Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB143 | 3,181,018 | G→A | 100% | A532T (GCC→ACC) | actP_2 → | Cation/acetate symporter ActP |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB143 | 3,181,018 | 0 | G | A | 100.0% | 164.7 / ‑6.8 | 60 | A532T (GCC→ACC) | actP_2 | Cation/acetate symporter ActP |
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (37/20); minor base C (3/0); total (40/20) | |||||||||||
Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TGTTGCACTTTGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGGCCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAGTAACCTCTG > ACB143/3180890‑3181146 | tgtTGCACTTTGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGc > 1:400870/1‑136 (MQ=255) gtTGCACTTTGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGGTCTCCATGCCGCTGGCGTTTTTGGTGGCGTAGGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGcc < 1:158938/136‑1 (MQ=255) cACTTTGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCACGGCCGAAAGGGCGACCTTCGCCGAcc > 6:19949/1‑136 (MQ=255) cACTTTGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGAc > 5:25225/1‑135 (MQ=255) aCTTTGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTGCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCa < 4:58390/136‑1 (MQ=255) tGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTc > 1:229166/1‑136 (MQ=255) tGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCCGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGCCCATCGCCCACCAGGTc > 6:52049/1‑136 (MQ=255) cGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCCCTGCGCGGGCCAAGGCCGAAAGGGCGCCCTTCGCCGACCAGTTCGTc > 8:80602/1‑136 (MQ=255) cGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAACGGGAGACCTTCGCCGACCAGTTCGTc > 7:382523/1‑136 (MQ=255) tGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCa < 8:267723/136‑1 (MQ=255) ttCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCa < 6:16228/134‑1 (MQ=255) cccTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCg < 4:385208/136‑1 (MQ=255) cTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGc < 8:112928/136‑1 (MQ=255) cTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGTTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCTTCCGTGGCCAGACCTGCCTTGGTGCCAg > 2:97987/1‑136 (MQ=255) cTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCCAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTTGCCCGACCCGACTTGGTGCCAg > 2:153084/1‑136 (MQ=255) cTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGCCCTTCGCCGACCAGTTCGTCCGGGGCCAGACCGGCCATGGTGcccg > 4:278648/1‑134 (MQ=255) cTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGCCCGGCCTTGGTGcccg > 6:460/1‑134 (MQ=255) cTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAg > 5:63758/1‑136 (MQ=255) cTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAg > 2:308615/1‑136 (MQ=255) cTGCGCTGTTATCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTACCCCCATGGACCGCCATGCGCGGGCAAAGGCCGAAAGCGCGACCTTCGCCGACCAGTTAGTCCGAGCACAGACCGGCCTTGGTGCCAg > 5:393491/1‑136 (MQ=255) gcgcTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACAAGTTCGTCCGTGGCCAGACCGGCCTTGGTTCCcgcg > 5:209504/1‑136 (MQ=255) tGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGCCCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGc > 7:370853/1‑136 (MQ=255) tGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGc > 3:263868/1‑136 (MQ=255) tGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGc > 1:260077/1‑136 (MQ=255) ttCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGTGTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGc < 6:218472/134‑1 (MQ=255) tcCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGCGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTc > 1:321216/1‑135 (MQ=255) cATGCGGCTGGAGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGa < 3:278648/135‑1 (MQ=255) gCTGGCGTTTTTGGTGGCGTATGCGGTTTGCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGTGACCTTCGCCGACCAGTTAGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCAtt > 4:401723/1‑134 (MQ=255) tGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGt > 7:365024/1‑136 (MQ=255) tGGCGTTTTTGGTGGCGTATGCGGTTTCACGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGCCCAGTTCGTCCGTGGCCAGCCCGGCCTTGGTGCCAGCGGTGCCGTCGATAATTGAGt > 8:136660/1‑136 (MQ=255) cGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTAc > 3:358425/1‑136 (MQ=255) tggtggCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCcgcg < 2:60525/135‑1 (MQ=255) gtgtCGTATGCGGTGTCCCGGATGCCCCGCACTGCGCGGGCCAAGGCCGAATGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGGGCCGGCGATCATTGAGTTCCCCGCGGc < 4:248464/135‑1 (MQ=255) gCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCa < 4:263868/136‑1 (MQ=255) gtctGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAg < 5:110687/133‑1 (MQ=255) tGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAgcg < 1:308615/135‑1 (MQ=255) ccGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGcc < 6:172361/136‑1 (MQ=255) cGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGc > 3:127996/1‑134 (MQ=255) ggATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCaa > 3:318045/1‑135 (MQ=255) aTGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCg < 3:368594/136‑1 (MQ=255) tGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGa > 5:258511/1‑134 (MQ=255) gccgcgGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTTAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATa < 7:139623/134‑1 (MQ=255) cgcgGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATa < 3:361450/134‑1 (MQ=255) gggCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCg > 1:65025/1‑136 (MQ=255) gggCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCg > 6:279863/1‑136 (MQ=255) gggCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCg > 4:333811/1‑136 (MQ=255) gggCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGATCg > 7:365996/1‑136 (MQ=255) gCCAAGGCCTAAAGGGCGACCTTCGCCGACCAGTTCTTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCTATAATTTAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCt < 5:52049/136‑1 (MQ=255) gCCAAGGCCGAAAGGGCGACCTTCGCCTACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTAGCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCt < 7:54951/136‑1 (MQ=255) gCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCt < 7:292994/136‑1 (MQ=255) aaGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAg > 4:296008/1‑135 (MQ=255) cGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAGTAACct > 6:384001/1‑136 (MQ=255) cGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAGTAACct > 8:190950/1‑136 (MQ=255) gggCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAGTAACCTCTg > 2:75526/1‑134 (MQ=255) gggCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAGTAACCTCTg > 5:163407/1‑134 (MQ=255) gggCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAGTAACCTCTg > 2:292822/1‑134 (MQ=255) gggCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAGTAACCTCTg > 2:17752/1‑134 (MQ=255) gggCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAGTAACCTCTg > 8:360529/1‑134 (MQ=255) gggCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAGTAACCTCTg > 3:144574/1‑134 (MQ=255) gggCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTAGCTAGTAACCTCTg > 3:376623/1‑134 (MQ=255) | TGTTGCACTTTGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGGCCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAGTAACCTCTG > ACB143/3180890‑3181146 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |