Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,620,1580GC29.3% 39.5 / 13.8 42C265W (TGC→TGG) CJ019_05281hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (11/18);  new base C (0/12);  total (12/30)
Fisher's exact test for biased strand distribution p-value = 1.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGTGCTCGGCCTCTATCTCACCGGCG  >  ACB143/5620028‑5620293
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ccGGTAACGGGAAGGGGTTTAAGTGGCGGAACACGCGGATATCGGCCTGGTGGGCTTCACGTGAGGCGGCCGGGTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGC‑TGTATGCAAcgc                                                                                                                                   <  8:172275/137‑1 (MQ=255)
      acgggaacgggTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCGGCATGGCAGGCAGCAGCAGGCAAAAGTAGCCCACC‑TGTATGCAACGCGACAcc                                                                                                                             >  2:233204/1‑137 (MQ=255)
          taacgggGTCAACTGGCGGAACACGCGTATAGCGGCCCGGTCGGCTCCACGTGAGGCGGCCGGCGGGTCGGGTGCGGCCGGCATGGCAGGCAGCTGCAGGCAAAAGACCCCCAGC‑TGTATCCAACGCGACTCCGGCa                                                                                                                         <  6:5877/136‑1 (MQ=255)
          caacgggTTGAACTGGCGCAACACGCGGATATCGGCCAGGTCGGCTTCCCGGGAGGCGGCCGGCTGGTCGGGGGCTGCCTGCATGGCAGGCAGGTGCAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCGGCa                                                                                                                         <  6:334105/136‑1 (MQ=255)
                 ttGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTTCCAGCCTGGCACGCAGCTGCAGGCAAAAGTATCCCAGC‑TGTATGCAACGCGACTCCGGCACCCCGGc                                                                                                                  >  2:97043/1‑137 (MQ=255)
                    acggggCGCAACCCGCGGTTAGCGGCCTGGGGGGGTCCCCGTGCGGCGGCGGGGGGGGGGGGGGCGGCCTGCAGGGCTGGCAGCTGCAGGCAAAAGACCCCCCGC‑TGTATCCACCGCGACTCCGGCACGCCGGCCCa                                                                                                               <  2:165665/133‑1 (MQ=255)
                     aCTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCAGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTACGGCACGCCCGCCCaa                                                                                                              >  6:89421/1‑137 (MQ=255)
                            gAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCGGCATGGCAGGCACCTGCCGGCAAAAGTACCCCATC‑TGTAAGCCACGCGACTCCCGCCCGCCGGGCCAACGCCGCa                                                                                                       >  8:97006/1‑137 (MQ=255)
                                 cgcgGATATCGGCCAGGTCGGCTTCACGTGTGGCGGCGGGCGGGGCGGGTGCTGCCTGCATGGCAGGAAGCTGCAGGCAAAATTAGCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCacgacg                                                                                                  <  8:276633/137‑1 (MQ=255)
                                    gtatagCGCCCAGGGCGGCTTCCCGGGCGGCGGCCGGCTGGTCGGGTGCGGCCTGCATGGCAGGCAGCGGACGGCAAAATTCCCCCCGC‑GGAATCCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATg                                                                                               <  4:251141/135‑1 (MQ=255)
                                                   cGGCTTCACGTGAGGAGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCACGCAAAAGTAGCCCCGA‑TGGATGCAACGAGAATCCGGAAAGCCGGCCCAACGCGGCACCAACATGCCGTCCCACGCAAGc                                                                                >  8:71704/1‑137 (MQ=255)
                                                    ggCTTCAGGTCCGGCGGCCGGCTGGGGGGGTGCTGCCTGCAGGCCAGCCAGCGGCAGCCAAAAGTCCCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCa                                                                               <  8:53165/137‑1 (MQ=255)
                                                    ggCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCCGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCa                                                                               <  2:259339/137‑1 (MQ=255)
                                                                   ggccggcTGGTCGGGTGCTGCCTGCATGGCACGCAGCAGCAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCCGCACGCCGGCCCAACGCGGCCCGACGATGCTGTCCCACGCACGCAGGCGCCCCATAAGgc                                                                >  6:180059/1‑137 (MQ=255)
                                                                   ggccggcTGGGCGGGTGCGGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGgc                                                                <  6:128353/137‑1 (MQ=255)
                                                                   gcccggcGGGGCGGGGGCGGCCTGCCTGGCAGGCAGCGGCAGCCAAAAGTAGCCCCGC‑TGTATCAAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGgc                                                                <  2:354609/135‑1 (MQ=255)
                                                                       ggcTGGTCGGGTGCTGCCTGGATGGCAGGCAGCTGCAGGCAAAAGTAGCCCCGC‑TGTATGCAACGCGCCGCCGGCACGCCGGCCCCACGCGGCCCGCCGCTGGTGTCCCAGGCCCGCCGGCGCCCCGTCCGgcgcgc                                                            >  2:53451/1‑137 (MQ=255)
                                                                       ggcTGGTCGGGTGCTGCCGGCATGGGAGGCAGCGGCAGGGAAAAGTATCCCCCCGTGT‑TGCAACCCGCCACCCGCCCGCCCGCCCCACCCGGCCCCACGATGCTGTGCCCGGCCCCCCGGCGCCCCATCCGgcgcgc                                                            >  2:284875/1‑137 (MQ=255)
                                                                         gggggcGGGGGCGGCCGGCAGGCCCGGCAGCTGCCGCCAAAAGCCCCCCCGC‑TGTAGCCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcgg                                                          <  1:119577/132‑1 (MQ=255)
                                                                              ggggTGCTGCCTGCATGGAAGGCAGCTGCAGGAAAAAGTAGCCCACC‑TGTATGAACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcggcctcg                                                     <  8:50568/136‑1 (MQ=255)
                                                                              ggggTGCGGCCGGCATGCCTGGCAGCTCCAGCCAAAAGTCCCCCCGC‑TGTAGCCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcggcctcg                                                     <  1:310375/136‑1 (MQ=255)
                                                                                   gcggcctgcAGGCCAGCCCGGTGCAGCCAAAAGACCCCCCGC‑TTTAGCCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCtt                                                <  3:20152/134‑1 (MQ=255)
                                                                                    ctgcctgcTTGGCAGGCTGCTGCAGCCAAAAGTACCCCCCC‑TGTAGCCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCttt                                               <  2:26649/137‑1 (MQ=255)
                                                                                         ggcAGGGCAGCAAGCTGCCGGAAAAAGTCGCCCCGT‑TGTATGCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAg                                          <  4:3818/136‑1 (MQ=255)
                                                                                          gcATGGCAGGCAGCGGCAGGCAAAAGTAGCCCAGC‑TGTATTCAAACCGCCTACCGCCCCCCGGCCCAACCCCGCACCCCCAATCTGTACCCGGCCCGCACGCCCCCCATAAGGCGCGCGGCCACCGCCATTTGCAgg                                         >  2:223138/1‑137 (MQ=255)
                                                                                           cagggcaggcaGCGGCAGCCAAAAGTACCCCAGC‑TGTATCCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGc                                        <  3:115629/134‑1 (MQ=255)
                                                                                           aaTGGCAGGCAGCGGCAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGc                                        <  8:57646/136‑1 (MQ=255)
                                                                                              ggcaggcaGCTGCAGGAAAAAGTACCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCa                                     <  5:77842/137‑1 (MQ=255)
                                                                                               gcaggcagCGGCAGGCAAAAGAAGCCCAGC‑TGTATGCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCAt                                    <  7:46999/137‑1 (MQ=255)
                                                                                                caggctgCTGCCGGCAAACGTAGCCCAGC‑GGTTTGCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATc                                   <  1:227700/137‑1 (MQ=255)
                                                                                                   gcagCGGCAGCAAAATGTCCCCCCGC‑TTTATCAACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATctgc                                <  3:321381/137‑1 (MQ=255)
                                                                                                      gctgacggCAAATGTAGCCGAGC‑GGAAGCCAACCCGACGCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTgg                             <  6:345872/131‑1 (MQ=255)
                                                                                                      gCTGCAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTgg                             >  7:118922/1‑137 (MQ=255)
                                                                                                          cagtaaaaaGTACCCCACC‑TGAATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGt                         <  7:71709/132‑1 (MQ=255)
                                                                                                          cAGGCAAACGTAGCCCAGC‑TGTATGCACCGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGt                         <  7:71704/137‑1 (MQ=255)
                                                                                                          cAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGt                         <  5:180059/137‑1 (MQ=255)
                                                                                                          cAGGCAAAAGTAGCCCAGC‑TGTATCCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGt                         <  5:383171/137‑1 (MQ=255)
                                                                                                                                tATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGTGCTCGGCCTCTATCTCACcgg    >  7:264148/1‑137 (MQ=255)
                                                                                                                                tATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGTGCTCGGCCTCTATCTCACcgg    >  5:383844/1‑137 (MQ=255)
                                                                                                                                 aTGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGTGCTCGGCCTCTATCTCACcggc   <  2:359998/137‑1 (MQ=255)
                                                                                                                                  tGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGTGCTCGGCCTCTATCTCACcggcg  <  4:320074/137‑1 (MQ=255)
                                                                                                                                  tGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGTGCTCGGCCTCTATCTCACcggcg  <  4:314300/137‑1 (MQ=255)
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CCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGC‑TGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAGTGCTCGGCCTCTATCTCACCGGCG  >  ACB143/5620028‑5620293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑