Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,094,8080AC51.2% ‑4.0 / 14.5 43L186R (CTG→CGG) CJ019_01995hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (14/7);  new base C (12/10);  total (26/17)
Fisher's exact test for biased strand distribution p-value = 5.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAG  >  ACB143/2094683‑2094936
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gggCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCCCGccgg                                                                                                                         >  5:624/1‑134 (MQ=255)
    cATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCg                                                                                                                     >  5:109816/1‑136 (MQ=255)
       gCGGGCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCACAAGATACGTCAGCGGCTCTTCATCCCCATTCGGCTCCGGCTGCTTGCGAAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGc                                                                                                                  <  4:280976/136‑1 (MQ=255)
                   cTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACAGCACGCCGCGCTCGAAGGCCACGCGCGCCTGGCCGGGCTGGCC‑AGTTTgg                                                                                                     >  5:113693/1‑135 (MQ=255)
                        ctttGGCCGGGCCCAACAGTTCCTCACTATACGGCCGCGGCTCTCAAAACACCTGCGGCGCCGGCGGCTGCCCAAAATCCTCCCCGCCGCGCGCGACGGCCCGGCGCGCCGGGCCGGGCTGGCC‑TTTTTGCAGGGc                                                                                                <  2:192188/134‑1 (MQ=255)
                        cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGGCCGGGCTGGCC‑GTTTTGCAGGGc                                                                                                >  1:275913/1‑136 (MQ=255)
                        cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGGCCGGGCGGGCC‑GGTTTGCAGGGc                                                                                                >  1:100970/1‑136 (MQ=255)
                        cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCATGCGCGCCTGGCCGGGCTGGCC‑GTTTTGCAGGGc                                                                                                >  6:258846/1‑136 (MQ=255)
                        cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCCCCTGGCCGGGCGGGCC‑TTTTTGCAGGGc                                                                                                >  1:117272/1‑136 (MQ=255)
                        cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACATTCGGCTCCGCCTGCTTGCGCAAATACTTCACGCCTCGCTCCAACGCCATGCGCGCCTGGCCCGGGTGGCC‑GTTTTGCCGGGc                                                                                                >  8:168980/1‑136 (MQ=255)
                             gCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGGCCGGCGCGGCGCGCCGGGCGGGCCGGTTTTGCTGGGcgggt                                                                                            >  3:174072/1‑132 (MQ=255)
                             gCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATAATGCACGCCGCGCTCGAAGGCCCGGCCCCCCGGGGCGGGGTGGGG‑GTTTTGGAggggcggtg                                                                                           >  3:316801/1‑130 (MQ=255)
                                     cAACATTTTCTCCCTATACGTCGGATGCTCTTAATACCCCTTCGGCTCCGTCTGCTTCCTAAAAAACTCACCCCCGCGCTCGACGGCCAGGCGCGCCGGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAgg                                                                                   <  6:66487/136‑1 (MQ=255)
                                     aaaccttttCTAAATCTACGGCCGCTGCTCTTCAACCACCTTCGGCTCCGGCGGCTTGCGAAAATCCGCCACGCCGGGGTCTACGGCCAGGCGCGCCGGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAgg                                                                                   <  2:310111/130‑1 (MQ=255)
                                     aaaCTGTTTCTAAATAACCGTCCGCGGCTCTTAAAACACCTTCGGCGCGGGGGGCTTGCCAAAATACTCCACGCCGCGCTCGACGGCCCGGCGCGCCTGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAgg                                                                                   <  2:129220/135‑1 (MQ=255)
                                          gTTTCTCAAGATCCGTCAGCTGCTCTACATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTCCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGgttgtt                                                                              <  2:222636/136‑1 (MQ=255)
                                          gTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCGGGCC‑GTTTTTCAGGGCCTGTTGGGCGAGgttgtt                                                                              >  1:129220/1‑136 (MQ=255)
                                           tttCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGTGCCTGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGgttgtt                                                                              <  1:28914/135‑1 (MQ=255)
                                             catcAATATAGGTCGTCTCCTCTCAATACACCTTCGCTTCGGGCTGCTTGCGCAAAAACTCCTCGCCGCGCTCTAAGGCCCGGGGCGCCTGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTga                                                                            <  2:264781/133‑1 (MQ=255)
                                                cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCTGGCCGGGCTGGCG‑GTTTTTCAGGGGCGGTTGGGGGAGGGTGTTggaga                                                                         >  7:76349/1‑135 (MQ=255)
                                                     tccGTCAGCTGCTCTTCATCCCCCTGCGGCGCGGGCTGCTTGCCAAAAAACTCCCCGCCGCGCTCGACGGCCCGGCGCGCCGGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAActct                                                                    <  4:174072/133‑1 (MQ=255)
                                                        gTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGTGCCGGGCTGGCC‑TTTTTTAAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTatat                                                                >  6:304502/1‑136 (MQ=255)
                                                          cAGCTGCTCTTCATACCCCTTCGGCTCCGGCGGCTTGCGAAAAAACACCACGCGGCGCGCGACGGCCCGGCGCGCCGGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgc                                                              <  5:258064/136‑1 (MQ=255)
                                                                ctctTCCTACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCGCCTGGCCGCGCGGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcagcag                                                         >  4:224889/1‑135 (MQ=255)
                                                                atattCATCCCCCTTGGGCTCCGGCTGCTTCCGCAAATTCCGCCCGCCGCGCTCGACGGCCCGGCGCGCGGGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcagcag                                                         <  8:201298/132‑1 (MQ=255)
                                                                   ttCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCCCCAGGCCGGGCTGGCC‑GTTTTTCATGGCCGGTTGGCCGCGGTTGTTGTATAACTCTATATTCAGCCTCAcc                                                     >  6:190240/1‑136 (MQ=255)
                                                                     cATACCCGTTCGGCCGCGGATGCTTGCTAAAATCCTGCACGCCGCGCTCGACGGCCCGGCGCGCCGGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCa                                                    <  4:177312/135‑1 (MQ=255)
                                                                     cATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCTGGCCGGGCGGGGC‑GTTTTTCAGGGCCTGTTGGGCGAGGGTGTTGTAGAACTCTATAATCAGCAGCAcccc                                                   >  4:47492/1‑134 (MQ=255)
                                                                         cacCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGGCCCGGGTGGCC‑GTTTTGCAGGGCCTGTGTGCCGACGTTGTTGTAGAACTATATATGCAGCACCACCAGGat                                                >  5:250809/1‑135 (MQ=255)
                                                                             ttCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCGGGCC‑GTTTTGGAGGGGCGGTTGGCCGAGGTTGTTGAAGAACTCGATATGCAGCCGCACCAGGATATGGc                                           >  5:330211/1‑136 (MQ=255)
                                                                                ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCTGGCC‑TTTTTGCAGGGCCGGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGa                                        >  6:96016/1‑136 (MQ=255)
                                                                                ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGa                                        >  8:232371/1‑136 (MQ=255)
                                                                                ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCC‑GTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGAAGCAGCACCAGGATATGGCGGa                                        >  3:252040/1‑136 (MQ=255)
                                                                                 gCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCGGGCC‑TTTTTGCTGGGCCGGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAt                                       >  7:36377/1‑136 (MQ=255)
                                                                                  cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGGCGGGCTGGCC‑GTTTTGGAGGGCCGGTTGGGCGAGGTTGTTGTAGAACTCTATATTGAGCGGCACCAGGGTATGGCGGGtt                                      >  1:182240/1‑136 (MQ=255)
                                                                                  cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGGC‑TTTTTGCAGGGCCTGTTGGCCGAGGGTGTTGTAGCACTCTATATGCAGCAGCACCAGGATATGGCGGAtt                                      >  1:281587/1‑136 (MQ=255)
                                                                                              gcgaAAATACTGCCCGCCGCGCGCGACGGCCAGGCGCGCCTGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCaa                          <  2:220480/136‑1 (MQ=255)
                                                                                               cgcAAATACTGCACGCCGCGCTCGACGGCCAGGCGCGCCTGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCaa                          <  2:100970/135‑1 (MQ=255)
                                                                                                            cgccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTTGCCTCGa             >  6:79587/1‑135 (MQ=255)
                                                                                                                   cgcgccggCCCGGCGCGCGTGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGc     <  7:140095/130‑1 (MQ=255)
                                                                                                                      gAAGGCCCGGCGCGCCGGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  3:320600/136‑1 (MQ=255)
                                                                                                                          gCCCGGCGCGCCTGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  1:207383/132‑1 (MQ=255)
                                                                                                                          gCCAGGCGCGCCTGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  >  2:207383/1‑132 (MQ=255)
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GGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCC‑TTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAG  >  ACB143/2094683‑2094936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑