Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,730,1490TG41.5% 27.3 / 13.2 41V1336G (GTG→GGG) CJ019_02576hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (12/12);  new base G (0/17);  total (12/29)
Fisher's exact test for biased strand distribution p-value = 3.81e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCTTGAGTAACCAGGACCTTCTC  >  ACB143/2730025‑2730282
                                                                                                                            |                                                                                                                                     
gTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGggcgggcg                                                                                                                            >  5:287738/1‑136 (MQ=255)
      atatGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAat                                                                                                                      >  5:332561/1‑136 (MQ=255)
                 gAGGGACAGCGGATCTGCCCGGCCTGAGCGTGCCTTGCTGAAGCTGGGTGCCCGGATGTGGGGGTTGGGGGTGCTTGAGATAATTGCCGCTGAAAATGCTTATCGGTTGGGGGCGGGCGCTGAATCGATCGAATTc                                                                                                           <  5:87352/136‑1 (MQ=255)
                 gAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTc                                                                                                           >  5:238822/1‑136 (MQ=255)
                 gAGCCACAGCGGCTCGGCCGGGCCTGCGGGGGCGTTGGTGACGCGGGGGGCCGGGAGGGGGGGGTTGGGGGTGCTTGAGAAAATTGCCGCTTAACATGCTTTTGGGGGGGGGGGGGGCGCTGAATCGATCGAATTc                                                                                                           <  2:7150/136‑1 (MQ=255)
                      acaGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCtt                                                                                                      >  2:164193/1‑136 (MQ=255)
                             aTCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTAt                                                                                               >  5:273279/1‑136 (MQ=255)
                              gctccccGGCCTGCGCGGGCCTTGCTTACGCTCGGTGCCCGGATGGGGCGGTGGGCGGTGCTTGATAAACTTGCCGCTGAACATGCTTAGCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATc                                                                                              <  8:46456/132‑1 (MQ=255)
                                tGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCg                                                                                             >  2:162889/1‑135 (MQ=255)
                                     ggCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCgg                                                                                       >  3:197671/1‑136 (MQ=255)
                                          gAGCGGGCCTTGCTGACGCTCGGGGCCCGGATGTGGCGGTTGGGGGTGCTTGAGAAATTTGCCGCTGAACATGCTTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTgc                                                                                  <  4:263987/136‑1 (MQ=255)
                                                     ggtGATGCGCGGGGCCGGGTTGGGGGGGTTGGCGTTGCTTGAGAAAATTGCCGCTGAACATGGTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTctct                                                                        <  3:293208/133‑1 (MQ=255)
                                                     ggtGACGCTCGGGGCCCGGTTGTGGGGGTTGGCGGGGCTTGAGAAATTTGCGGCGGAAATTGCTATTGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGAAAGTctct                                                                        <  1:66968/133‑1 (MQ=255)
                                                              cgggccccGGATGGGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCt                                                              <  5:217684/131‑1 (MQ=255)
                                                               ggTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCGTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTg                                                             >  8:229907/1‑136 (MQ=255)
                                                                gTGCCGGGTTGGGGCGGTGGGCGGGGCTTGAGAAAATTGCCGCTTAACATGCTTAGCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTgg                                                            <  8:295064/136‑1 (MQ=255)
                                                                gTGCCCGGATGTGGCGGTTGGCGGTGCTGGAGCAAATTGCCTCTGAACATGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTgg                                                            <  7:282006/136‑1 (MQ=255)
                                                                  gCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGc                                                           >  3:289403/1‑135 (MQ=255)
                                                                  gCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGc                                                           >  5:310264/1‑135 (MQ=255)
                                                                   cccGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCa                                                         <  1:164193/136‑1 (MQ=255)
                                                                   cccGGATGGGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCa                                                         <  2:283929/136‑1 (MQ=255)
                                                                          gggggggttgggggtGCTTGAGAAAATTGCCGCTTAACTTGGTTTTCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGAt                                                  <  4:172840/131‑1 (MQ=255)
                                                                          ggggcggttgggggtGCTTCCGAAAATTGCCGCGTAACTTGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGAt                                                  <  7:307617/134‑1 (MQ=255)
                                                                           gggcggtttgcggtGCTTGTGAAAATTGCCGCTGAACATGATTATCGGGTGGGGGCGGTCGCTGAATCCATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGAt                                                  <  7:71135/134‑1 (MQ=255)
                                                                           gggcggttggcggtGATTTAGCAAATTGCCGCTTAAATTGCTTAGGGGGTGGGGGGGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGAt                                                  <  3:60047/134‑1 (MQ=255)
                                                                               ggttgggggtGCTTGAGCAAATTGCCGCTTAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                                             <  6:84703/136‑1 (MQ=255)
                                                                               ggtgggcggtGCTTGAGCAATTTGCCGCTTAACATGCTTTTGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                                             <  7:126526/136‑1 (MQ=255)
                                                                               gggtggcggtGGTTGACAAAATTGCCGCTGAACATGTTTATGGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCGTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                                             <  1:92050/133‑1 (MQ=255)
                                                                                    gcggtGCTTGAGAAAATTGCCGCTGAACATGCTTATGGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGctct                                        <  4:274599/136‑1 (MQ=255)
                                                                                        tGCTTGAGCAAATTGCCGATGAACATGCTTTTCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCTAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGc                                    <  6:77983/136‑1 (MQ=255)
                                                                                                  aaTTGCCGCTGAAAAGGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCt                          <  3:290477/136‑1 (MQ=255)
                                                                                                       cggcggAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCtt                     <  2:218535/131‑1 (MQ=255)
                                                                                                       ccGCTGAACTTGATTATCGGGTGGGGTCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCt                      <  8:142364/135‑1 (MQ=255)
                                                                                                       ccGCTGAACATGATTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCt                      <  4:198896/135‑1 (MQ=255)
                                                                                                             aaCATGCTTTGCGGGGGGGGGCGGGCGCGGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCTTGAGTaa               <  3:123988/136‑1 (MQ=255)
                                                                                                              aCATGCTTTTGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCTTGAGTaa               <  7:99273/135‑1 (MQ=255)
                                                                                                                   gttttgggggggggggggGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCTTGAGTAACCAgg          <  7:214960/129‑1 (MQ=255)
                                                                                                                   cTTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCTTGAGTAACCAgg          <  5:159727/135‑1 (MQ=255)
                                                                                                                   cTTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCTTGAGTAACCAgg          <  3:154349/135‑1 (MQ=255)
                                                                                                                   cTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCTTGAGTAACCAgg          <  1:162889/135‑1 (MQ=255)
                                                                                                                     tATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCTTGAGTAACCAGGAcc       >  8:278592/1‑136 (MQ=255)
                                                                                                                          ggTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCTTGAGTAACCAGGACCTtctc  >  4:268169/1‑136 (MQ=255)
                                                                                                                            |                                                                                                                                     
GTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCTTGAGTAACCAGGACCTTCTC  >  ACB143/2730025‑2730282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑