| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 1,277,828 | 0 | T | G | 55.6% | 1.2 / 10.5 | 19 | intergenic (+20/‑70) | pal_2/ompA_1 | Peptidoglycan‑associated lipoprotein/Outer membrane protein A |
| Reads supporting (aligned to +/- strand): ref base T (3/5); new base G (6/4); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.08e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCAGCAGCTTCGTTTCGGTGGTCGGCGCGGGTGAAACCCAGCCGGTGGCAGACAACGCCACGGCCGAAGGGCGTGCCATGAACCGTCGTACCGAGATCAAGATCCAGCGGTAAGACGGCAGGCGCCCGTGCCTCGAGCAGTGGCACGGGCGCGTCTTCATGCCGGCCAGCCGTTTGTGGCCAATGACACAGGAGCATTCAGCATGAGTGTGACGTCGAAGGCGGCTTTGCCGCTGTTGGTGGCTGCCAGCCTGCTCACGGGCT > ACB143/1277694‑1277958 | gcgcAGCTGCTTCGTTTCGGGGGTCGGCGCGGGTGAACCCCAGCCGGTGGCAGACAACGCCACGGCCGAAGGGCGTGCCATGAACCGTCGTACCGAGATCAAGATCCAGCGGTAAGACGGCAGGCGCCCGTGCCTc < 6:22503/136‑1 (MQ=255) cagcagCTTCGTTGCGGGGGTCGGCGCGGGTGAAACCCAGCCGGTGGCCGACAACGCCACGGCCGAAGGGCGTGCCATGAACCGTCGTACCGAGATCAAGATCCAGCGGTAAGACGGCAGGCGCCCGTGCCTCGAg < 5:20338/136‑1 (MQ=255) agcagcTTCGTTTCGGTGGTCGGCGCGGGTGAAACCCAGCCGGTGGCAGACAACGCCACGGCCGAAGGGCGTGCCATGAACCGTCGTACCGAGATCAAGATCCAGCGGTAAGACGGCAGGCGCCCGGGCCGCGGGc > 1:86065/1‑136 (MQ=255) agcagcTTCGTTTCGGTGGTCGGCGCGGGTGAAACACAGCCGGTGGCAGACAACGCCACGGCCGAAGGGCGTGCCATGAACCGTCGTACCGAGATCAAGATCCAGCGGTAAGACGGCAGGCGCCCGTGCCGCGGGc > 7:52958/1‑136 (MQ=255) gtCGGCGCGGGTGAAACCCAGCCGGTGGCAGACAACGCCACGGCCGAAGGGCGTGCCATGAACCGTCGTACCGAGATCAAGATCCAGCGGTAAGACGGCAGGCGCCCGGGCCGCCCGCAGTGGCCCGGGcgcgggt > 8:268596/1‑133 (MQ=255) gtCGGCGCGGGTGAAACCCAGCCGGTGACAGACAACGCCACGGCCGAAGGGCGTGCCATGAACCGTCGTACCGAGATCAAGATCCAGCGGTAAGACGGCAGGCGCCCCTGCCGCGAGCACTGGGCCCGGcgcgggg > 3:223873/1‑133 (MQ=255) cAGCCGGTGGCAGACAACGCCACAGCCGAAGGGCGTGCCATGAACCGTCGTACCGAGATCAAGATCCAGCGGTAAGACGGCAGGCGCCCGTGCCGCCAGCAGTGGCACGGGCGCGTCTTCATGCCGGCCCGCCGtt > 8:256527/1‑136 (MQ=255) aGACAACGCCACGGCCTAAGGGCGTGCCATTAACCGTCGTACCGACATCAAGATCCAGCGGAAAGACGGCAGGCGCCCGTGCCTCGAGCAGTGCCACGGGCGCGTCTTCATGCCGGCCAGCCGTTTGTGGCCAATg < 7:240754/136‑1 (MQ=255) cATGAACCGTCGTACCGAGATCAAGATCCAGCGGTAAGACGGCAGGCGCCTGTGCCTCGAGCAGTGGCACGGGCGCGTCTTAAAGCCGGCCAGCCGTTTGTGGCCAATGACCCAGGAGCATTCAGCATGAGTGTGa > 2:132026/1‑136 (MQ=255) cATGAACCGTCGTACCGAGATCAAGATCCAGCGGTAAGACGGCAGGCGCCCGTGCCGCGCGCACTGGCCCGGGCGCCGGGTAAAGCCCGCCCCCCGGTTGTGGGCAATTACACACGACCCTAAAACATGACtgtg > 8:260722/1‑135 (MQ=255) cATGAACCGTCGTACCGAGATCAAGATCCAGCGGTAAGACGGCAGGCGCCCCTGCCTCGAGCAGTGGCCCGGGGGCGGCGTGATGGCGGCCCGCCGGTTGTGGCCCAAGACACAGGAGCATTAAGCATGAGggtgg > 6:105157/1‑135 (MQ=255) tACCGAGATCAAGATCCAGCGGTAAGACGGCAGGCGCCCCTGCCACGCGCAGTGGCACGGGCGCGTCGTGATTCCCGCCCCCCGGTTGTGGGCAATGAACCAGGCGCATTAAGCCTGCGTGGGACGGGGAAggcgg > 8:169275/1‑136 (MQ=255) aaGATCCAGCGGTAAGACGGCAGGCGCCCGTGCCTCGCGCAGTGGCACGGGCGCGTCTTCAAGCCGGCCAGCCGGTTGTGGCCAATTACACAGGAGCATTCAGCATGAGTGTGACGTCGAAGGCGGCGTTGCCGcg > 7:74519/1‑135 (MQ=255) aaGCCGGCCGGCGCCCGTGCCTCGCCCCGTGGCACGGGCGCGTCTTCATGCCGGCCAGCCGTTTGTGGCCAATGACACAGGAGCATTCAGCATGAGTGTGACGTCGAAGGCGGCTTTGCCGCTGTTGGTGGCTGcc < 3:276772/136‑1 (MQ=255) ccccGGCTGGCGCCCGTGCCGCGAGCAGGGGCAGGGGCGCGTCTTCATGCCGGCCAGCCGTTTGTGGCCAATGACACAGGAGCATTCAGCATGAGTGTGACGTCGAAGGCGGCTTTGCCGCTGTTGGTGGCTGCCa < 1:290056/133‑1 (MQ=255) aGACGGCAGGCGCCCGGGCCTCGAGCAGTGGCACGGGCGCGTCTTCATGCCGGCCAGCCGTTTGTGGCCAATGACACAGGAGCATTCAGCATGAGTGTGACGTCGAAGGCGGCTTTGCCGCTGTTGGTGGCTGCCa < 7:236186/136‑1 (MQ=255) gccggCGCCGGTGCCGCGCGCTGTGGCCCGGGCGCGTCTTCATGCCGGCCAGCCGTTTGTGGCCAATGACACAGGAGCATTCAGCATGAGTGTGACGTCGAAGGCGGCTTTGCCGCTGTTGGTGGCTGCCAGCCTg < 6:125820/133‑1 (MQ=255) cccGTGCCGCGAGCCGGGGCACGGGCGCGTCTTCATGCCGGCCAGCCGTTTGTGGCCAATGACACAGGAGCATTCAGCATGAGTGTGACGTCGAAGGCGGCTTTGCCGCTGTTGGTGGCTGCCAGCCTGCTCACgg < 4:223873/136‑1 (MQ=255) gggCCGCGAGCTGGGGCAGGGGCGCGTCTTCATGCCGGCCAGCCGTTTGTGGCCAATGACACAGGAGCATTCAGCATGAGTGTGACGTCGAAGGCGGCTTTGCCGCTGTTGGTGGCTGCCAGCCTGCTCACGGGCt < 5:315930/134‑1 (MQ=255) | GCGCAGCAGCTTCGTTTCGGTGGTCGGCGCGGGTGAAACCCAGCCGGTGGCAGACAACGCCACGGCCGAAGGGCGTGCCATGAACCGTCGTACCGAGATCAAGATCCAGCGGTAAGACGGCAGGCGCCCGTGCCTCGAGCAGTGGCACGGGCGCGTCTTCATGCCGGCCAGCCGTTTGTGGCCAATGACACAGGAGCATTCAGCATGAGTGTGACGTCGAAGGCGGCTTTGCCGCTGTTGGTGGCTGCCAGCCTGCTCACGGGCT > ACB143/1277694‑1277958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |