Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,653,7780AG23.1% 78.8 / 10.0 41intergenic (‑4/+12)mtnB_2/CJ019_03463Methylthioribulose‑1‑phosphate dehydratase/Hydroxypyruvate reductase
Reads supporting (aligned to +/- strand):  ref base A (8/22);  new base G (9/0);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 1.15e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGGGTAACCGTCCTCGGAAATGGCCGAGGTGTGC  >  ACB143/3653645‑3653913
                                                                                                                                     |                                                                                                                                       
ggtcggtcCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTGGTTTGAGGGGGGGGTGGTGGAGGGGGGCAGGGTACTCAAGGGGctg                                                                                                                                      >  3:111305/1‑136 (MQ=255)
    ggtcCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCg                                                                                                                                  <  5:148870/137‑1 (MQ=255)
    ggtcCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGGGGTGTCGGGGGGGGGTGGTGGAGGCGGACAGTGAAATGCTTGGGCTCTCCg                                                                                                                                  >  3:70918/1‑137 (MQ=255)
    ggtcCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGGCGTTGGTGGGGGGGGGGGGGTAGGGAGCCAGTGAGCTCATGGGGCTCGCCg                                                                                                                                  >  1:54639/1‑137 (MQ=255)
         agcgcagcagcgcagcCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTCGGTGGAGGTGCTGGGGGTGAAGGCAGACAGTGAACGCCTGGGGCGCTCCgggtat                                                                                                                             >  5:340400/1‑133 (MQ=255)
                  gcgcagcCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTCGGTGGCGGGGGTGGGGGTGGAGGGGGACAGTGTACTGATTGGGCTCTCCGGGTATAAATATccc                                                                                                                    >  5:282780/1‑136 (MQ=255)
                   cgcagcCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGGCGGTGGCGGGGGGGGGGGGGGTGGCGGACACGGAACACATGGGGCTCTCCGGGAATCAAAAgaccc                                                                                                                   >  3:164834/1‑133 (MQ=255)
                   cgcagcCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGACGTTGGGGGGGGGGGTGGTGTAGGCAGCCAGTGATCTCATGGGAGTCTCCGGGAATAAATATCcgc                                                                                                                   >  5:140320/1‑137 (MQ=255)
                           gACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTCGTTTGGGGTGGGGGGGGGGTGGGGGGCCACTGAACTTATGGGGCACGGCGGGTATCAAAAGCCGCGCCCCggg                                                                                                           >  2:306634/1‑135 (MQ=255)
                            aCGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCgtgt                                                                                                          <  1:247752/137‑1 (MQ=255)
                                 aaGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGAcc                                                                                                     <  2:31733/137‑1 (MQ=255)
                                 aaGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGGCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGAcc                                                                                                     <  4:96545/137‑1 (MQ=255)
                                       cggcCAGCGTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGGCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTg                                                                                               <  1:163381/137‑1 (MQ=255)
                                            aGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGGTCGGTGGAGGGGGGGGGGGGGTAGGCAGACAGTGAACTCATGGGGGTCGCCGGGAATAAAAAGCCGCGCGCCGGGTCGCCCAGGGTGGCCac                                                                                          >  4:131801/1‑136 (MQ=255)
                                                          cTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTcgc                                                                            <  6:140320/137‑1 (MQ=255)
                                                          cTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTcgc                                                                            <  4:170196/137‑1 (MQ=255)
                                                          cTCCCACTCTTCGGCGCTGACCCGGTCGCGGGGGGTTGGGGGGGGGGGGGGGTAGGGAGACAATGGACGAAAGGGTCTCTGCGGGTATAAATATCCCCGCGCCGGGTCGACCACGGTGGGGAGGGGGGCCTGGTcgg                                                                            >  6:320257/1‑136 (MQ=255)
                                                                ctctTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGTCTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGc                                                                      <  6:173492/137‑1 (MQ=255)
                                                                 tctTCGGCGCTGACCCGGTCGCGGTCGGGGGTGGGGGGGGTGGGGGAGACACACAGGGCACTCATGGGGCTCTGCCGGAATAAATAGCCCCCCGCCCGGTCGCCCCCGGTGGGCAGGGGGGCCCGCGCGCgggggcg                                                                     >  4:259941/1‑132 (MQ=255)
                                                                      ggCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGa                                                                <  6:204893/137‑1 (MQ=255)
                                                                              cccGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCg                                                        <  2:233593/137‑1 (MQ=255)
                                                                                cGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGAt                                                      <  4:164834/137‑1 (MQ=255)
                                                                                          cGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCg                                            <  4:315927/137‑1 (MQ=255)
                                                                                            ttGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCgag                                          <  4:328205/137‑1 (MQ=255)
                                                                                                aGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAg                                      >  5:111421/1‑137 (MQ=255)
                                                                                                     cTGGTGGTGAAGGCAGACAGTGACCTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCgg                                 <  5:96138/137‑1 (MQ=255)
                                                                                                          ggtgAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATa                                                                                                                        >  4:365101/1‑45 (MQ=255)
                                                                                                          ggtgAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATa                                                                                                                        <  3:365101/45‑1 (MQ=255)
                                                                                                                     aCAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGGGTAACCGTACTCGGaa                 >  1:279409/1‑137 (MQ=255)
                                                                                                                        gTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAg                                                              >  7:119096/1‑89 (MQ=255)
                                                                                                                        gTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAg                                                              <  8:119096/89‑1 (MQ=255)
                                                                                                                         tGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGGGTAACCGTCCTCGGAAATgg             <  4:70918/137‑1 (MQ=255)
                                                                                                                           aaCTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGGGTAACCGTCCTCGGAAATGGcc           <  4:111305/137‑1 (MQ=255)
                                                                                                                              tCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGGGTAACCGTCCTCGGAAATGGCCGAg        >  5:328507/1‑137 (MQ=255)
                                                                                                                                aTGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGGGTAACCGTCCTCGGAAATGGCCGAGgt      <  4:292387/137‑1 (MQ=255)
                                                                                                                                aTGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGGGTAACCGTCCTCGGAAATGGCCGAGgt      <  1:18291/137‑1 (MQ=255)
                                                                                                                                  gggACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGGGTAACCGTCCTCGGAAATGGCCGAGgtgt    >  3:89993/1‑137 (MQ=255)
                                                                                                                                   ggACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGGGTAACCGTCCTCGGAAATGGCCGAGGtgtg   >  7:83572/1‑137 (MQ=255)
                                                                                                                                    gACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGGGTAACCGTCCTCGGAAATGGCCGAGGTGTGc  <  5:320257/137‑1 (MQ=255)
                                                                                                                                    gACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGGGTAACCGTCCTCGGAAATGGCCGAGGTGTGc  <  4:344570/137‑1 (MQ=255)
                                                                                                                                    gACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGGGTAACCGTCCTCGGAAATGGCCGAGGTGTGc  <  4:338584/137‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
GGTCGGTCCAGCGCAGCAGCGCAGCCAGACGGTAAGCGGCGGCCAGCTTGACCCGCACCTCCCACTCTTCGGCGCTGACCCGGTCGCGTACGTTGCAGGTGCTGGTGGTGAAGGCAGACAGTGAACTCATGGGACTCTCCGGTAATCAATAGCCGCGCGCCGTGTCGACCAGGTTGGCCAGGGGCGCCTGCTCGCGGTAGCGGTGGAAGTTGGCGATGAAGGCATCGAGAAAGGCCGGGTAACCGTCCTCGGAAATGGCCGAGGTGTGC  >  ACB143/3653645‑3653913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑