Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	3,138,350	0	T	G	40.0%	36.2 / 16.0	40	F240V (TTT→GTT)	rbsC_2	Ribose import permease protein RbsC
Reads supporting (aligned to +/- strand):  ref base T (11/13);  new base G (16/0);  total (27/13)
Fisher's exact test for biased strand distribution p-value = 3.11e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGGCA  >  ACB143/3138221‑3138484                                                                                                                                  |                                                                                                                                       acGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGtt                                                                                                                                  <  1:118172/136‑1 (MQ=255) acGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGtt                                                                                                                                  <  8:168762/136‑1 (MQ=255) acGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGtt                                                                                                                                  >  2:271752/1‑136 (MQ=255) acGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGtt                                                                                                                                  >  7:73305/1‑136 (MQ=255)      aTTCGGCCTGCGTCTGTACGCCATCGGAGCATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcg                                                                                                                             <  6:220150/136‑1 (MQ=255)      aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTgggcg                                                                                                                             >  2:331493/1‑136 (MQ=255)           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTg                                                                                                                        >  7:245457/1‑136 (MQ=255)           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTgggcgggcgg                                                                                                                        >  4:303522/1‑132 (MQ=255)           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGccgg                                                                                                                        >  3:180819/1‑134 (MQ=255)           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTAGGCGGccgg                                                                                                                        >  6:275772/1‑134 (MQ=255)           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTAGGCGGGCTg                                                                                                                        >  8:22657/1‑136 (MQ=255)                  tCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTGTTGTTCGGCGGCCGGGTTCAGc                                                                                                                 >  8:271325/1‑136 (MQ=255)                  tCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTGGGTCAGc                                                                                                                 >  1:3465/1‑136 (MQ=255)                      tACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGgc                                                                                                              <  3:303522/135‑1 (MQ=255)                         gCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTAGGCGGCCAGGTTCAGCGgcagca                                                                                                          >  5:77511/1‑136 (MQ=255)                              cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTTTTAGGCGGCCAGGGTAAGCGGCCTCaacacc                                                                                                     >  3:282412/1‑136 (MQ=255)                              cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCTGGGTCAGCGGCCGCcccacc                                                                                                     >  8:127665/1‑136 (MQ=255)                                     tACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCg                                                                                              <  1:27529/136‑1 (MQ=255)                                      aCCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTGGGCGGGCAGGTTCAGCCGCAGCACCCCCGGTTCAgg                                                                                             >  4:115895/1‑136 (MQ=255)                                        ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTGGGCGGCCGGGGTCAGCGGCAGCACCACCGGTTCCGGc                                                                                            >  2:216638/1‑135 (MQ=255)                                        ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGGTACGCGGCAGCACCCCCGGTTACGGcc                                                                                           >  6:121157/1‑135 (MQ=255)                                        ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTAGGCGGCCTGGTTCAGCCGCAGCACCACCGGGTACGGCg                                                                                           >  8:29606/1‑136 (MQ=255)                                        ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTCGGGGGCCGGGGTCAGCGGCCGCCCCCCCCGGTACGGCg                                                                                           >  5:284177/1‑136 (MQ=255)                                                 gcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtgctgc                                                                                  <  7:73870/136‑1 (MQ=255)                                                        ccGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCgt                                                                           <  6:62378/136‑1 (MQ=255)                                                              gCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTGTTGGGCGGCCTGGTTCAGCGGCCGCACCCCCGGGTCCGGCGCGATGCTGCTGTCCGGGGTGGc                                                                     >  2:318778/1‑136 (MQ=255)                                                              gCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTGTTCGGCGGGCGGGGTTAGCGGCCGCACCCCCGGGTGCGGGGAGATGGTGGTGTGCGgggtggg                                                                     >  5:246027/1‑135 (MQ=255)                                                                       tGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCttt                                                            <  5:337852/136‑1 (MQ=255)                                                                           tGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCtt                                                        <  5:223803/136‑1 (MQ=255)                                                                            ggCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTGGGCGGCCTGGTTGAGCGGCCGCCCCACCGGGTGCGGCGAGTTGCTGCTGTGCGGGGGGGGCATGGCGTttgttg                                                       >  5:224559/1‑136 (MQ=255)                                                                             tcTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTgg                                                      <  4:164319/135‑1 (MQ=255)                                                                             gCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCGGGTTCAGCGGCAGCACCCCCGGTTGCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTGTTgg                                                      >  7:69049/1‑136 (MQ=255)                                                                                aCGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTAGGCGGCCTGGTTAAGCGGCCGCACCACCCGTTCCGGCGCGATGCTGCTGTCCGGGGTGGGCAACGCTTTTATTGGGGt                                                   >  5:128184/1‑136 (MQ=255)                                                                                 cGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTg                                                  <  8:191330/136‑1 (MQ=255)                                                                                      tCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTACGGCGAGATGCTGCTGTCCGTGGTGGCCCTGGCGTTTCTTGGGGTGAtctt                                             >  8:16972/1‑136 (MQ=255)                                                                                        gCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGAtcttc                                            >  3:354608/1‑135 (MQ=255)                                                                                         ccAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTtctc                                          <  1:240262/136‑1 (MQ=255)                                                                                                       aGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTAGGCGGCCTGGTTCAGCGGCAGCACCACCGGGTGCGGCGAGATTCTGCTGGCCGGGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCCGc                            >  8:17391/1‑136 (MQ=255)                                                                                                           gTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCGGGGTAAGCGGCAGCCCCCCCGGGTGCGGCGCGATGCTGCTGTGCGTGGTGGCCATCGCGTTTGTTGGGGGGATCGTCTCCCACCCTCTGCAGCCGAg                        >  4:109396/1‑136 (MQ=255)                                                                                                                                 gTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTTCAGCCGAGCATCGGCGGCACGCTGCTggga  <  6:269180/136‑3 (MQ=255)                                                                                                                                  |                                                                                                                                       ACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGGCA  >  ACB143/3138221‑3138484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑