Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 445,359 A→G 73.8% H76R (CAC→CGC)  argD_1 → Acetylornithine aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143445,3590AG73.8% 149.4 / 52.1 81H76R (CAC→CGC) argD_1Acetylornithine aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (12/9);  new base G (30/29);  total (42/39)
Fisher's exact test for biased strand distribution p-value = 8.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01

CCTGAGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGTGACCAGGCC  >  ACB143/445226‑445492
                                                                                                                                     |                                                                                                                                     
ccTGAGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACCGCTTGGGCca                                                                                                                                       >  2:299744/1‑134 (MQ=255)
ccTGAGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCca                                                                                                                                       >  2:30246/1‑134 (MQ=255)
ccTGAGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCca                                                                                                                                       >  3:186993/1‑134 (MQ=255)
ccTGAGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCca                                                                                                                                       >  5:350574/1‑134 (MQ=255)
ccTGAGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCca                                                                                                                                       >  3:252618/1‑134 (MQ=255)
ccTGAGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGAGGTCAACAGCTTGGGCca                                                                                                                                       >  1:327029/1‑134 (MQ=255)
    aGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGcc                                                                                                                                  <  3:391638/135‑1 (MQ=255)
    aGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGcc                                                                                                                                  <  1:328079/135‑1 (MQ=255)
        ccTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGc                                                                                                                             <  5:10785/136‑1 (MQ=255)
            aTGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGc                                                                                                                         >  8:173411/1‑136 (MQ=255)
              gCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGCCAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTg                                                                                                                       >  6:24906/1‑136 (MQ=255)
                      ccGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAACGc                                                                                                               >  5:171527/1‑136 (MQ=255)
                         aaCGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGtt                                                                                                            <  4:110354/136‑1 (MQ=255)
                          aCGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTg                                                                                                           >  4:39540/1‑136 (MQ=255)
                            gTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTggg                                                                                                         <  3:39540/136‑1 (MQ=255)
                            gTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTggg                                                                                                         >  1:190048/1‑136 (MQ=255)
                             tGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGGGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGc                                                                                                        <  1:81933/136‑1 (MQ=255)
                                 gcgcAGGTATTCGTGCGTGGCTAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAAcc                                                                                                    <  8:264870/136‑1 (MQ=255)
                                 gcgcAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGATGGTCAAAGCGTTGGGCAAc                                                                                                     >  1:252675/1‑135 (MQ=255)
                                   gcAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAg                                                                                                  <  5:163496/136‑1 (MQ=255)
                                           tCGTGCGTGGCCATGGTTCGTGGGTGTGGGTCAACGTAGGTCAGGCCTACCTGGTCTTCACTCAGGGCGGTGCTGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAgg                                                                                           <  5:294537/135‑1 (MQ=255)
                                               gcgtgGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGtt                                                                                       >  2:90436/1‑135 (MQ=255)
                                               gcgtgGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGtt                                                                                       >  1:10671/1‑135 (MQ=255)
                                               ccgtgGCAAGGCATCTTGTTTGTGGAACAACGAAGGCCATGCAAACATGTACTTCACACAGGCCGGTGCGGTCAACAGCTTGGACCGCAGACCCAGCGTCCGTGTCAAACCGTTGGGCAACCAGGCTAAgccttt                                                                                       >  8:344777/2‑130 (MQ=255)
                                                cgtgGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTg                                                                                      <  5:397543/135‑1 (MQ=255)
                                                   ggCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATc                                                                                   >  5:305328/1‑135 (MQ=255)
                                                     ccAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAAc                                                                                >  2:342969/1‑136 (MQ=255)
                                                     ccAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAAc                                                                                >  5:164373/1‑136 (MQ=255)
                                                     ccAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAAc                                                                                >  7:386122/1‑136 (MQ=255)
                                                         ggCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCcgg                                                                            <  5:127610/136‑1 (MQ=255)
                                                            tCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCcgggct                                                                         >  8:207591/1‑136 (MQ=255)
                                                              gTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTTGTCAACCAGTCTCAGGCGTTGATCAACCcgggctc                                                                        <  8:73584/135‑1 (MQ=255)
                                                              gTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCcgggctcg                                                                       <  3:118634/136‑1 (MQ=255)
                                                                ggTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCcgggctcggg                                                                     >  6:40575/1‑136 (MQ=255)
                                                                 gTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCcgggctcgggc                                                                    <  5:206468/136‑1 (MQ=255)
                                                                    gtgGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCtt                                                                  <  8:6544/135‑1 (MQ=255)
                                                                    gtgGGACAACGAAGGGCATGCCTACCTGGACTTCACGCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCtt                                                                  <  2:352335/135‑1 (MQ=255)
                                                                        gACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCcaca                                                             <  8:256922/136‑1 (MQ=255)
                                                                        gACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCcaca                                                             <  2:209040/136‑1 (MQ=255)
                                                                             cGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGt                                                        <  4:243409/136‑1 (MQ=255)
                                                                               aaGGGCATGCCTACCTGGACTTCACTCAGGGAGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCCTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACCACCGtcg                                                      >  2:145284/1‑134 (MQ=255)
                                                                                aGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGc                                                     >  1:105069/1‑136 (MQ=255)
                                                                                 gggCCTGCCTACCTGGACGTCACTCAGGGCGGTGAGGTCAACATCGTGGGCCACAGCCCCAGCGTGCGGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGGTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCt                                                    <  1:164602/136‑1 (MQ=255)
                                                                                 gggCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCt                                                    <  7:293883/136‑1 (MQ=255)
                                                                                     aTGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCt                                                >  7:121245/1‑136 (MQ=255)
                                                                                     aTGCCTACATGGACTTCACTCAGGGCGGTGCGGTCAAAAGCTTGGGCCGCAGCCCCAGCGTTCTGGTCAAAGCTTTGGGCAACCAGGCTCAGGAGTTGATCAACCCGCGCTAGGGCTTCCAAAACCTTgaattg                                                  >  6:99748/1‑129 (MQ=255)
                                                                                      tGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTg                                               <  1:19746/136‑1 (MQ=255)
                                                                                        ccTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTggg                                             >  6:99971/1‑136 (MQ=255)
                                                                                          tACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACACCCGTGGCTTGCTGGGGc                                           >  6:151500/1‑136 (MQ=255)
                                                                                          tACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGc                                           >  7:250744/1‑136 (MQ=255)
                                                                                            ccTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCt                                          <  1:348920/135‑1 (MQ=255)
                                                                                               ggACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGt                                       >  1:177619/1‑135 (MQ=255)
                                                                                               ggACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGt                                       >  5:311831/1‑135 (MQ=255)
                                                                                               ggACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGt                                       >  7:249163/1‑135 (MQ=255)
                                                                                                  cTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAAc                                   <  1:30246/136‑1 (MQ=255)
                                                                                                    tCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCg                                 >  5:284944/1‑136 (MQ=255)
                                                                                                    tCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCg                                 >  6:252082/1‑136 (MQ=255)
                                                                                                      aCTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCgg                                <  2:18452/135‑1 (MQ=255)
                                                                                                      aCTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGc                               >  6:59281/1‑136 (MQ=255)
                                                                                                        tCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCACCCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCtg                             >  4:58177/1‑136 (MQ=255)
                                                                                                        tCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCtg                             >  3:221301/1‑136 (MQ=255)
                                                                                                        tCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCCTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCGACAACCGTGGCTTGCTGTGGCTGGTAAACCGGCtg                             >  7:300049/1‑136 (MQ=255)
                                                                                                        tCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCCACCGGCtg                             >  2:290831/1‑136 (MQ=255)
                                                                                                          agtgcggtgctgtCAACATCTTGGGCCGCATCTACAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGTCTTGCTGGGGCTGGTCAACCGGCtgt                            <  3:238929/132‑1 (MQ=255)
                                                                                                           gggCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTgtgt                          <  2:77744/136‑1 (MQ=255)
                                                                                                            ggCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTgtgt                          >  7:252814/1‑135 (MQ=255)
                                                                                                             gcggtgcggtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTgtgt                          >  6:95643/1‑134 (MQ=255)
                                                                                                               ggtgcggtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCa                        >  5:305113/1‑134 (MQ=255)
                                                                                                                gtgcggtCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCagag                     <  7:766/136‑1 (MQ=255)
                                                                                                                   cggtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTAc                  >  1:231299/1‑136 (MQ=255)
                                                                                                                   cggtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTAc                  >  1:322417/1‑136 (MQ=255)
                                                                                                                    ggtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTAcc                 >  8:248423/1‑136 (MQ=255)
                                                                                                                    ggtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTAcc                 <  3:359948/136‑1 (MQ=255)
                                                                                                                    ggtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTAcc                 >  8:154332/1‑136 (MQ=255)
                                                                                                                     gtCTACAGCTTGGGCCTCAGGCCGAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAAGCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCTACCGGCTGTGTCAGAGTACCg                <  4:406459/136‑1 (MQ=255)
                                                                                                                     gtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCg                <  6:305328/136‑1 (MQ=255)
                                                                                                                     gtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCg                <  8:262704/136‑1 (MQ=255)
                                                                                                                     gtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCg                <  4:25198/136‑1 (MQ=255)
                                                                                                                         aCAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCACCCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAg            >  7:163804/1‑136 (MQ=255)
                                                                                                                         aCAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAg            <  6:91605/136‑1 (MQ=255)
                                                                                                                         aCAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAg            <  8:45099/136‑1 (MQ=255)
                                                                                                                          cAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGt           <  5:131596/136‑1 (MQ=255)
                                                                                                                          cAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGt           <  1:244868/136‑1 (MQ=255)
                                                                                                                           aGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGTg          <  4:141170/136‑1 (MQ=255)
                                                                                                                                 ggCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGTGACCAgg    <  8:356030/136‑1 (MQ=255)
                                                                                                                                   ccACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGTGACCAGGcc  >  7:367768/1‑136 (MQ=255)
                                                                                                                                   ccACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGTGACCAGGcc  >  3:272021/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                                     
CCTGAGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGTGACCAGGCC  >  ACB143/445226‑445492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: