| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 3,923,469 | 0 | A | C | 52.2% | 2.6 / 13.9 | 23 | W423G (TGG→GGG) | CJ019_03707 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (8/3); new base C (12/0); total (20/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.32e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCACCCCCTGCAAATGCCAAACCCCCAGCGGCTGGTCCGC > ACB143/3923344‑3923604 | cGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCCCCGCCGCCCg > 1:216894/1‑136 (MQ=255)cGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCAGCGCCCCCACCCCCCGCCGCCCg > 7:134584/1‑136 (MQ=255)cGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGCGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCCATCTCAAACCACCCCCGGCTCCCCCCGCCCCCcccgcccg > 5:115358/1‑136 (MQ=255) gCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCCGGCCCCGccgcccgc > 5:142837/1‑136 (MQ=255) aGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCCGCCCCCCccgcccgcgc > 7:35005/1‑134 (MQ=255) aGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCAGCTCCCCCCCCCCCCGccgcccgcgc > 5:336107/1‑134 (MQ=255) tgggtgggCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCAGCTCCCCCAGCCACCGCCGCACGctcctc > 5:309900/1‑136 (MQ=255) tgggtgggCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCAGCGCCCCCCGGCACCGCCGCCCGcgcctg > 8:134322/1‑135 (MQ=255) tgggtgggCGGGATACCGGGACTTACCTGCAATTGATTGTCGCGACCTATTTCTGATACGACATCCTGGGGCTGCGAAGAACCACCGGCAATCTCAAACCAACACATGCTCCCCCAAACACCCCCCCACGctccgc > 2:250959/1‑134 (MQ=255) ggtgggCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCCGCCACCGCCGCACGCGCcgct > 4:143634/1‑135 (MQ=255) tGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCCGCCCCCGCCGCACGCGCCTCTGCGGTTACCCCGCAAGCCCCcaca > 4:248541/1‑134 (MQ=255) tGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCGCCCCCCGCCCCCCCCGCCCGCGCCGCTGCGGGTCCCCCGCAAGcccccccca > 5:62146/1‑130 (MQ=255) ggACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCCGCCCCCCCCGCACGCTCCTCAGCTGGTCACCCGCCAGCCCCGACAATACCGCCTCACCCGgc > 8:194040/1‑136 (MQ=255) gACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCGCCCCCCCCCCCCGCCGCCCGCGGCGCGGGGGGTGACCCGCCAGCCCCCCCAATACCGCCACAAAACgcc > 6:39532/1‑136 (MQ=255) cTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCCCCGCCCGCTCCTCTGCTGTTCCCCCGCCAGCCCCGACAAAACCGCCTCAACAGGCCGCCCCCTGc > 1:103816/1‑136 (MQ=255) cTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCACCCACCGCCGCACGCTACCCTCCTGTTAACCAGCAAGCCCCCACAAAACCGCCTAAACAGGCCGCCCAcacc > 1:103819/1‑133 (MQ=255) cGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCCCCGCCGCCCGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGGAttt > 1:130806/1‑136 (MQ=255) gACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCAGCTCCCCCAGCCCCCGCCGCACGCTCCTCTGCTGTTACCCCGCAAGCCCCGCCAATACCGCCTCAACAGGCCGCCCACTGCGTAttt > 3:60178/1‑135 (MQ=255) cccccccGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGtt < 2:228803/133‑1 (MQ=255) cccccGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTcc < 6:118250/135‑1 (MQ=255) aaaCCAACCCCTGCTCCCCCAGCCAACGACGCACGCTCATCTGCTGTTCACAAGCAAGCCCAGACAATACCGCATAAACAGGCCGACCAATGCTTATTTTTCGCACAACCGGAGCCAGTTAAAACCCCTGAAAAt > 2:238440/1‑135 (MQ=255) aaccaaccCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCACCCCCTGCAAATGc < 8:275096/136‑1 (MQ=255) gCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCACCCCCTGCAAATGCCAAACCCCCACCGGCTGGTc > 8:301912/1‑136 (MQ=255) aCCGCCGCACGCTCCTCTGCTGTTCCCCCGCCAGCCCCGACAATACCGCCTCAACAGGCCGCCCACGGCGTATTTGTCGCACCACCGGCGCCAGTTCCCCCCCCTGCAAATGCCAAACCCCCCGCGGCTGGTccgc > 5:33728/1‑136 (MQ=255) aCCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCCCCCCCGGCCAATGCCAAACCCCCAGCGGCTGGTccgc > 5:300894/1‑136 (MQ=255) aCCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCACCCCCTGCAAATGCCAACCCCCCAGCGGCTGGTccgc > 1:77110/1‑136 (MQ=255) | CGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCACCCCCTGCAAATGCCAAACCCCCAGCGGCTGGTCCGC > ACB143/3923344‑3923604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |