Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	587,254	T→C	intergenic (‑65/+45)	ompW ← / ← CJ019_00551	Outer membrane protein W/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	587,254	0	T	C	100.0%	195.6 / NA	50	intergenic (‑65/+45)	ompW/CJ019_00551	Outer membrane protein W/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/26);  total (24/26)

ATAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTGGAAGCTTTGACC  >  ACB143/587121‑587380                                                                                                                                      |                                                                                                                               aTAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGctt                                                                                                                              <  5:72951/136‑1 (MQ=255)  tAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTg                                                                                                                             <  4:164701/136‑1 (MQ=255)  tAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTg                                                                                                                             <  4:122809/136‑1 (MQ=255)  tAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCAACCAGCAAGGATTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAACCGGAAAGCTTg                                                                                                                             <  5:251410/136‑1 (MQ=255)           gcggcAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCt                                                                                                                     >  4:352299/1‑135 (MQ=255)                  gggCTGCGAGCGCAAGGGCCACAAGCGAGGCACCAATCAAGGACTAGTTCATGGAAGCTCCCAAAGGACGTTATTAATAGAGTAGTCCAATGGTACGGAGCTTGTTAAACGGAAAGCTTGACCCAGCTCAACGAAg                                                                                                             <  2:314767/136‑1 (MQ=255)                        cgAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCtt                                                                                                       <  8:338157/136‑1 (MQ=255)                        cgAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCtt                                                                                                       >  5:150575/1‑136 (MQ=255)                        cgAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCtt                                                                                                       <  3:327744/136‑1 (MQ=255)                        cgAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCtt                                                                                                       <  5:298473/136‑1 (MQ=255)                        cgAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCtt                                                                                                       <  8:223400/136‑1 (MQ=255)                                  ggCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCg                                                                                             <  8:188257/136‑1 (MQ=255)                                  ggCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCg                                                                                             <  7:240376/136‑1 (MQ=255)                                         aGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTCGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGt                                                                                      <  5:136181/136‑1 (MQ=255)                                              ggCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTtctca                                                                                 <  3:122154/136‑1 (MQ=255)                                              ggCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTtctca                                                                                 <  7:348331/136‑1 (MQ=255)                                              ggCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTtctca                                                                                 <  4:62312/136‑1 (MQ=255)                                              ggCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTtctca                                                                                 <  4:137203/136‑1 (MQ=255)                                              ggCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTtctca                                                                                 <  4:123198/136‑1 (MQ=255)                                              ggCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTtctca                                                                                 <  6:196795/136‑1 (MQ=255)                                              ggCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTtctca                                                                                 <  7:231390/136‑1 (MQ=255)                                                cACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTtctcatc                                                                               >  8:292271/1‑136 (MQ=255)                                                 aaccAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTtctcatct                                                                              <  4:93787/134‑1 (MQ=255)                                                        aaGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCa                                                                       >  7:341078/1‑136 (MQ=255)                                                        aaGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTCGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCa                                                                       >  2:251260/1‑136 (MQ=255)                                                          ggACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCAt                                                                      >  6:3324/1‑135 (MQ=255)                                                           gACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATaa                                                                    <  4:18950/136‑1 (MQ=255)                                                                gTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTa                                                               >  4:204294/1‑136 (MQ=255)                                                                  tCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCg                                                                 >  7:85415/1‑132 (MQ=255)                                                                  tCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCg                                                                 >  6:168995/1‑132 (MQ=255)                                                                   cATGGAAGCTCCCAAAGGACGTTAATAATCGAGTAGTCCAATGCTACGGAGCTCGATAAACGCAAAGCTAGACCCAGCTCAACCAAGCGTCTTCAAGAATTAGAAACAGATCTCATCTCAGCACATAAGCGAAGaa                                                            >  7:55331/1‑135 (MQ=255)                                                                     tGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGAttt                                                          <  2:53754/136‑1 (MQ=255)                                                                              ccAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCtt                                                 >  4:162197/1‑136 (MQ=255)                                                                              ccAAAGGACGTTATTAATCGAGTAGACCAATGGTACGGAGATGGTTAAACGGAAAGCATGACCCAGCTCAACCAAGCGTCTACACGAATACGAAACACTTCTCATCTCAGCACAAAAGCGTAGATTTTCTCGGCtt                                                 >  3:88786/1‑136 (MQ=255)                                                                                           ttaATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGcc                                    >  3:118282/1‑136 (MQ=255)                                                                                             aaTCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCg                                   <  3:352299/135‑1 (MQ=255)                                                                                                 gAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTAGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCTGCTTCCATCTGGTAGCCGGCGTc                              >  1:220633/1‑136 (MQ=255)                                                                                                      gtCCAATGGTACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCATCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGc                           >  2:292144/1‑134 (MQ=255)                                                                                                               tACGGAGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCgctgc                <  1:144620/136‑1 (MQ=255)                                                                                                                    aGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTtct                                                                                   >  4:243169/1‑64 (MQ=255)                                                                                                                    aGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTtct                                                                                   <  3:243169/64‑1 (MQ=255)                                                                                                                    aGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTgg             >  6:34775/1‑134 (MQ=255)                                                                                                                    aGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTgg             >  5:54100/1‑134 (MQ=255)                                                                                                                    aGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTgg             >  4:299307/1‑134 (MQ=255)                                                                                                                    aGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTgg             >  4:201889/1‑134 (MQ=255)                                                                                                                    aGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTgg             >  3:68372/1‑134 (MQ=255)                                                                                                                    aGCTGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTgg             >  1:210529/1‑134 (MQ=255)                                                                                                                       tGGTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTGGAAGCt        >  1:105626/1‑136 (MQ=255)                                                                                                                         gTTAAACGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTGGAAGCttt      <  1:153753/136‑1 (MQ=255)                                                                                                                            aaaCGGAAATCTTGACCCAGCTCAACGAAGCGTCTTAACCAATTACAAAACTTTATCATCTCAGCTCATAAGCGTAGATTTTCTCGGCATCCATAAGGTAGCCGGCGTCGGCCAGCACGCTGCTGGAAGCATTGAc   >  6:313650/1‑136 (MQ=255)                                                                                                                             aaCGGAAAGCTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTGGAAGCTTTGAcc  <  4:75951/136‑1 (MQ=255)                                                                                                                                      |                                                                                                                               ATAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTGGAAGCTTTGACC  >  ACB143/587121‑587380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑