| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 434,376 | 0 | G | . | 29.4% | 37.9 / 14.4 | 18 | coding (207/1173 nt) | bioF | 8‑amino‑7‑oxononanoate synthase |
| Reads supporting (aligned to +/- strand): ref base G (9/3); new base . (4/1); total (13/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCCGGAAGTGGTGGTCGAC‑GGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGG‑CCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCA > ACB143/434255‑434512 | ggCCGGAAGTGGTGGTCGACGGGCCCCCGGTTG‑GGGCGGTGTGGAGGAATGACTACCTGGGGCGGGCCCAACCA‑CCCGGGGGGATCGCCGCCAGGCAGGCCCGGGGCGCGGGGCGGGGGGGGGGGGGGGGGGcggcg > 8:30149/1‑133 (MQ=255) gtggcc‑GGCCCGCGGTTTCTGGCCTTCTGCCCCAATTCCTACCTGGGCCCGG‑CCAACCCCCCCGTGGGGTGCGCCGCCGGGCTGGCCGGCGCCGGGGCCGGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTgg < 6:80289/132‑1 (MQ=255) gcccc‑GGCCAGCGGTGGCGGGCCTTCTGCCGCAATCACTACCTGGGCCGGG‑CCAACCCCCCCGAGGTGATCGCCGCCTGGCCGGCCGGCGCCGCGCGCGGGGGTG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGcc < 7:156656/133‑1 (MQ=255) gTTTCTTGCCTTCTGCAGCAATTACAACCAGGGCCTGG‑CCAACCACCCCGACGTGATAGCCCCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCCCCCCAGGTTGTTTGCCCCACCACCCCGcc > 8:156656/1‑136 (MQ=255) tgtcTGGCCTTCTGCAGCAATGACAACCTGGGCCTGG‑CCAACCACCCCGCGGTGATCCCGGCGTGGCAGGCCGGCGCCGCGCGCT‑GGGTGGTGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAc < 6:58724/134‑1 (MQ=255) tggtGGCCTTCTTCAACAATTACTCCCTGGGCCTGG‑CCAACCCCCCCTCGGTTATCGCCGCCGGGCAGGCGGGCGCGCGGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAc < 4:148038/134‑1 (MQ=255) ttCTGCAGCAATGACTACCTGGGCCTGG‑CCAACCACCCCGTGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGGTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGtt < 1:216464/137‑1 (MQ=255) aCTACCTGGGCCTGG‑CCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCGTCGCACCTGGTAGTTGGCCCCAGCACCCCGCCCCAGCAGGTTGACGAGGcccgg > 2:229589/1‑133 (MQ=255) aCTACCTGGGCCTGG‑CCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCCCCATCAGGTTGAAGAGGCACTgg > 2:200174/1‑137 (MQ=255) cTGG‑CCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCCCACCATCAGGTTGAAGCGGCACTGGCGGAACTCAc > 7:83640/1‑137 (MQ=255) cAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGgcc > 2:35274/1‑137 (MQ=255) aaCCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCCCCCCGCACCATCAGGTTGAAGAGGCACTGGCTGCACTCACCGgccgc > 7:152157/1‑137 (MQ=255) aGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCCCCTGGTTGTGGGCCACCGCCCCCCGCCCCATCAGGTTGAAGAGGCACTGGCTGAACTCCCCGGCCGCCCGCGCGcc > 2:140888/1‑137 (MQ=255) aGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGccc > 4:106551/1‑137 (MQ=255) gccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGATGAGGCCCTGGCTGACCTCCCCGGCCGCCCGCGCGCCCTgttgtt > 4:112840/1‑137 (MQ=255) gccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTgttgtt > 6:29300/1‑137 (MQ=255) ccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCGGGTAGTTGGCCCCAGCACCCCGCACCATCAGGTGTAAGAGGCACTGGCTGAACTCACCCGCCGCCCGCGCGCCCTgtggtta > 8:218979/1‑136 (MQ=255) gcTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCCCGCCCGGTTGTTATCCACCGGCTATATGGCCAATCTg > 1:36177/1‑137 (MQ=255) tgtgGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCCCAGCACCCCGCACCATCAGGTTGAAGCGGCCCTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAAACTGGGCGCCa > 1:28271/1‑137 (MQ=255) gggggggggggCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTATCAACCGGCTATATGGCCAATCTGGGCGCCATCa > 7:204229/1‑137 (MQ=255) | GGCCGGAAGTGGTGGTCGAC‑GGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGG‑CCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCA > ACB143/434255‑434512 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |