Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,730,1490TG41.5% 45.7 / 12.5 53V1336G (GTG→GGG) CJ019_02576hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/13);  new base G (0/22);  total (18/35)
Fisher's exact test for biased strand distribution p-value = 3.74e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GAACTGCGAGTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCTTGAGTAACCAGGACCTTCTCCT  >  ACB143/2730016‑2730284
                                                                                                                                     |                                                                                                                                       
aaccTGCGAGTGATGATATGGCGCACGCGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGGGCCCGGATGTGGCGGTTGGGGTTGCTTGAGAAAATTGCCGCTTAACATGATTATCGgggggg                                                                                                                                      <  6:270668/134‑1 (MQ=255)
         gTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGgcgc                                                                                                                             >  5:137848/1‑137 (MQ=255)
         gTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGgcgc                                                                                                                             >  7:52444/1‑137 (MQ=255)
             tgatATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGaa                                                                                                                         >  4:12922/1‑137 (MQ=255)
                   ggCGCACGCGGCACAGCGGATGTGCCCGGCCTGAGCGGGCCTTGCTGACGCGGGGTGCCGGTATGGGGGGGTGGGCGGTGGTTGAGAAATTTGCCGCTTAACATGGTTTTCGGGTGGGGGCGGGCGCTGAatcgatc                                                                                                                   <  4:321595/137‑1 (MQ=255)
                   ggCGCACGAGGCACCGCGGAGCTGCCCGGCGTGAGGGGGCCTTGCTTAAGCTCGGTGCCCGTTTGGGGGGGTTGGCGGTGCTTTAGAAAATTGCCGCTTAACTTGCTTATGGGGGGGGGGCGGGCGCTGAatcgatc                                                                                                                   <  2:68864/137‑1 (MQ=255)
                         cccggCACCGCGGATCTGCCCGGCGTGAGCGGGCCTTGCTTAAGGTGGGTGCCGGGTTGGGGGGGTTGGCGGTGCTTGAGAAAATTGCGGCTGAACATGGTTATGGGGTGGGGGCGGGCGCTGAATCGATCGAATTc                                                                                                             <  2:57747/134‑1 (MQ=255)
                          gAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCg                                                                                                            >  7:44427/1‑137 (MQ=255)
                          gAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCg                                                                                                            >  3:102270/1‑137 (MQ=255)
                            ggCACAGCGGATCTGCCCGGCCTGCGCGGGCCTTGCTGAAGCTCGGGGCCCGGATGTGGCGGTTGGCGGTGCTTGAGAAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGcc                                                                                                          <  3:2126/137‑1 (MQ=255)
                             gCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGCTGGGGGCGGGCGCTGAATCGATCGAATTCGCCt                                                                                                         >  8:209147/1‑137 (MQ=255)
                                    ggATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGGGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCtt                                                                                                  >  1:376931/1‑136 (MQ=255)
                                      aTCTGCCCGGCGTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGGGGGGGTGGGCGGTGCTTCAGCAAATTCCCGCTGAACATGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATc                                                                                                <  5:277234/137‑1 (MQ=255)
                                         tGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGaa                                                                                             >  7:248821/1‑137 (MQ=255)
                                         tGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGaa                                                                                             >  7:360676/1‑137 (MQ=255)
                                           ccgggCCGGCGCGGGCCTTGGTTAAGCGGGGGGCCGGTTGGGGGGGGTTGGGGGTGCTTTAGAAAATGGCCGCTCAACATCATTAGGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATc                                                                                           <  1:22892/134‑1 (MQ=255)
                                                  tGAGGGGGCCTTGCTGACCCTCGGTGCCCGGATGGGCCGGTTGGCGGTGCTTCAGCAAATTCCGGCTCAACATCCTTATGGGGTGGGGCCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTgc                                                                                    <  8:342914/137‑1 (MQ=255)
                                                  tGAGCGGGCCTTGCTGAAGCTGGGTGCCGGTTTGTGGGGGTTGGGGGTGCTTTACAAATTTGCCGCTTAACATGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTgc                                                                                    <  4:122624/137‑1 (MQ=255)
                                                  tGAGCGGGCCTTGCTGAAGCTGGGTGCCCGGATGTGGCGGTTGGCGGTGCTTTAGAAATTTGCCGCTTAACATGATTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTgc                                                                                    <  3:12922/137‑1 (MQ=255)
                                                  gtaGCGGGCGTTGGTTACGCTGGGTGCCGGTTTTTGGGGGTTGGCGGTGCTTGAGAAAATTGCCGCTTAACATGGTTTGGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTgc                                                                                    <  2:34518/135‑1 (MQ=255)
                                                  ggAGCGGGCCGTGCGTAAGGGCGGGGCCCGGTTGTGGGGGGTGGCGGTGGTTGAGCAAATTGCCGCTGAACAGGCTTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTgc                                                                                    <  8:277766/136‑1 (MQ=255)
                                                   gAGCGGGCCTTGCTGAAGCTCGGTGCCCGGTGGTGGCGGTTGGGGGTGGTTGAGCAAATTGCCGCTTAACATGCTTATGGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTgcg                                                                                   <  3:228197/137‑1 (MQ=255)
                                                            ttGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTctct                                                                          <  2:118314/137‑1 (MQ=255)
                                                                 gAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACt                                                                     >  7:19668/1‑137 (MQ=255)
                                                                 gAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACt                                                                     >  5:2546/1‑137 (MQ=255)
                                                                 gAAGCGAGGGGCCCGGAGGGGGCGGGTGGCGGTGCTTGAGCAAATTGCCGCTGACCTTGCTTATGGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACt                                                                     <  2:172820/137‑1 (MQ=255)
                                                                           gCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGACTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCa                                                           >  5:48886/1‑137 (MQ=255)
                                                                           gCCCGGATGGGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACAGGCTTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCa                                                           <  3:241315/137‑1 (MQ=255)
                                                                             ccGGAGGTGGCGGTTGGCGGGGCTTTAGCAAATTGCCGCTTAACTTGCTTATGGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAAc                                                         <  5:2899/137‑1 (MQ=255)
                                                                                  tgtgGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGAt                                                    >  7:26660/1‑137 (MQ=255)
                                                                                  tgggggggttggcggtGATTGAGAAAATTGCCGCTTAACATGCTTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGAt                                                    <  7:153339/131‑1 (MQ=255)
                                                                                  ggggggggtgggcggtGCTTGCGCAAATTGCCGCTGAACATGCTTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGAt                                                    <  8:360676/131‑1 (MQ=255)
                                                                                  gggggcggtgggcgttGCTTGACAAAATTGCGGCTGAACATGCTTCTCGGGGGGGGGGGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGAt                                                    <  8:19668/134‑1 (MQ=255)
                                                                                       gggttgggggtGCTTGCGAAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                                               <  4:267930/136‑1 (MQ=255)
                                                                                       gggttggcggtGTTTCACAAAATGGCCGCTTAACATGCTTAGCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                                               <  1:203792/136‑1 (MQ=255)
                                                                                       gggttggcggtGCTTTAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                                               <  2:380539/136‑1 (MQ=255)
                                                                                       gggttggcggtGCTTGAGAAAATTGCCGCTTAACTTGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                                               <  7:378853/136‑1 (MQ=255)
                                                                                       gggttggcggtGCTTGAGAAAATTGCCGCTGAACATGGTTAGGGGGGGGGGGGGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                                               <  4:274566/136‑1 (MQ=255)
                                                                                       gggtggggggtGCTTTTCCAAATTGCCGCTGAACATGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                                               <  8:134344/136‑1 (MQ=255)
                                                                                       gggtgggcggtGCTGGCGAAAATTGCCGCTGAACAGGCTTATCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                                               <  2:98779/136‑1 (MQ=255)
                                                                                       cggttggcgtgGATTGAGCAAATTCCCGCTTAAATTCCTTTTCGGGTGGGCGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                                               <  8:292714/137‑1 (MQ=255)
                                                                                       cggttggcggtGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAAt                                               >  5:360627/1‑137 (MQ=255)
                                                                                         tttggcggtGCTTGAGCAAATGGCCGGTGAACATGCTTTGGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGc                                             <  8:52444/136‑1 (MQ=255)
                                                                                          tgggcggtGCTTGAGCAAATTGCCGCTTAACTTGTTTTTCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGct                                            <  5:354792/135‑1 (MQ=255)
                                                                                              ggttGCTTGAGCAAATTGCCGCTTAACTTGGTTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCAAt                                        <  5:341438/134‑1 (MQ=255)
                                                                                                gtGCTTGAGCAAATTGCCGCTGAACATGATTATGGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGc                                      <  5:352011/137‑1 (MQ=255)
                                                                                                gtGCTTGAGCAAATTCCCGCTCAACATGGTTAGCGGGGGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGc                                      <  5:322826/137‑1 (MQ=255)
                                                                                                     tGATCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGa                                 <  1:336562/137‑1 (MQ=255)
                                                                                                      gAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGAt                                >  2:358652/1‑137 (MQ=255)
                                                                                                          aaaTTGCCGCTTAACTTGCTTATGGGGGGGGGGGGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCt                            <  2:217359/137‑1 (MQ=255)
                                                                                                          aaaTTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTCGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCt                            >  6:324896/1‑137 (MQ=255)
                                                                                                                ccGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCCAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCGttt                      >  2:13621/1‑136 (MQ=255)
                                                                                                                         aTGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCTTGAGTAACCAg             >  6:63253/1‑137 (MQ=255)
                                                                                                                                    gTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCTTGAGTAACCAGGACCTTCTCCt  <  8:44427/137‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
GAACTGCGAGTGATGATATGGCGCACGAGGCACAGCGGATCTGCCCGGCCTGAGCGGGCCTTGCTGAAGCTCGGTGCCCGGATGTGGCGGTTGGCGGTGCTTGAGCAAATTGCCGCTGAACATGCTTATCGGGTGGGGGCGGGCGCTGAATCGATCGAATTCGCCTTGGCCTATCGAATCGGCTTGCGCAAGTCTCTGGACTTGCCTGGCCAACCCGATGAAATGCTCTATGCAGGGATCCCTGCCTTGAGTAACCAGGACCTTCTCCT  >  ACB143/2730016‑2730284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑