Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,074,834 T→A 8.0% K149* (AAA→TAA)  ohrR ← Organic hydroperoxide resistance transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,074,8340TA 8.0% 158.6 / 4.2 50K149* (AAA→TAA) ohrROrganic hydroperoxide resistance transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (28/18);  new base A (2/2);  total (30/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

GCTCCCGTTTGCAGGAGGGCGGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCACGGCGGCACCTCTTTGGCCTGCTGTTGCAGGGCGCGGCCTTTGTC  >  ACB143/2074708‑2074963
                                                                                                                              |                                                                                                                                 
gcTCCCGTTTGCAGGAGGGCGGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCTCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtt                                                                                                                          >  5:169055/1‑136 (MQ=255)
gcTCCCGTTTGCAGGAGGGCGGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtt                                                                                                                          >  5:136316/1‑136 (MQ=255)
gcTCCCGTTTGCAGGAGGGCGGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtt                                                                                                                          >  4:136057/1‑136 (MQ=255)
gcTCCCGTTTGCAGGAGGGCGGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTACTGCAGGtt                                                                                                                          >  3:181224/1‑136 (MQ=255)
gcTCCCGTTTGCAGGAGGGCGGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTACTGCAGGtt                                                                                                                          >  3:184861/1‑136 (MQ=255)
  tCCCGTTTGCAGGAGGGCGGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCTCCGTTCATCGGAACGGCCATTTCACAGAATAGAGCTCAGAGGTTTTTCTGCAGGttat                                                                                                                        >  7:252792/1‑134 (MQ=255)
    ccGTTTGCAGGAGGGCGGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtttt                                                                                                                        <  6:136316/134‑1 (MQ=255)
    ccGTTTGCAGGAGGGCGGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtttt                                                                                                                        <  5:31615/134‑1 (MQ=255)
    ccGTTTGCAGGAGGGCGGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtttt                                                                                                                        <  3:156217/134‑1 (MQ=255)
     cGTTTGCAGGAGGGCGGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTcgc                                                                                                                     >  4:167464/1‑136 (MQ=255)
                           ggTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCtt                                                                                                <  5:236104/135‑1 (MQ=255)
                               tgGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGt                                                                                           >  7:26099/1‑136 (MQ=255)
                                    tttAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCATCAGGTCTTCTTGCAGTtgctg                                                                                      <  3:167464/136‑1 (MQ=255)
                                    tttAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTtgctg                                                                                      >  3:252980/1‑136 (MQ=255)
                                          ttAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCTTTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTg                                                                                <  4:232974/136‑1 (MQ=255)
                                                   aaTTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCt                                                                       >  1:8849/1‑136 (MQ=255)
                                                      tagttagtGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTccg                                                                    >  4:7620/1‑136 (MQ=255)
                                                            gtGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCt                                                              <  4:252980/136‑1 (MQ=255)
                                                                gcAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtttt                                                                                                                        >  4:119569/1‑74 (MQ=255)
                                                                gcAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtttt                                                                                                                        <  3:119569/74‑1 (MQ=255)
                                                                gcAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGcc                                                          >  7:121966/1‑136 (MQ=255)
                                                                gcAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGcc                                                          >  2:285751/1‑136 (MQ=255)
                                                                gcAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGcc                                                          >  2:289675/1‑136 (MQ=255)
                                                                gcAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGcc                                                          >  4:211671/1‑136 (MQ=255)
                                                                gcAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGcc                                                          >  5:153405/1‑136 (MQ=255)
                                                                gcAAGATAAATCATCGCCATTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGcc                                                          >  3:43176/1‑136 (MQ=255)
                                                                 cAAGATAAATCATAGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTTCTGCAACTGCTCAAGGATCCGCCCGCTGGCCt                                                         >  7:267306/1‑136 (MQ=255)
                                                                  aaGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGAAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCtt                                                        >  8:258122/1‑136 (MQ=255)
                                                                    gATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTAAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCtt                                                        >  3:81185/1‑134 (MQ=255)
                                                                     aTAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCtt                                                        >  2:57531/1‑133 (MQ=255)
                                                                      tAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTgag                                                     >  8:150689/1‑135 (MQ=255)
                                                                                ccGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCAcggcg                                          <  3:7620/136‑1 (MQ=255)
                                                                                ccGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCAcggcg                                          <  8:121966/136‑1 (MQ=255)
                                                                                 cGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCAcggcgg                                         >  5:158455/1‑136 (MQ=255)
                                                                                      aTCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCg                                                                                                    >  6:269467/1‑72 (MQ=255)
                                                                                      aTCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCg                                                                                                    <  5:269467/72‑1 (MQ=255)
                                                                                           aaCGGCCATTTCACAGCATATCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCGCGGCGGCACCTCTTTg                               <  8:130/136‑1 (MQ=255)
                                                                                           aaCGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCACGGCGGCACCTCTTTg                               >  4:19916/1‑136 (MQ=255)
                                                                                           aaCGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGGTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCGCGGCGGCACCTCTTTg                               <  8:129/136‑1 (MQ=255)
                                                                                             cGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTATGCAGGTTTTCTCGCAGGTTCAGCAGGTCGTCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCACGGCGGCACCTCTTTGGc                             <  6:169055/136‑1 (MQ=255)
                                                                                                          gCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTGCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCACGGCGGCACCTCTTTGGCCTGCTGTTGCAgg                <  1:274251/136‑1 (MQ=255)
                                                                                                                 gcTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCACGGCGGCACCTCTTTGGCCTGCTGTTGCAGGGCGCGGc         >  5:38191/1‑136 (MQ=255)
                                                                                                                 gcTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCACGGCGGCACCTCTTTGGCCTGCTGTTGCAGGGCGCGGc         >  6:86617/1‑136 (MQ=255)
                                                                                                                 gcTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCACGGCGGCACCTCTTTGGCCTGCTGTTGCAGGGCGCGGc         >  3:44613/1‑136 (MQ=255)
                                                                                                                        ggTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCGGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCACGGCGGCACCTCTTTGGCCTGCTGTTGCAGGGCGCGGCCTTTGTc  >  3:219297/1‑136 (MQ=255)
                                                                                                                        ggTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCACGGCGGCACCTCTTTGGCCTGCTGTTGCAGGGCGCGGCCTTTGTc  <  2:154925/136‑1 (MQ=255)
                                                                                                                        ggTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCACGGCGGCACCTCTTTGGCCTGCTGTTGCAGGGCGCGGCCTTTGTc  <  7:85857/136‑1 (MQ=255)
                                                                                                                        ggTTTTACTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCACGGCGGCACCTCTTTGGCCTGCTGTTGCAGGGCGCGGCCTTTGTc  <  4:184861/136‑1 (MQ=255)
                                                                                                                        ggTTTTACTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCACGGCGGCACCTCTTTGGCCTGCTGTTGCAGGGCGCGGCCTTTGTc  <  4:181224/136‑1 (MQ=255)
                                                                                                                         gTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCACGGCGGCACCTCTTTGGCCTGCTGTTGCAGGGCGCGGCCTTTGTc  <  2:280402/135‑1 (MQ=255)
                                                                                                                              |                                                                                                                                 
GCTCCCGTTTGCAGGAGGGCGGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCCGCCCGCTGGCCTTGAGAATGCACGGCGGCACCTCTTTGGCCTGCTGTTGCAGGGCGCGGCCTTTGTC  >  ACB143/2074708‑2074963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑