Predicted mutation
evidence seq id position mutation annotation gene description
MC JC ACB143 5,389,996 Δ8 bp coding (90‑97/1666 nt) ligH → 10‑formyltetrahydrofolate synthetase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ ACB143 5389996 5390003 8 50 [0] [0] 50 ligH 10‑formyltetrahydrofolate synthetase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 = 53899950 (0.000)50 (0.820) 33/262 0.5 100% coding (89/1666 nt) ligH 10‑formyltetrahydrofolate synthetase
?ACB143 5390004 = 0 (0.000)coding (98/1666 nt) ligH 10‑formyltetrahydrofolate synthetase

GGCGACCTGCCCGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/5389863‑5389995
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCA  >  ACB143/5390004‑5390135
                                                                                                                                                                                                                                                                         
GGCGACCTGCCCGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGA                                                                                                                                   <  7:118437/136‑1
     CCTGCCGGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTAC                                                                                                                              <  6:201375/136‑1
     CCTGCCCGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTAC                                                                                                                              <  8:299932/136‑1
      cgcCCCGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTAC                                                                                                                              <  3:285865/132‑1
      CTGCCCGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTAC                                                                                                                              <  3:73882/135‑1
                  GCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAG                                                                                                                  <  5:191175/135‑1
                     ACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCA                                                                                                              <  1:67961/136‑1
                      CGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGC                                                                                                                >  4:190234/1‑133
                           AAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGA                                                                                                        <  2:94734/136‑1
                           AAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTG                                                                                                         <  7:341049/135‑1
                                 GAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGG                                                                                                   <  4:153721/135‑1
                                 GAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGG                                                                                                   <  3:3716/135‑1
                                          ACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCA                                                                                         <  8:155600/136‑1
                                          ACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCA                                                                                         <  1:152177/136‑1
                                          ACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCA                                                                                         <  7:213586/136‑1
                                               GCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGC                                                                                    <  1:217337/136‑1
                                               GCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAG                                                                                     >  3:170826/1‑135
                                                CACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGC                                                                                    <  3:233277/135‑1
                                                 ACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGT                                                                                  <  7:161627/136‑1
                                                 ACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGT                                                                                  >  2:298686/1‑136
                                                    AGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTT                                                                                 >  2:99138/1‑134
                                                      GCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGG                                                                             >  8:264036/1‑136
                                                         CCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGCACTACGGCCCCTACAAGGCCAAGCTGACCTGGGACTTCATCAACAGCGGTCAGGG                                                                            >  4:251500/1‑134
                                                             TATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAAC                                                                        >  5:322690/1‑134
                                                             TATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAAC                                                                        >  6:236214/1‑134
                                                             TATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAAC                                                                        >  2:235057/1‑134
                                                                      CGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCA                                                              <  4:230529/135‑1
                                                                       GCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAG                                                            >  1:107657/1‑136
                                                                         CCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCT                                                          <  2:260318/136‑1
                                                                         CCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGC                                                           >  3:356874/1‑135
                                                                                         CAGAATCTCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGC                                           <  8:90733/135‑1
                                                                                         CAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGC                                           <  6:292858/135‑1
                                                                                         CAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGC                                           <  8:61332/135‑1
                                                                                            AATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGAT                                        >  5:289361/1‑135
                                                                                            AATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGAT                                        >  8:37243/1‑135
                                                                                                    GGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGCCTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCACACCGAC                               >  6:108684/1‑136
                                                                                                    GGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGAC                               >  6:332995/1‑136
                                                                                                    GGAGGTCGGCGCGAAAGACGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGATCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGAC                               >  2:15078/1‑136
                                                                                                      AGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAGCTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGC                             >  5:274534/1‑136
                                                                                                      AGGTCGGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGC                             <  8:209682/136‑1
                                                                                                      AGGGCGGCGCGAAAGTCGGCATCACGCAGGATGAACTACGGCCCCTACAAGGCAAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTTTTCCTCGTCACCGCGATCAACCCGACGC                             <  1:137636/136‑1
                                                                                                           GGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGC                         >  3:237835/1‑135
                                                                                                           GGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGAC                                                                  >  8:229374/1‑94
                                                                                                           GGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGAC                                                                  <  7:229374/94‑1
                                                                                                           GGCGCGAAAGTCGGCATCCCGCAGGATGAACTACGCCCCCTACAAGGCAAAGCTGAGCTGGGACTTCATCAACAGCGGTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACAGCTATCAACCCGACGCCGGC                         >  3:55911/1‑135
                                                                                                            GCGCGAAAGTCGGCATCCCGCAGGATGAACTACGGCCCCTACCACGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCG                       <  2:135644/136‑1
                                                                                                                  AAGTCGGCATCCCGCAGTATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGAAACCAGGACGGCAAGCTGATCCTCGTCACCGCTATCAACCCGACGCCGGCCGGCGAAG                 <  7:265474/136‑1
                                                                                                                   AGTCGGCATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAA                  <  3:233120/134‑1
                                                                                                                          ATCCCGCAGGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCACCCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGAC          >  4:311603/1‑135
                                                                                                                                  GGATGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCA  >  3:2109/1‑135
                                                                                                                                                                                                                                                                         
GGCGACCTGCCCGGGCTCGCCACGAGAAAGGACGAGGAGGACACATGGCACAAGGCTCCGATATCGAGATCGCCCGCGAAGCGAAGATGCAGAATATCGCGGAGGTCGGCGCGAAAGTCGGCATCCCGCAGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/5389863‑5389995
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGAACTACGGCCCCTACAAGGCCAAGCTGAGCTGGGACTTCATCAACAGCGTTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCA  >  ACB143/5390004‑5390135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.