Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,653,7330TG33.3% 49.9 / 14.9 36V14V (GTA→GTC) mtnB_2Methylthioribulose‑1‑phosphate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/24);  new base G (12/0);  total (12/24)
Fisher's exact test for biased strand distribution p-value = 7.99e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCATGCGGCCCCGGCACGCGGACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGAT  >  ACB143/3653602‑3653861
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tCATGCGGCCCCGGCACGCGGACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGtt                                                                                                                             <  8:10987/137‑1 (MQ=255)
    gCGGCCCCGGCACGCGGACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAg                                                                                                                         <  4:182907/137‑1 (MQ=255)
       gCCCCGGCACGCGGACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTg                                                                                                                      <  4:219111/137‑1 (MQ=255)
             gCACGCGGACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCtggtg                                                                                                                <  3:69278/137‑1 (MQ=255)
             ccACGCGGACCGAGAAGTGCGGGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCtggtg                                                                                                                <  7:185220/136‑1 (MQ=255)
                   ggACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAg                                                                                                          <  1:190935/137‑1 (MQ=255)
                    gACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAgg                                                                                                         <  8:376146/137‑1 (MQ=255)
                    gACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAgg                                                                                                         <  1:56918/137‑1 (MQ=255)
                     aCCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGc                                                                                                        <  2:341662/137‑1 (MQ=255)
                         ataaGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGAc                                                                                                    <  3:167112/135‑1 (MQ=255)
                          gaAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGGCGTTGGAGGTGGTGGGGGTGAAGGCAGACa                                                                                                   >  3:328205/1‑137 (MQ=255)
                                   gtAGATTTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCa                                                                                          <  8:163241/137‑1 (MQ=255)
                                       aTGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATggg                                                                                      <  8:248784/137‑1 (MQ=255)
                                       aTGGGGGCGGTCCAGCGCAGCAGCGCAGACAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATggg                                                                                      <  4:341051/137‑1 (MQ=255)
                                           ggtcggtcCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTGGTTTGAGGGGGGGGTGGTGGAGGGGGGCAGGGTACTCAAGGGGctg                                                                                  >  3:111305/1‑136 (MQ=255)
                                               ggtcCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCg                                                                              <  5:148870/137‑1 (MQ=255)
                                               ggtcCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGGGGTGTCGGGGGGGGGTGGTGGAGGCGGACAGTGAAATGCTTGGGCTCTCCg                                                                              >  3:70918/1‑137 (MQ=255)
                                               ggtcCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGGCGTTGGTGGGGGGGGGGGGGTAGGGAGCCAGTGAGCTCATGGGGCTCGCCg                                                                              >  1:54639/1‑137 (MQ=255)
                                                    agcgcagcagcgcagcCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTCGGTGGAGGTGCTGGGGGTGAAGGCAGACAGTGAACGCCTGGGGCGCTCCgggtat                                                                         >  5:340400/1‑133 (MQ=255)
                                                             gcgcagcCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTCGGTGGCGGGGGTGGGGGTGGAGGGGGACAGTGTACTGATTGGGCTCTCCGGGTATAAATATccc                                                                >  5:282780/1‑136 (MQ=255)
                                                              cgcagcCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGGCGGTGGCGGGGGGGGGGGGGGTGGCGGACACGGAACACATGGGGCTCTCCGGGAATCAAAAgaccc                                                               >  3:164834/1‑133 (MQ=255)
                                                              cgcagcCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGACGTTGGGGGGGGGGGTGGTGTAGGCAGCCAGTGATCTCATGGGAGTCTCCGGGAATAAATATCcgc                                                               >  5:140320/1‑137 (MQ=255)
                                                                      gACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTCGTTTGGGGTGGGGGGGGGGTGGGGGGCCACTGAACTTATGGGGCACGGCGGGTATCAAAAGCCGCGCCCCggg                                                       >  2:306634/1‑135 (MQ=255)
                                                                       aCGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCgtgt                                                      <  1:247752/137‑1 (MQ=255)
                                                                            aaGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGAcc                                                 <  2:31733/137‑1 (MQ=255)
                                                                            aaGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGGCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGAcc                                                 <  4:96545/137‑1 (MQ=255)
                                                                                  cggcCAGCGTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGGCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTg                                           <  1:163381/137‑1 (MQ=255)
                                                                                       aGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTCGGTGGAGGGGGGGGGGGGGTAGGCAGACAGTGAACTCATGGGGGTCGCCGGGAATAAAAAGCCGCGCGCCGGGTCGCCCAGGGTGGCCac                                      >  4:131801/1‑136 (MQ=255)
                                                                                                     cTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTcgc                        <  6:140320/137‑1 (MQ=255)
                                                                                                     cTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTcgc                        <  4:170196/137‑1 (MQ=255)
                                                                                                     cTCCCACTCTTCGGCGCTGACCCGGTCGCGGGGGGTTGGGGGGGGGGGGGGGTAGGGAGACAATGGACGAAAGGGTCTCTGCGGGTATAAATATCCCCGCGCCGGGTCGACCACGGTGGGGAGGGGGGCCTGGTcgg                        >  6:320257/1‑136 (MQ=255)
                                                                                                           ctctTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGTCTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGc                  <  6:173492/137‑1 (MQ=255)
                                                                                                            tctTCGGCGCTGACCCGGTCGCGGTCGGGGGTGGGGGGGGTGGGGGAGACACACAGGGCACTCATGGGGCTCTGCCGGAATAAATAGCCCCCCGCCCGGTCGCCCCCGGTGGGCAGGGGGGCCCGCGCGCgggggcg                 >  4:259941/1‑132 (MQ=255)
                                                                                                                 ggCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGa            <  6:204893/137‑1 (MQ=255)
                                                                                                                         cccGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCg    <  2:233593/137‑1 (MQ=255)
                                                                                                                           cGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGAt  <  4:164834/137‑1 (MQ=255)
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TCATGCGGCCCCGGCACGCGGACCGAGAAGTGCGTGTAGATGTGGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGAT  >  ACB143/3653602‑3653861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑