Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,730,1210CA27.6% 91.3 / 14.4 58Q1327K (CAA→AAA) CJ019_02576hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (21/21);  new base A (0/16);  total (21/37)
Fisher's exact test for biased strand distribution p-value = 1.82e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACGCCACCGCACGTTTCGCCGACCTGGAACTGCGAGTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGAT  >  ACB143/2729989‑2730254
                                                                                                                                    |                                                                                                                                     
aaCGCCACCGCACGTTTCGCCGACCTGGAACTGCGAGTGATGATATGGCGCTCGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAAt                                                                                                                                   >  7:292714/1‑137 (MQ=255)
     cACCGCACGTTTCGCCGACCTGGAACTGCGAGTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCg                                                                                                                              >  4:63829/1‑137 (MQ=255)
       ccGCCCGTTTCGCCGACCTGGAACTGCGAGTGATGATAAGGCGCACGATGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCt                                                                                                                            <  4:58585/137‑1 (MQ=255)
           aCGTTTCGCCGACCTGGAACTGCGAGTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAAc                                                                                                                        >  1:118314/1‑137 (MQ=255)
           aCGTTTCGCCGACCTGGAACTGCGAGTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAAc                                                                                                                        >  1:326298/1‑137 (MQ=255)
           aCGTTTCGCCGACCTGGAACTGCGAGTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAAc                                                                                                                        >  1:57747/1‑137 (MQ=255)
               ttCGCCGACCTGGAACTGCGAGTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGc                                                                                                                    >  4:31671/1‑137 (MQ=255)
               ttCGCCGACCTGGAACTGCGAGTGATGATAAGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGc                                                                                                                    <  1:321306/137‑1 (MQ=255)
                tCGCCGACCTGGAACTGCGAGTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGGGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCt                                                                                                                   <  4:21578/137‑1 (MQ=255)
                           aaccTGCGAGTGATGATATGGCGCACGCGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGGGCCCGGATGTGGCGGTTGGGGTTGCTTGAGAAAATTGCCGCTTAACATGATTATCGgggggg                                                                                                        <  6:270668/134‑1 (MQ=255)
                                    gTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGgcgc                                                                                               >  7:52444/1‑137 (MQ=255)
                                    gTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGgcgc                                                                                               >  5:137848/1‑137 (MQ=255)
                                        tgatATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGaa                                                                                           >  4:12922/1‑137 (MQ=255)
                                              ggCGCACGCGGCACAGCGGATGTGCCCGGCCTGAGCGGGCCTTGCTGACGCGGGGTGCCGGTATGGGGGGGTGGGCGGTGGTTGAGAAATTTGCCGCTTAACATGGTTTTCGGGTGGGGGCGGGCGCTGAatcgatc                                                                                     <  4:321595/137‑1 (MQ=255)
                                              ggCGCACGAGGCACCGCGGAGCTGCCCGGCGTGAGGGGGCCTTGCTTAAGCTCGGTGCCCGTTTGGGGGGGTTGGCGGTGCTTTAGAAAATTGCCGCTTAACTTGCTTATGGGGGGGGGGCGGGCGCTGAatcgatc                                                                                     <  2:68864/137‑1 (MQ=255)
                                                    cccggCACCGCGGATCTGCCCGGCGTGAGCGGGCCTTGCTTAAGGTGGGTGCCGGGTTGGGGGGGTTGGCGGTGCTTGAGAAAATTGCGGCTGAACATGGTTATGGGGTGGGGGCGGGCGCTGAATCGATCGAATTc                                                                               <  2:57747/134‑1 (MQ=255)
                                                     gAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCg                                                                              >  3:102270/1‑137 (MQ=255)
                                                     gAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCg                                                                              >  7:44427/1‑137 (MQ=255)
                                                       ggCACAGCGGATCTGCCCGGCCTGCGCGGGCCTTGCTGAAGCTCGGGGCCCGGATGTGGCGGTTGGCGGTGCTTGAGAAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGcc                                                                            <  3:2126/137‑1 (MQ=255)
                                                        gCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGCTGGGGGCGGGCGCTGAATCGATCGAATTCGCCt                                                                           >  8:209147/1‑137 (MQ=255)
                                                               ggATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGGGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCtt                                                                    >  1:376931/1‑136 (MQ=255)
                                                                 aTCTGCCCGGCGTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGGGGGGGTGGGCGGTGCTTCAGCAAATTCCCGCTGAACATGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATc                                                                  <  5:277234/137‑1 (MQ=255)
                                                                    tGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGaa                                                               >  7:248821/1‑137 (MQ=255)
                                                                    tGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGaa                                                               >  7:360676/1‑137 (MQ=255)
                                                                      ccgggCCGGCGCGGGCCTTGGTTAAGCGGGGGGCCGGTTGGGGGGGGTTGGGGGTGCTTTAGAAAATGGCCGCTCAACATCATTAGGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATc                                                             <  1:22892/134‑1 (MQ=255)
                                                                             tGAGGGGGCCTTGCTGACCCTCGGTGCCCGGATGGGCCGGTTGGCGGTGCTTCAGCAAATTCCGGCTCAACATCCTTATGGGGTGGGGCCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTgc                                                      <  8:342914/137‑1 (MQ=255)
                                                                             tGAGCGGGCCTTGCTGAAGCTGGGTGCCGGTTTGTGGGGGTTGGGGGTGCTTTACAAATTTGCCGCTTAACATGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTgc                                                      <  4:122624/137‑1 (MQ=255)
                                                                             tGAGCGGGCCTTGCTGAAGCTGGGTGCCCGGATGTGGCGGTTGGCGGTGCTTTAGAAATTTGCCGCTTAACATGATTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTgc                                                      <  3:12922/137‑1 (MQ=255)
                                                                             gtaGCGGGCGTTGGTTACGCTGGGTGCCGGTTTTTGGGGGTTGGCGGTGCTTGAGAAAATTGCCGCTTAACATGGTTTGGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTgc                                                      <  2:34518/135‑1 (MQ=255)
                                                                             ggAGCGGGCCGTGCGTAAGGGCGGGGCCCGGTTGTGGGGGGTGGCGGTGGTTGAGCAAATTGCCGCTGAACAGGCTTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTgc                                                      <  8:277766/136‑1 (MQ=255)
                                                                              gAGCGGGCCTTGCTGAAGCTCGGTGCCCGGTGGTGGCGGTTGGGGGTGGTTGAGCAAATTGCCGCTTAACATGCTTATGGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTgcg                                                     <  3:228197/137‑1 (MQ=255)
                                                                                       ttGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTctct                                            <  2:118314/137‑1 (MQ=255)
                                                                                            gAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACt                                       >  5:2546/1‑137 (MQ=255)
                                                                                            gAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACt                                       >  7:19668/1‑137 (MQ=255)
                                                                                            gAAGCGAGGGGCCCGGAGGGGGCGGGTGGCGGTGCTTGAGCAAATTGCCGCTGACCTTGCTTATGGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACt                                       <  2:172820/137‑1 (MQ=255)
                                                                                                      gCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGACTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCa                             >  5:48886/1‑137 (MQ=255)
                                                                                                      gCCCGGATGGGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACAGGCTTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCa                             <  3:241315/137‑1 (MQ=255)
                                                                                                        ccGGAGGTGGCGGTTGGCGGGGCTTTAGCAAATTGCCGCTTAACTTGCTTATGGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAAc                           <  5:2899/137‑1 (MQ=255)
                                                                                                             tgtgGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGAt                      >  7:26660/1‑137 (MQ=255)
                                                                                                             tgggggggttggcggtGATTGAGAAAATTGCCGCTTAACATGCTTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGAt                      <  7:153339/131‑1 (MQ=255)
                                                                                                             ggggggggtgggcggtGCTTGCGCAAATTGCCGCTGAACATGCTTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGAt                      <  8:360676/131‑1 (MQ=255)
                                                                                                             gggggcggtgggcgttGCTTGACAAAATTGCGGCTGAACATGCTTCTCGGGGGGGGGGGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGAt                      <  8:19668/134‑1 (MQ=255)
                                                                                                                  gggttgggggtGCTTGCGAAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                 <  4:267930/136‑1 (MQ=255)
                                                                                                                  gggttggcggtGTTTCACAAAATGGCCGCTTAACATGCTTAGCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                 <  1:203792/136‑1 (MQ=255)
                                                                                                                  gggttggcggtGCTTTAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                 <  2:380539/136‑1 (MQ=255)
                                                                                                                  gggttggcggtGCTTGAGAAAATTGCCGCTTAACTTGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                 <  7:378853/136‑1 (MQ=255)
                                                                                                                  gggttggcggtGCTTGAGAAAATTGCCGCTGAACATGGTTAGGGGGGGGGGGGGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                 <  4:274566/136‑1 (MQ=255)
                                                                                                                  gggtggggggtGCTTTTCCAAATTGCCGCTGAACATGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                 <  8:134344/136‑1 (MQ=255)
                                                                                                                  gggtgggcggtGCTGGCGAAAATTGCCGCTGAACAGGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                 <  2:98779/136‑1 (MQ=255)
                                                                                                                  cggttggcgtgGATTGAGCAAATTCCCGCTTAAATTCCTTTTCGGGTGGGCGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                 <  8:292714/137‑1 (MQ=255)
                                                                                                                  cggttggcggtGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                 >  5:360627/1‑137 (MQ=255)
                                                                                                                    tttggcggtGCTTGAGCAAATGGCCGGTGAACATGCTTTGGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGc               <  8:52444/136‑1 (MQ=255)
                                                                                                                     tgggcggtGCTTGAGCAAATTGCCGCTTAACTTGTTTTTCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGct              <  5:354792/135‑1 (MQ=255)
                                                                                                                         ggttGCTTGAGCAAATTGCCGCTTAACTTGGTTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCAAt          <  5:341438/134‑1 (MQ=255)
                                                                                                                           gtGCTTGAGCAAATTGCCGCTGAACATGATTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGc        <  5:352011/137‑1 (MQ=255)
                                                                                                                           gtGCTTGAGCAAATTCCCGCTCAACATGGTTAGCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGc        <  5:322826/137‑1 (MQ=255)
                                                                                                                                tGATCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGa   <  1:336562/137‑1 (MQ=255)
                                                                                                                                 gAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGAt  >  2:358652/1‑137 (MQ=255)
                                                                                                                                    |                                                                                                                                     
AACGCCACCGCACGTTTCGCCGACCTGGAACTGCGAGTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGAT  >  ACB143/2729989‑2730254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑