Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,386,510 2 bp→TA 100% intergenic (‑519/+1131) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,386,5100AT91.3% 59.2 / ‑2.6 22intergenic (‑519/+1132)CJ019_05070/nptIIhypothetical protein/neomycin phosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (11/9);  total (12/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*ACB1435,386,5110TA95.5% 66.4 / ‑3.1 22intergenic (‑520/+1131)CJ019_05070/nptIIhypothetical protein/neomycin phosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (11/10);  minor base C (1/0);  total (12/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑C‑A‑GGATC‑‑ATCC‑GGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCT  >  ACB143/5386410‑5386607
                                                                                                           ||                                                                                                   
ccTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTGGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGc                                                                    <  5:121379/136‑1 (MQ=255)
         ttGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGaa                                                            <  4:33657/135‑1 (MQ=255)
                   aaaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGGGT‑‑‑A‑C‑GGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc                                                 <  1:143191/136‑1 (MQ=255)
                   aaaTTGATCGAATTCTTTCATTTAACACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGT‑‑‑C‑C‑GGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc                                                 <  7:362509/136‑1 (MQ=255)
                         aTCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                            <  7:212525/135‑1 (MQ=255)
                                      aTTAAAGCCCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGT‑‑‑CTACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                              <  3:286513/136‑1 (MQ=255)
                                             aCCCTAATATGACATCTCGGCCGGCTTCGCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGAACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt                       <  4:288234/136‑1 (MQ=255)
                                               ccTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTAC‑G‑GTACA‑‑AACCACGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTg                      >  8:31322/1‑135 (MQ=255)
                                                   atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg                 <  4:17800/136‑1 (MQ=255)
                                                   atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg                 <  5:299482/136‑1 (MQ=255)
                                                    tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAggg                >  7:249686/1‑136 (MQ=255)
                                                    tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAggg                >  6:90161/1‑136 (MQ=255)
                                                     atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATAACCTTACGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc               >  3:210869/1‑136 (MQ=255)
                                                            ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTTCGCTGCGGGTGCCAGGGCGTGCCCt        >  6:82164/1‑136 (MQ=255)
                                                            ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt        >  1:75963/1‑136 (MQ=255)
                                                            ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt        >  7:171506/1‑136 (MQ=255)
                                                            ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt        >  4:124443/1‑136 (MQ=255)
                                                            ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt        >  3:266685/1‑136 (MQ=255)
                                                              cggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTg      >  7:363491/1‑136 (MQ=255)
                                                               ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGc                                                                    >  4:354843/1‑73 (MQ=255)
                                                               ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGc                                                                    <  3:354843/73‑1 (MQ=255)
                                                                  cggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCATTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCt  >  2:145798/1‑136 (MQ=255)
                                                                                                           ||                                                                                                   
CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑C‑A‑GGATC‑‑ATCC‑GGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCT  >  ACB143/5386410‑5386607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑