| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 2,668,473 | 0 | A | C | 57.9% | ‑0.1 / 11.4 | 19 | G234G (GGT→GGG) | CJ019_02525 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (4/4); new base C (11/0); total (15/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGGCCTTGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACAGCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGCCGGCGCCGGCACCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTGGTCAAGTCCAGCGGCAGGTAGCGTGAGGGGAAGTCGGTCAGCGTGTCGAGCGGAGGCGCGGCG > ACB143/2668341‑2668608 | cagggcCTTGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACAGCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGCCGGCGCCGGCAccc > 6:308193/1‑135 (MQ=255) cTTGTCGACCCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACAGCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGCCGGCGCCGGGCCCGGGGGTg > 7:36202/1‑136 (MQ=255) cTTGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACAGCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGGCGGCGGCGGCCCCgggggt > 4:218263/1‑133 (MQ=255) cTTGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACAGCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGCCGGCGCCGGCACCGGGGATg > 6:267414/1‑136 (MQ=255) tGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACAGCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGCCGGCGCCCGCCCCcggggggt > 4:65467/1‑131 (MQ=255) gAACCCGCGGGGAACCACCTTGGCGGTTAAGGGCGGGACCTGCACCAGGGCGGCGCCGCGATAGGGCTGGGCGCGGGTATAGTCGAGCAGGCCGCCGGCGCGTTAGGTGCCGGCGCCGGCACCGGGGATGTCTTTc < 8:296343/136‑1 (MQ=255) cAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACAGCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTTGCGGCGCCGGCCCCCGGGCTGTCATTCaacc > 6:58772/1‑133 (MQ=255) ccAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGGCCGCGCCCGCCCCGGGGGGGTGGTTGACGCCCAGCCTGACCCTAGGTTTGGGCCCCGGAGCGCGCAGGGc > 1:200015/1‑136 (MQ=255) cgtcgCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGCCGGCGCCGGCCCCCGGGATGTCTTTCCCGCCCTGCATGCCCATCGGGATCGACCCGGGAGCGAGCAgggcggggcg > 8:69738/1‑136 (MQ=255) cgtcgCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGCCCGCGCCCGCCCCGGGGGTGTGTTTAACCCCCAGCATGACCATCGGTATAGCCCCGGAAGCGCGCCgggcggggcg > 1:237895/1‑136 (MQ=255) cgtcgCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGCCCGCGCCCGCCCCGGGGGAGTATTTAACGCCCCGCATGACCAACGGGATCGCCCCCGCAGCGAGCCCGGCGGGgcg > 8:48056/1‑136 (MQ=255) ggcgccgcGATAGGGCTGGTCGAGGTGATAGTCGAGCAGGCCGCGGGCGCGTTAGGGGCCGGCGCCGGCACCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGgcgc < 8:124159/134‑1 (MQ=255) ccgcGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGCCGGCGCCGGCACCGGGGGTGTCTTTGACCCCCGGCATGACCCTCGGGATAGGCCCGGCAGCGCGCAGGGCGGGGGGCacgg > 8:87671/1‑133 (MQ=255) ccgcGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGCCCGCGCCCGGCCCCGGGATGTGTTTAAACCCCCGCAATACCAACGGGATAGGCCCCGCAGCGCGCAGGGCGTGGGGGAgg > 3:116057/1‑135 (MQ=255) gAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGCCCGCGCCGGCCCCGGGGATGTATTTCAAGCCCAGCAAGACCATCGGTATAGACCCCGAAGCGAGCAGGGCGGGGGGCAGGTTGGTCAAGTCCAGCGGc > 1:79458/1‑136 (MQ=255) gccgTCGCGGTAGGTGCCGGCGCCGGCCCCGGGGGTGTCTTTAACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGCGCAGGGCGGGGCGGCGGGTGGTCAAGTCCCGCGGCAGGGTGCGGGAGGGGAAGTCg > 8:38209/1‑136 (MQ=255) ggggcgggcgcgggcACCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTGGTCAAGTCCAGCGGCAGGTAGCGTGAGGGGAAGTCGGTCAGCGTGTc < 2:237895/129‑1 (MQ=255) cggccccgccACCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTGGTCAAGTCCAGCGGCAGGTAGCGTGAGGGGAAGTCGGTCAGCGTGTCGAGCgg < 6:224865/136‑1 (MQ=255) cgccggcACCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTGGTCAAGTCCAGCGGCAGGTAGCGTGAGGGGAAGTCGGTGAGCGTGTCGAGCGGAgg > 5:157362/1‑136 (MQ=255) aCCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCTGGGCGTGGCGCAGGTTGGTCAAGTCCAGCGGCAGGGAGCGTGAGGGGAAGTCGGTCAGCGTGTCGCGCGGAggcgcggcg > 5:309456/1‑136 (MQ=255) aCCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTGGTCaa > 2:86208/1‑78 (MQ=255) aCCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTGGTCaa < 1:86208/78‑1 (MQ=255) aCCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTGGTCAAGTCCAGCGGCAGGTAGCGTGAGGGGAAGTCGGTCAGCGTGTCGAGCGGAggcgcggcg > 1:207096/1‑136 (MQ=255) aCCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTGGTCAAGTCCAGCGGCAGGTAGCGTGAGGGGAAGTCGGGCAGCGTGTCGAGCGGAggcgcggcg > 7:180783/1‑136 (MQ=255) aCCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGAGGAGCAGGTTGGTCAAGTCCACCGGCAGGTAGCGGGAGGGGAAGTCGGTGAGCGTGTCCCGCCGcggcgcggcg > 8:295770/1‑136 (MQ=255) | CAGGGCCTTGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACAGCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGCCGGCGCCGGCACCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTGGTCAAGTCCAGCGGCAGGTAGCGTGAGGGGAAGTCGGTCAGCGTGTCGAGCGGAGGCGCGGCG > ACB143/2668341‑2668608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |