Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 5,009,222 (A)11→10 intergenic (‑166/+68) CJ019_04705 ← / ← spuC_5 hypothetical protein/Putrescine‑‑pyruvate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,009,2120A.90.6% 200.1 / 20.1 53intergenic (‑156/+78)CJ019_04705/spuC_5hypothetical protein/Putrescine‑‑pyruvate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base . (24/24);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 3.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

ATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCT  >  ACB143/5009082‑5009340
                                                                                                                                  |                                                                                                                                
atgagatgaATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTACGTGTGTACTCATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACaaaaa                                                                                                                              >  6:214142/1‑135 (MQ=255)
   agatgaATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACaaaaaaaaa                                                                                                                          <  2:1659/136‑1 (MQ=255)
   agatgaATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACaaaaaaaa                                                                                                                           <  5:97980/135‑1 (MQ=255)
    gatgaATAATCCCGCACCGGTCTCCGGCGCGGTCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTTATTGTGATAGCACTACaaaaaaaaa                                                                                                                          <  2:1660/135‑1 (MQ=255)
    gatgaATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACaaaaaaaaa                                                                                                                          <  5:34093/135‑1 (MQ=255)
      tgaATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACaaaaaaaaaagc                                                                                                                       >  8:338401/1‑134 (MQ=255)
        aaTAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACaaaaaaaaaagccc                                                                                                                     >  8:322703/1‑132 (MQ=255)
               ccGCCCCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCGTGGACATTATCTGACCCGAAAGAACGTCCTTGTGAACTTATGACAGCGTCTTGGCTTTGATTGTGATAGCACAAAAAAAAAAAAAAGCCCGCCTaa                                                                                                              <  4:208024/136‑1 (MQ=255)
                cGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGGCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAg                                                                                                            >  1:224652/1‑136 (MQ=255)
                     cGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCggg                                                                                                       >  5:310649/1‑136 (MQ=255)
                     cGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCGAAAGGCggg                                                                                                       >  4:48633/1‑136 (MQ=255)
                             ggCGCGGGGTTTTACTTTCCGGGCCTTGGACAGAATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAAc                                                                                               <  6:6235/136‑1 (MQ=255)
                             ggCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACCGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAAc                                                                                               <  4:133931/136‑1 (MQ=255)
                             ggCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAAc                                                                                               <  1:319910/136‑1 (MQ=255)
                             ggCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAAc                                                                                               <  3:325161/136‑1 (MQ=255)
                                  gggCTTTTACTTTCCGGGCCTTGGACAGTATCTGCCCCGAAAGTACGTCCTTGTGAACTGATGCCAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGaa                                                                                            >  6:245117/1‑134 (MQ=255)
                                    gCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAagag                                                                                         >  3:105804/1‑136 (MQ=255)
                                    gCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAgg                                                                                        >  5:120146/1‑136 (MQ=255)
                                    gCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAgg                                                                                        >  5:317416/1‑136 (MQ=255)
                                                 gggCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTgg                                                                           >  3:259756/1‑136 (MQ=255)
                                                 gggCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑‑‑AAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGca                                                                         >  1:305319/1‑136 (MQ=255)
                                                  ggCCTTGGACAGTTTCTGACCCGAAAGTACGTCCTTGTGAACTGATCACCGCGTCTTGGCTTTGATTGTCATACCACTAC‑AAAAAAAAACCCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGc                                                                          <  2:183152/136‑1 (MQ=255)
                                                  ggCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGc                                                                          <  4:323691/136‑1 (MQ=255)
                                                   gCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGca                                                                         >  5:171438/1‑136 (MQ=255)
                                                             ttATCTGACCCGAAAGTACGTCCTTGTGAACTGATGAAAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCaa                                                               <  8:230045/135‑1 (MQ=255)
                                                                tCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACg                                                             <  8:236929/135‑1 (MQ=255)
                                                                    aCCCGAAAGTACGTCCTTTGGAACTGATGACCGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGt                                                            <  6:292992/133‑1 (MQ=255)
                                                                    aCCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGt                                                            <  7:47789/132‑1 (MQ=255)
                                                                     cccGAAAGTACGTCCTTGTGAACTGATTACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGACGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATg                                                       <  7:347596/136‑1 (MQ=255)
                                                                     cccGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAACAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATg                                                       <  7:256261/136‑1 (MQ=255)
                                                                      ccGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCGAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCAGCATTGCCAACGTTGATgg                                                      >  1:185840/1‑136 (MQ=255)
                                                                           aGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAAc                                                                                               >  1:67998/1‑90 (MQ=255)
                                                                           aGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAAc                                                                                               <  2:67998/90‑1 (MQ=255)
                                                                           aGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTg                                                                   >  6:367450/1‑118 (MQ=255)
                                                                           aGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTg                                                                   <  5:367450/118‑1 (MQ=255)
                                                                           aGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCt                                                    >  7:349952/1‑133 (MQ=255)
                                                                           aGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCt                                                    >  8:262469/1‑133 (MQ=255)
                                                                           aGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑‑AAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGa                                                >  8:231277/1‑136 (MQ=255)
                                                                               cGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑‑AAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCtt                                            <  8:63750/136‑1 (MQ=255)
                                                                                gTCCTTGTGAACTGATGACAGCGTGTTGGCTTTGATTGTTATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCtt                                            <  8:327432/136‑1 (MQ=255)
                                                                                gTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCtt                                            <  3:172045/136‑1 (MQ=255)
                                                                                         aaCTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCtcat                                    <  4:320373/135‑1 (MQ=255)
                                                                                          aCTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCtcatca                                  <  3:117113/136‑1 (MQ=255)
                                                                                              atgaCAGCTTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAgg                               <  6:206868/135‑1 (MQ=255)
                                                                                              atgaCAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCAGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACATTCAGCTCATCAAGGa                              >  4:68675/1‑136 (MQ=255)
                                                                                               tgaCAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAATACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGAc                             <  5:81439/136‑1 (MQ=255)
                                                                                                gaCCGCGTCTTGGCTTTGATTGTTATCGCACT‑CAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGAc                             <  3:19199/135‑1 (MQ=255)
                                                                                                 aCAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGActg                           >  3:195124/1‑134 (MQ=255)
                                                                                                    gCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTc                         <  6:105189/136‑1 (MQ=255)
                                                                                                        cTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGca                     >  8:237151/1‑135 (MQ=255)
                                                                                                              tttGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTAAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTc              >  1:341049/1‑136 (MQ=255)
                                                                                                                tGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTc              <  5:245117/134‑1 (MQ=255)
                                                                                                                 gATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCgt            <  8:349952/135‑1 (MQ=255)
                                                                                                                   ttGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGtgtg         <  3:56260/136‑1 (MQ=255)
                                                                                                                   ttGTGATAGCACTAC‑AAAAAAAAAACCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGATCAGACCTTCAGCTCATCAAGGACGCTCACCACAGGTACGtgtg         >  2:106181/1‑136 (MQ=255)
                                                                                                                    tgttttAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGtgtg         <  5:24065/130‑1 (MQ=255)
                                                                                                                     gtgATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGGGCg       >  1:227727/1‑136 (MQ=255)
                                                                                                                         tAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGc   >  1:341667/1‑136 (MQ=255)
                                                                                                                          aGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGctgtcga  >  7:124172/1‑130 (MQ=255)
                                                                                                                           gCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCt  >  6:84231/1‑135 (MQ=255)
                                                                                                                                  |                                                                                                                                
ATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCT  >  ACB143/5009082‑5009340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑