Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,784,7670TG41.3% 49.0 / 11.0 47S531R (AGC→CGC) dmdC_43‑methylmercaptopropionyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (18/9);  new base G (1/18);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 3.90e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGATGGCC‑GCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCGTGTCGCACGC  >  ACB143/3784647‑3784902
                                                                                                                         |                                                                                                                                       
ccgtGGCCGGCCAGCGGCGGGTCGTGGGGGGGCCGAATGAAGCCGCGTACGAACCCACGGCTTGCCTCGTTTTCCCGGCGCTGCGCCGCCCCCACTGCCACCGCGTGCTTCCCGCGCGGGCGGCGGGCCCAGGCGaa                                                                                                                          <  6:188928/134‑1 (MQ=255)
           cACCGGCCGGCCGTGGGCGGGCAGCCGGACCCCGAGCAAGACCCCAACGCTCTCCTGCTTTTCCCGGCGCGGCGCCGCCCCCACTGCCACCGCCTGGATGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTgcagca                                                                                                               <  5:254359/137‑1 (MQ=255)
              gtGCCGGTCGCGGTCGGGCAGCAGGTAGCCGAGCAACAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCg                                                                                                            <  7:136005/136‑1 (MQ=255)
                   ggTCGAGGGCGGGCAGCAGGTAGCCGAGTAAGACCCCAACGCTTTCCTGCTTTGCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTgcg                                                                                                       <  6:73753/137‑1 (MQ=255)
                    gTCGTGGGCGGGCAGCAGGTCCCCGAGGAACACCCCACCGATTTCCTGCTTTGCCCGGCGCTGCGCCGCCCCCACTGCCACCGCCTGGATGCAGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTgcgc                                                                                                      <  7:199467/137‑1 (MQ=255)
                           tCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTg                                                                                               >  3:282603/1‑137 (MQ=255)
                            cGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGc                                                                                              >  8:122865/1‑137 (MQ=255)
                                      gTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGc                                                                                    >  3:254270/1‑137 (MQ=255)
                                           ggccgaacaaCACACCGCTTGCCTGCTTTGCCCGGCGCTCGCCCGCCCCCCCTGGCAACGCCGGGAGGCACCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTgcg                                                                               <  2:360619/133‑1 (MQ=255)
                                           cgcgaaagaaCCTAACGCTTGCGTGCTTTTCCCGGCGCTGCGCCGCCCCCCCTGCCACCGCCTGGAGGCCCCGCGGGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTgcg                                                                               <  7:196582/132‑1 (MQ=255)
                                                  gaaCCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCggg                                                                        >  3:109729/1‑136 (MQ=255)
                                                           gCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAAtt                                                               >  6:200283/1‑137 (MQ=255)
                                                            atttCCTGCTTTGCCCGGCGCGGCGCCGCCCCCACTGGCAACGCCTGGAGGCCGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTc                                                              <  1:121892/136‑1 (MQ=255)
                                                              ttCCGGCTTTTCCCGGCGCGGGGCCGCCCCCACTGCCAACGCCTGGAGGCAGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCgg                                                            <  5:5156/137‑1 (MQ=255)
                                                                  tGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCCGCACCGTGGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGAta                                                        >  4:262561/1‑136 (MQ=255)
                                                                  tGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATc                                                        >  5:260507/1‑137 (MQ=255)
                                                                  tGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAAATCGGGATc                                                        >  7:272464/1‑137 (MQ=255)
                                                                  tGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCCTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGGACGGAAATTCGGGAta                                                        >  3:175743/1‑136 (MQ=255)
                                                                  tGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCAGCGGGCCCAGGCGAAGGCCTGCAGAACCGTTGCGCACAACTGCAGGAAGTCGCATGCGGCTCGGTTCGGAAATTCGGGAtg                                                        >  8:307179/1‑136 (MQ=255)
                                                                    cTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGTATGCCGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGc                                                      <  3:42215/137‑1 (MQ=255)
                                                                    attatCCCGGCGCTCCGCCGCCCCCACTCTCAACGCCGGGATGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCTCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGc                                                      <  4:171304/133‑1 (MQ=255)
                                                                         cccGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCCGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCcgcg                                                 <  4:109729/137‑1 (MQ=255)
                                                                         cccGGCGCGGCGCCGCCCCCACTGCCAACGCCTGGCCGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCcgcg                                                 <  7:275087/137‑1 (MQ=255)
                                                                            ggCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTc                                              >  3:360811/1‑137 (MQ=255)
                                                                            ggCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGAAGCGCGCGCGGCGGGCCCAGGCGACGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGACTGCGGCGCGGTACGGAAATTCGGGATAGCCCGCTGtg                                              >  8:306498/1‑136 (MQ=255)
                                                                                cTGCGCCGCCCCCACTGGCCACGCCTGGATCCAGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCg                                          <  8:347346/137‑1 (MQ=255)
                                                                                cTGCGCCGCCCCCACTGGCAACGCCGGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCg                                          <  7:290197/137‑1 (MQ=255)
                                                                                cTGCGCCGCCCCCACTGCCATCGCGTGGCGGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCg                                          <  1:180438/137‑1 (MQ=255)
                                                                                          cccACGGGCACCGCGGGGATGCCGCGGGGGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCAGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCtt                                <  1:187658/137‑1 (MQ=255)
                                                                                           ccACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTg                               >  6:290156/1‑137 (MQ=255)
                                                                                               tGGCACCGCCTGGATGCCGCGCGCGGGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCa                           <  3:47734/137‑1 (MQ=255)
                                                                                               tGGCACCGCCTGGATGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCa                           <  2:72098/137‑1 (MQ=255)
                                                                                               ggccaccGCCGGCATGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCa                           <  1:272993/131‑1 (MQ=255)
                                                                                                 gCAACGCCTGGATGCAGCGCGGGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAgc                         <  6:260507/137‑1 (MQ=255)
                                                                                                 ccAACGCCTGGATCCAGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAgc                         <  4:128042/136‑1 (MQ=255)
                                                                                                    aCGCCTGGATGCCGCGGGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCt                      <  5:184277/137‑1 (MQ=255)
                                                                                                        cTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACCACTGCAGGAAGTCGCCTGCGGCGCCGTAAGGAAATTCGGGATAGCCCGAGGTCAACGACAGCACCATGGCCAGCGCTGcca                  >  8:306629/1‑136 (MQ=255)
                                                                                                        cTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCt                  >  7:222872/1‑137 (MQ=255)
                                                                                                         tGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTc                 >  4:355273/1‑137 (MQ=255)
                                                                                                          ggATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGGCATCGACAGCCCCTTGGCCAGCGCTGCCTcc                >  3:24638/1‑137 (MQ=255)
                                                                                                            agcccgcgcgcgcGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAg              <  5:210108/132‑1 (MQ=255)
                                                                                                            aTGCAGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAg              <  7:122865/137‑1 (MQ=255)
                                                                                                                 gcgcgcgcTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCgtgt         <  7:303807/137‑1 (MQ=255)
                                                                                                                 gcgcgcgcTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCgtgt         <  1:126700/137‑1 (MQ=255)
                                                                                                                 ccgcgcgcgGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCgtgt         <  3:184968/136‑1 (MQ=255)
                                                                                                                     gcgcTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCGTGTcgcc     >  7:36742/1‑136 (MQ=255)
                                                                                                                     gcgcTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGGCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCGTGTcgcc     >  1:265454/1‑136 (MQ=255)
                                                                                                                        cTGCGGGCCCAGGCGAAGGACTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGGGGCGCGGTACGGAAATTCGGGATGGCCCGCGGTCATGGACAGCCCCTTGGCCAGCGCTGGCCCCAGCGTGTcgaccgc  >  4:207614/1‑132 (MQ=255)
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CGATGGCC‑GCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCGTGTCGCACGC  >  ACB143/3784647‑3784902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑