| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 5,386,510 | 2 bp→TA | intergenic (‑519/+1131) | CJ019_05070 ← / ← nptII | hypothetical protein/neomycin phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,386,510 | 0 | A | T | 90.9% | 52.1 / ‑0.9 | 22 | intergenic (‑519/+1132) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (12/8); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 | |||||||||||
| * | ACB143 | 5,386,511 | 0 | T | A | 95.5% | 60.6 / ‑3.1 | 22 | intergenic (‑520/+1131) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (12/9); minor base C (1/0); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01 | |||||||||||
CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑CA‑‑GGA‑TC‑‑AT‑‑C‑CGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCT > ACB143/5386410‑5386607 ||| gcctttttATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGcc < 5:75039/134‑1 (MQ=255) tttttATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGccccc < 2:21790/137‑1 (MQ=255) aaaCAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCAc < 2:8392/136‑1 (MQ=255) cGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttt > 4:361102/1‑137 (MQ=255) gAATACTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTGGGCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCtttt < 7:245488/137‑1 (MQ=255) ttttCATTTAACACCCTAATATCACATCTCGCCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGT‑‑‑GAACGGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt < 1:170691/136‑1 (MQ=255) aTTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCt < 1:325080/137‑1 (MQ=255) tttAATACCGTAATATGACATCTCGGGAGGCTTGTCGACGACGGCGGTCTCAGTGGTGT‑‑‑CA‑‑GGT‑AC‑‑ATACC‑CCGACGCTAGCAAAGCATGAGAAAGCCACCGGAATATCTCGTTCCGGGGGCTTTTTTATTGCGCTg < 8:4602/137‑1 (MQ=255) aaTATGACATCTCGGCCGGCTTTGCGACGACGGGGGTCTCAGTGGGGTA‑‑CT‑‑GTACAA‑‑AC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 5:360596/137‑1 (MQ=255) atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAggg > 8:161605/1‑137 (MQ=255) tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc > 4:296994/1‑137 (MQ=255) tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc > 6:388511/1‑137 (MQ=255) atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCg > 5:155029/1‑137 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTTCGATGCGGGTGCCAGGGCGTGCCCtt > 6:156043/1‑137 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt > 8:189773/1‑137 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGAACACCTTCCGGGGGCGTTTTTATTTGGCAGCGGGTGCCAGGGCGGGCCCtt > 1:194952/1‑137 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTAAGT‑‑GGTGTACG‑‑GTA‑CA‑‑AA‑‑CACCTACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt > 6:374407/1‑137 (MQ=255) ctcGGCCGGCTTGGCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt < 5:355881/137‑1 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCCCCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg > 5:148875/1‑137 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg > 1:305794/1‑137 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg > 1:117363/1‑137 (MQ=255) ccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGT‑ACAAAC‑‑C‑CCGACGCTAGCAACGCATGAGAAAGCCCCAGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCt > 1:243328/1‑137 (MQ=255) ||| CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑CA‑‑GGA‑TC‑‑AT‑‑C‑CGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCT > ACB143/5386410‑5386607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |