Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,125,6970GC36.8% 53.6 / 10.6 39F115L (TTC→TTG) yadVputative fimbrial chaperone YadV
Reads supporting (aligned to +/- strand):  ref base G (18/6);  new base C (0/14);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 4.32e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GATCGGTCGGCGTACCCTTGAGTGTGGGCGGCCGATAGAACAGTTTGATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACAGCGGTGGGGACGTTGCAAAGGGTACGCTGGTCGTATTGTCGTCGGCGTCCG  >  ACB143/2125573‑2125833
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gATCGGTCGGCGTACCCTTGAGTGTGGGCGGCCGATAGAACAGTTTGATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGGCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAACAACAACAGCGACTcg                                                                                                                              <  8:118379/137‑1 (MQ=255)
    ggtcggCGTACCCTTGAGTGTGGGCGGCCGATAGAACAGTTTGATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGt                                                                                                                          >  4:369128/1‑137 (MQ=255)
     gtcggCGTACCCTTGAGTGTGGGCGGCCGATAGAACAGTTTGATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTc                                                                                                                         >  3:213587/1‑137 (MQ=255)
          gttACGCTTGTGTGTGGGCGGCCGATATAACTGTTTGTTGCGTGTGCTCACGGCTATGTTGATTTTATTGCCTGTGTCGCTGCTGAGTTGCGTAATCTCCTGCACGTTCAAGAACACCAGCGACTCGCGGTCGtgtg                                                                                                                    <  4:329929/135‑1 (MQ=255)
          gtaaCCGTTGTGTGTGGGCGCCCTATAGAACTGTTTTTGGGGTGTGCGAAAGGCTTTGTTTAGTTGATTGCCTGTGTCGCTGCTCACTTGGGCAAGCCCCTCCCCGTTCAACAACACCCGCGACTCGCGGTCGtgtg                                                                                                                    <  4:13693/134‑1 (MQ=255)
          ggaaCCCTTGTGGGGGGGCGGCCTATAGAACTTTTGGAGGCGGGGGCGCAAGGGGTTGTTGAGTTGATTGCCCGTGGCGCTGCTGACTTGCGGAATCTCCTCCAGGTTGACGAACAACAGCGACTCGCGGTCGtgtg                                                                                                                    <  6:48709/134‑1 (MQ=255)
          cGTACCGTTGCGTGGGGGCGCCCGATAGAACAGTTTGATGCGTGTGCGCAAGGCTTTGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGGGTAATCTCCTGCACGTGGAACAAGAACAGCGACTCGCGGTCGtgtg                                                                                                                    <  4:366998/137‑1 (MQ=255)
          cGTACCCTTGAGTGTGGGCGGCCGATAGAACAGTTTGATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAACAACAACAGCGACTCGCGGTCGtgtg                                                                                                                    <  1:39780/137‑1 (MQ=255)
          cGTACCCTTGAGTGTGGGCGGCCGATAGAACAGTTTGATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTCAAGAACAACAGCGACTCGCGGTCGtgtg                                                                                                                    <  1:210783/137‑1 (MQ=255)
           gTACCCTTGAGTGTGGGCGGCCGATAGAACAGTTTGATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTgg                                                                                                                   >  2:200259/1‑137 (MQ=255)
           gTACCCTTGAGTGTGGGCGGCCGATAGAACAGTTTGATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTgg                                                                                                                   >  7:276482/1‑137 (MQ=255)
                       gtgGGCGGCCGATAGAACAGTTTGATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTggg                                                                                                       >  5:81464/1‑137 (MQ=255)
                         gggCGGCCGATAGAACAGTTTGATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCa                                                                                                     >  7:266223/1‑137 (MQ=255)
                           gcggcCGATAGAACAGTTTGATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGt                                                                                                   >  3:135302/1‑137 (MQ=255)
                            cggcCGATAGAACAGTTTGATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGta                                                                                                  >  5:339417/1‑136 (MQ=255)
                            cggcCGATAGAACAGTTTGATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTc                                                                                                  >  8:246839/1‑137 (MQ=255)
                                              gATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCTCGTTGAAGAAGAACAGCGACTCGCGGTCGTGGGGCAGTGTATTGGGCAGGCGAATTTTTTTTTGATgtt                                                                                >  3:342758/1‑137 (MQ=255)
                                              gATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCgtgt                                                                                                                     <  7:123512/100‑1 (MQ=255)
                                              gATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCgtgt                                                                                                                     >  8:123512/1‑100 (MQ=255)
                                              gATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTTGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAgtg                                                                                >  7:84195/1‑136 (MQ=255)
                                                tGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAgttgt                                                                              <  4:129624/137‑1 (MQ=255)
                                                tGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCACTCAAAATATTTATAGAAgtttt                                                                              >  2:103606/1‑135 (MQ=255)
                                                        cgcAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGtt                                                                      >  7:103751/1‑137 (MQ=255)
                                                          cAAGGCGATGTTGTGTTGATTGCCTGTGGCGCTGGTGACTTGCGTAATCTCCTGCACGTTGAAGAACAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCg                                                                    <  3:369128/137‑1 (MQ=255)
                                                          cAAGGCGATGTTGATTTGCTGGCCTGTGTCGCTGCGAACTGGCGGAAGCTCCTCCACGTTTAAGACCAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGAAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCg                                                                    <  8:120345/137‑1 (MQ=255)
                                                                gATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTCTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTAGGGTTGa                                                              >  7:176425/1‑137 (MQ=255)
                                                                  tGTTGATTTTATTGCCCGGGTCGCTGCTGAGTGGCGGAATCTCCTCCACTTTCAATACTAACCGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATc                                                            <  4:253332/137‑1 (MQ=255)
                                                                    ttgagttgaTTGCCAGTGGCGCTGCTGACTTGGGGAATCTCCTGCACGTTTAAGAACAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCtt                                                          <  6:360035/137‑1 (MQ=255)
                                                                    ttgagttgaTTGCCAGGGTCGGGGCTGACTTGCGGAATCTCCTGCACGTTCAACAAGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCtt                                                          <  3:42021/137‑1 (MQ=255)
                                                                               gCCAGTGTCGAGGCTGATTTGCGTAATCTCCTGCACGTTGATGTCGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACAGCGGTg                                               <  8:266223/137‑1 (MQ=255)
                                                                                      gcggtgctgAGTTGCGTAATCTCCTCCACGTTGAATATCAACCGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACAGCGGTGGGGACGt                                        <  7:246839/133‑1 (MQ=255)
                                                                                      gcGCTGCTGACTTGCGGAATCTCCGGCAGGTTGAAGAACAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACAGCGGTGGGGACGt                                        <  6:356783/136‑1 (MQ=255)
                                                                                       ggctgctGACTTGCTGAATCTCGTGCACGTTGAAGAACAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACAGCGGTGGGGACGtt                                       <  4:213587/136‑1 (MQ=255)
                                                                                       ggctgctGACTGGCGTAATCTCCTGCACTTTTAATAAGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACAGCGGTGGGGACGtt                                       <  3:366549/136‑1 (MQ=255)
                                                                                       cGCTGCTTACTTGCGGAATCTCATGCAGGTTGAAGAACAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACAGCGGTGGGGACGtt                                       <  1:200259/137‑1 (MQ=255)
                                                                                             tgACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACATCGGTGGGGACGTTGCaaat                                 >  3:23899/1‑136 (MQ=255)
                                                                                             tgACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACAGCGGTGGGGACGTTGCAAAg                                 >  5:358577/1‑137 (MQ=255)
                                                                                                  tGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGGGGCAGTGTATTTGGCAGTCCAATTATTTTTATAAGTTGTTGACTGTTCGGGTTGATCTTGAAAAGCGGTGGGGACGTTGCAAAGGGTAc                            >  3:55285/1‑137 (MQ=255)
                                                                                                    gggAATCTCCTGCACGTTCAAGAACAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACAGCGGTGGGGACGTTGCAAAGGGTACGc                          <  4:23899/136‑1 (MQ=255)
                                                                                                                      taagaataaCAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACAGCGGTGGGGACGTTGCAAAGGGTACGCTGGTCGTATTGTCGTCgg        <  6:339417/136‑1 (MQ=255)
                                                                                                                      gaagaagaaCAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACAGCGGTGGGGACGTTGCAAAGGGTACGCTGGTCGTATTGTCGTCgg        <  8:103751/137‑1 (MQ=255)
                                                                                                                        agaagaaCAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACAGCGGTGGGGACGTTGCAAAGGGTACGCTGGTCGTATTGTCGTCGGCg      >  4:166834/1‑137 (MQ=255)
                                                                                                                         gaagaaCAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACATCGGTGGGGACGTTGCAAAGGCTACGCTGGTCGTATTGTAGTCGGCGt     >  6:341514/1‑137 (MQ=255)
                                                                                                                            gaaCAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACAGCGGTGGGGACGTTGCAAAGGGTACGCTGGTCGTATTGTCGTCGGCGTCCg  <  6:81464/137‑1 (MQ=255)
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GATCGGTCGGCGTACCCTTGAGTGTGGGCGGCCGATAGAACAGTTTGATGCGTGTGCGCAAGGCGATGTTGAGTTGATTGCCAGTGTCGCTGCTGACTTGCGGAATCTCCTGCACGTTGAAGAAGAACAGCGACTCGCGGTCGTGTGGCAGTGTATTGGGCAGTCCAATTATTTTTAGAAGTTGTTCACTGTTCGGGTTGATCTTGAACAGCGGTGGGGACGTTGCAAAGGGTACGCTGGTCGTATTGTCGTCGGCGTCCG  >  ACB143/2125573‑2125833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑