGGGTTTCAACCGGCCG‑ATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCATGT  >  ACB143/1175420‑1175683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑